1989
Vascular endothelial cells enhance T cell responses by markedly augmenting IL-2 concentrations
Guinan E, Smith B, Doukas J, Miller R, Pober J. Vascular endothelial cells enhance T cell responses by markedly augmenting IL-2 concentrations. Cellular Immunology 1989, 118: 166-177. PMID: 2562928, DOI: 10.1016/0008-8749(89)90366-3.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsIL-2 concentrationsPolyclonal mitogen phytohemagglutininT cell responsesEndothelial cellsBlood monocytesUnfractionated peripheral blood mononuclear cellsCell responsesPrimary allogeneic responseSuboptimal PHA concentrationsExogenous IL-2Blood mononuclear cellsT cell populationsRecombinant IL-1Cultured human endothelial cellsDermal fibroblastsIL-2 synthesisVascular endothelial cellsQuantity of antigenHuman endothelial cellsAllogeneic responseMitogen phytohemagglutininMononuclear cellsIL-2Presence of EC
1986
FUNCTIONAL ASSESSMENT OF T CELL DEPLETION FROM BONE MARROW PRIOR TO THERAPEUTIC TRANSPLANTATION USING LIMITING DILUTION CULTURE METHODS
Rozans M, Smith B, Emerson S, Crimmins M, Laurent G, Reichert T, Burakoff S, Miller R. FUNCTIONAL ASSESSMENT OF T CELL DEPLETION FROM BONE MARROW PRIOR TO THERAPEUTIC TRANSPLANTATION USING LIMITING DILUTION CULTURE METHODS. Transplantation 1986, 42: 380-386. PMID: 3094209, DOI: 10.1097/00007890-198610000-00010.Peer-Reviewed Original ResearchConceptsT-cell depletionT cellsComplement-mediated depletionCell depletionFACS analysisIL-2Functional impairmentT-lymphocyte depletionT-cell contaminationBone marrow inoculumT cell setsComplement-mediated lysisDilution methodFlow cytometric methodLymphocyte depletionComplement treatmentMarrow inoculumCytotoxic effectorsInterleukin-2Functional assessmentT101 immunotoxinCulture methodCytometric methodCell contaminationEventual death