Development of a Quantitative PCR Assay for Detection of Human Insulin-Like Growth Factor Receptor and Insulin Receptor Isoforms
Flannery CA, Rowzee AM, Choe GH, Saleh FL, Radford CC, Taylor HS, Wood TL. Development of a Quantitative PCR Assay for Detection of Human Insulin-Like Growth Factor Receptor and Insulin Receptor Isoforms. Endocrinology 2016, 157: 1702-1708. PMID: 26862994, PMCID: PMC4816738, DOI: 10.1210/en.2015-1698.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA PrimersHumansProtein IsoformsReceptor, IGF Type 1Receptor, InsulinReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerConceptsSplice variantsInsulin-like growth factor (IGF) ligandsInsulin-like growth factor receptorGrowth factor ligandsSpecific molecular signaturesQuantitative PCR assaysGrowth factor receptorHigh homologyMRNA sequencesQuantitative RT-PCRIGF-1 receptorFactor ligandInsulin receptor isoformsCompetition assaysPrimer pairsRelative abundanceInsulin receptorMolecular signaturesCell typesDifferent tissuesIGF-1R mRNAFactor receptorExpression levelsGel electrophoresisReceptor isoforms