2011
Phosphate Sensing
Bergwitz C, Jüppner H. Phosphate Sensing. Advances In Kidney Disease And Health 2011, 18: 132-144. PMID: 21406298, PMCID: PMC3059779, DOI: 10.1053/j.ackd.2011.01.004.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsAmino acid sequence conservationNumerous cellular functionsMulti-protein complexesDifferent signal transduction cascadesSignal transduction cascadeExpression of genesSignal transduction cascadesEukaryotic organismsMulticellular organismsMetazoan cellsMetazoan orthologsSequence conservationCellular functionsPHO pathwayTransduction cascadePlasma membraneAmbient phosphateCell metabolismExtracellular phosphateYeastGrowth of tissuesPhosphate sensorPhosphate homeostasisPhosphate uptakeCirculating phosphate levels
2002
Cyclic-adenosine 3′,5′-monophosphate-stimulated c-fos gene transcription involves distinct calcium pathways in single β-cells
Schöfl C, Waring M, Bergwitz C, Arseniev L, von zur Mühlen A, Brabant G. Cyclic-adenosine 3′,5′-monophosphate-stimulated c-fos gene transcription involves distinct calcium pathways in single β-cells. Molecular And Cellular Endocrinology 2002, 186: 121-131. PMID: 11850128, DOI: 10.1016/s0303-7207(01)00609-8.Peer-Reviewed Original ResearchConceptsSerum response elementCa(2+)-influxC-fos gene transcriptionC-fos promoterCAMP response elementC-fosBeta-cellsModes of Ca(2+) entryGene transcriptionCa(2+) entryCa(2+C-fos mRNA expressionCa(2+) signalingForskolin-induced riseCa(2+)-free mediumForskolin-induced increaseCytosolic free calciumActivation of c-fos transcriptionCa(2+)-dependent pathwaysSingle B cellsCa(2+)-mediated mechanismsBeta-cell activityC-fos promoter activityC-fos transcriptionEffect of cAMP
2000
A versatile chondrogenic rat calvaria cell line R-tTA-24 that permits tetracycline-regulated gene expression
Bergwitz C, Wendlandt T, Pötter E, Glomb I, Gras K, von zur Mühlen A, Brabant G. A versatile chondrogenic rat calvaria cell line R-tTA-24 that permits tetracycline-regulated gene expression. Histochemistry And Cell Biology 2000, 113: 145-150. PMID: 10766267, DOI: 10.1007/s004180050017.Peer-Reviewed Original ResearchConceptsTetracycline-regulated gene expressionGene expressionTetracycline-regulated gene expression systemStable transfectionChondrogenic differentiationGene expression systemGene of interestBasis of phenotypeTetracycline transactivatorGenetic manipulationHygromycin selectionExpression systemCartilage-specific proteoglycansNeomycin selectionFlexible genetic manipulationLuciferase activityType II expressionCell linesAlcian blue stainingDifferentiationCollagen type II expressionTransfectionExpressionBlue stainingGenes