Phorbol ester stimulates a protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product.
Alessandrini A, Crews CM, Erikson RL. Phorbol ester stimulates a protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 8200-8204. PMID: 1518847, PMCID: PMC49885, DOI: 10.1073/pnas.89.17.8200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCells, CulturedIn Vitro TechniquesMiceMitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMolecular Sequence DataMutagenesis, Site-DirectedOligodeoxyribonucleotidesPeptide MappingPhorbol EstersPhosphorylationPhosphothreonineProtein KinasesProtein-Tyrosine KinasesT-LymphocytesConceptsProtein kinase activityProtein kinaseGene productsKinase activityMyelin basic protein kinaseMyelin basic protein kinase activityMultiple extracellular signalsUpstream protein kinaseWild-type proteinIdentification of proteinsAmino acid residuesSame amino acid residuesERK-1 proteinDegree of phosphorylationReversible phosphorylationThreonine sitesThreonine kinaseExtracellular signalsTyrosine sitesAcid residuesKinasePhosphorylationPhorbol esterProteinThreonineErks: their fifteen minutes has arrived.
Crews CM, Alessandrini A, Erikson RL. Erks: their fifteen minutes has arrived. Molecular Cancer Research 1992, 3: 135-42. PMID: 1504018.Peer-Reviewed Original ResearchConceptsProtein kinaseCell cycleYeast cellsTyrosine kinase signalsERK protein kinasesSea star oocytesSpecific transcriptional factorsAmino acid residuesSpecific differentiation eventsG0-G1 transitionExtracellular signalsKinase signalsPhosphorylation signalsSignal transductionTranscriptional changesS6 kinaseRaf-1Differentiation eventsMitogenic signalsYeast enzymeGene productsMicrotubule reorganizationDownstream targetsTranscriptional factorsEGF receptor