2022
Macrophage-Derived 25-Hydroxycholesterol Promotes Vascular Inflammation, Atherogenesis, and Lesion Remodeling
Canfrán-Duque A, Rotllan N, Zhang X, Andrés-Blasco I, Thompson B, Sun J, Price N, Fernández-Fuertes M, Fowler J, Gómez-Coronado D, Sessa W, Giannarelli C, Schneider R, Tellides G, McDonald J, Fernández-Hernando C, Suárez Y. Macrophage-Derived 25-Hydroxycholesterol Promotes Vascular Inflammation, Atherogenesis, and Lesion Remodeling. Circulation 2022, 147: 388-408. PMID: 36416142, PMCID: PMC9892282, DOI: 10.1161/circulationaha.122.059062.Peer-Reviewed Original ResearchConceptsLipid-loaded macrophagesLineage-tracing mouse modelsSREBP transcriptional activityCholesterol biosynthetic intermediatesWestern diet feedingAccessible cholesterolDifferent macrophage populationsTranscriptomic analysisKey immune regulatorsPlasma membraneAtherosclerosis progressionImmune activationTranscriptional activityGene expressionDiet feedingInflammatory responseMouse bone marrowLiver X receptorBiosynthetic intermediatesSterol metabolismApoptosis susceptibilityToll-like receptor 4Proinflammatory gene expressionHuman coronary atherosclerotic lesionsMouse atherosclerotic plaques
2006
Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase
Fernández-Hernando C, Fukata M, Bernatchez PN, Fukata Y, Lin MI, Bredt DS, Sessa WC. Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase. Journal Of Cell Biology 2006, 174: 369-377. PMID: 16864653, PMCID: PMC2064233, DOI: 10.1083/jcb.200601051.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsComplementary DNAPalmitoylation-deficient mutantHuman embryonic kidney 293 cellsEmbryonic kidney 293 cellsEndothelial nitric oxide synthaseEndothelial cellsKidney 293 cellsDHHC enzymesN-myristoylationS-palmitoylationNew GolgiSubcellular localizationCDNA clonesPlasma membraneLipid modificationCytoplasmic aspectENOS localizationGolgi apparatusRegulatory roleENOS palmitoylationPalmitoyl transferaseGolgiNitric oxide synthaseAcyl transferase