2010
Acute Down-regulation of Sodium-dependent Phosphate Transporter NPT2a Involves Predominantly the cAMP/PKA Pathway as Revealed by Signaling-selective Parathyroid Hormone Analogs
Nagai S, Okazaki M, Segawa H, Bergwitz C, Dean T, Potts JT, Mahon MJ, Gardella TJ, Jüppner H. Acute Down-regulation of Sodium-dependent Phosphate Transporter NPT2a Involves Predominantly the cAMP/PKA Pathway as Revealed by Signaling-selective Parathyroid Hormone Analogs. Journal Of Biological Chemistry 2010, 286: 1618-1626. PMID: 21047792, PMCID: PMC3020770, DOI: 10.1074/jbc.m110.198416.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleChlorocebus aethiopsCOS CellsCyclic AMPCyclic AMP-Dependent Protein KinasesDown-RegulationHumansIn Vitro TechniquesKidney Tubules, ProximalMaleMiceMice, Inbred C57BLOpossumsOsteoblastsParathyroid HormonePhosphorusPseudohypoparathyroidismRatsSignal TransductionSodiumSodium-Phosphate Cotransporter Proteins, Type IIaConceptsAcute down-regulationNpt2a expressionParathyroid hormoneRenal proximal tubule cellsParathyroid hormone (PTH)/PTH-related peptideCAMP/PKALong-acting PTH analogPTH analogsWild-type miceRenal proximal tubulesIntracellular calcium responsesParathyroid hormone analogProximal tubule cellsOpossum kidney cellsM-PTH(1Prolonged cAMP responsesParathyroid hormone analoguesCAMP/PKA signaling pathwayPTH-dependent regulationRenal brush border membraneClonal cell linesInducing IP(3Pseudohypoparathyroid patientsMembrane expressionCalcium response
2002
Cyclic-adenosine 3′,5′-monophosphate-stimulated c-fos gene transcription involves distinct calcium pathways in single β-cells
Schöfl C, Waring M, Bergwitz C, Arseniev L, von zur Mühlen A, Brabant G. Cyclic-adenosine 3′,5′-monophosphate-stimulated c-fos gene transcription involves distinct calcium pathways in single β-cells. Molecular And Cellular Endocrinology 2002, 186: 121-131. PMID: 11850128, DOI: 10.1016/s0303-7207(01)00609-8.Peer-Reviewed Original ResearchConceptsSerum response elementCa(2+)-influxC-fos gene transcriptionC-fos promoterCAMP response elementC-fosBeta-cellsModes of Ca(2+) entryGene transcriptionCa(2+) entryCa(2+C-fos mRNA expressionCa(2+) signalingForskolin-induced riseCa(2+)-free mediumForskolin-induced increaseCytosolic free calciumActivation of c-fos transcriptionCa(2+)-dependent pathwaysSingle B cellsCa(2+)-mediated mechanismsBeta-cell activityC-fos promoter activityC-fos transcriptionEffect of cAMP
1997
Residues in the Membrane-spanning and Extracellular Loop Regions of the Parathyroid Hormone (PTH)-2 Receptor Determine Signaling Selectivity for PTH and PTH-related Peptide*
Bergwitz C, Jusseaume S, Luck M, Jüppner H, Gardella T. Residues in the Membrane-spanning and Extracellular Loop Regions of the Parathyroid Hormone (PTH)-2 Receptor Determine Signaling Selectivity for PTH and PTH-related Peptide*. Journal Of Biological Chemistry 1997, 272: 28861-28868. PMID: 9360953, DOI: 10.1074/jbc.272.46.28861.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell MembraneCOS CellsCyclic AMPHistidineHumansIsoleucineMolecular Sequence DataMutagenesis, Site-DirectedParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsProteinsReceptor, Parathyroid Hormone, Type 2Receptors, Parathyroid HormoneSequence Homology, Amino AcidSignal TransductionConceptsPTH-2 receptorPTH-1 receptorParathyroid hormoneCOOH-terminal portionCOS-7 cellsCassette substitutionsPTH 1Membrane-spanningPoint mutationsTransmembrane helix 3Helix 3Divergent residuesPTHCOS-7Receptor selectivityResidues 5ReceptorsPTH-related peptideFunctional interactionsPTHrP-(1-36Receptor DetermineReceptor chimerasCAMP responseExtracellular loop 2PTH-2
1994
Rapid desensitization of parathyroid hormone dependent adenylate cyclase in perifused human osteosarcoma cells (SaOS-2)
Bergwitz C, Abou-Samra A, Hesch R, Jüppner H. Rapid desensitization of parathyroid hormone dependent adenylate cyclase in perifused human osteosarcoma cells (SaOS-2). Biochimica Et Biophysica Acta 1994, 1222: 447-456. PMID: 8038214, DOI: 10.1016/0167-4889(94)90053-1.Peer-Reviewed Original ResearchConceptsApplication of parathyroid hormoneParathyroid hormonePTH(1-34CAMP releasePerifusion systemIncrease bone mass in vivoHormone-dependent adenylate cyclaseAdenylate cyclaseSaos-2 cellsReceptor internalizationPMA pre-treatmentDose-dependent increaseCyclic AMP accumulationBone mass in vivoStimulated cAMP accumulationHuman osteosarcoma cell line Saos-2Saos-2Osteosarcoma cell line Saos-2Cells in vitroMass in vivoBone massPerifusion experimentsBPTH(3-34Human osteosarcoma cellsHomologous desensitization