2017
Single methyl groups can act as toggle switches to specify transmembrane Protein-protein interactions
He L, Steinocher H, Shelar A, Cohen EB, Heim EN, Kragelund BB, Grigoryan G, DiMaio D. Single methyl groups can act as toggle switches to specify transmembrane Protein-protein interactions. ELife 2017, 6: e27701. PMID: 28869036, PMCID: PMC5597333, DOI: 10.7554/elife.27701.Peer-Reviewed Original ResearchConceptsProtein-protein interactionsErythropoietin receptorTransmembrane proteinTransmembrane protein-protein interactionsTMD interactionsModel transmembrane proteinMouse erythropoietin receptorHuman erythropoietin receptorSingle methyl groupGrowth factor independenceSide chain methyl groupsCellular processesMouse cellsFactor independenceChain methyl groupsIntrinsic specificityToggle switchTraptamersMethyl groupProteinReceptor activitySpecific positionsReceptorsSpecificityOligomerizationTwo transmembrane dimers of the bovine papillomavirus E5 oncoprotein clamp the PDGF β receptor in an active dimeric conformation
Karabadzhak AG, Petti LM, Barrera FN, Edwards APB, Moya-Rodríguez A, Polikanov YS, Freites JA, Tobias DJ, Engelman DM, DiMaio D. Two transmembrane dimers of the bovine papillomavirus E5 oncoprotein clamp the PDGF β receptor in an active dimeric conformation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: e7262-e7271. PMID: 28808001, PMCID: PMC5584431, DOI: 10.1073/pnas.1705622114.Peer-Reviewed Original ResearchConceptsTransmembrane domainE5 proteinE5 dimerPlatelet-derived growth factor β receptorGrowth factor β receptorActive dimeric conformationPDGF β-receptorTransmembrane dimerProtein bindsMembrane environmentReceptor dimerizationDimeric conformationAtom molecular dynamics simulationsBiochemical experimentsMouse cellsMolecular mechanismsActive dimerΒ receptorBovine papillomavirusProteinSpecific interactionsMembrane modelingReceptor activationDimerizationComplexes
1992
Stable association between the bovine papillomavirus E5 transforming protein and activated platelet-derived growth factor receptor in transformed mouse cells.
Petti L, DiMaio D. Stable association between the bovine papillomavirus E5 transforming protein and activated platelet-derived growth factor receptor in transformed mouse cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 6736-6740. PMID: 1323117, PMCID: PMC49578, DOI: 10.1073/pnas.89.15.6736.Peer-Reviewed Original ResearchConceptsE5 proteinPlatelet-derived growth factorGrowth factor receptor activationPDGF receptorMouse C127 cellsBovine papillomavirus E5Platelet-derived growth factor receptorShorter proteinTransforming proteinCoimmunoprecipitation analysisGrowth factor receptorReceptor transmitsStable associationC127 cellsTumorigenic transformationMouse cellsProteinBovine papillomavirusFactor receptorDistinct mechanismsStable complexesGrowth factorReceptor activationImportant targetBeta receptors
1989
Structure, Activity, and Regulation of the Bovine Papillomavirus E5 Gene and Its Transforming Protein Product
Horwitz B, Settleman J, Prakash S, DiMaio D. Structure, Activity, and Regulation of the Bovine Papillomavirus E5 Gene and Its Transforming Protein Product. Current Topics In Microbiology And Immunology 1989, 144: 143-151. PMID: 2551579, DOI: 10.1007/978-3-642-74578-2_18.Peer-Reviewed Original ResearchConceptsE5 geneFoci formationBovine papillomavirus type 1 DNACell focus formationBPV geneCodon resultsC127 cellsMouse cellsTumorigenic transformationProtein productsK polypeptideGenesViral mutantsBiochemical analysisEfficient transformationCellsMutantsPolypeptideDNARegulationExpressionDownstreamActivityTranslationFormation
1984
High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector.
DiMaio D, Corbin V, Sibley E, Maniatis T. High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector. Molecular And Cellular Biology 1984, 4: 340-350. PMID: 6321959, PMCID: PMC368701, DOI: 10.1128/mcb.4.2.340.Peer-Reviewed Original ResearchConceptsHLA antigensBovine papillomavirus vectorCell linesFunction of HLA antigensHuman heavy chainsBovine papillomavirusHeavy chainHuman lymphoblastoid cell linesHeavy chain geneMouse cellsDNA expression vectorsLymphoblastoid cell linesTransformed cellsJY cell lineExtrachromosomal viral DNAHuman histocompatibility antigensSelection in vivoHistocompatibility antigensTransformed cell linesHLA genesChain geneCultured mouse cellsHLAHigh-level expressionAntigenHigh-Level Expression of a Cloned HLA Heavy Chain Gene Introduced into Mouse Cells on a Bovine Papillomavirus Vector
DiMaio D, Corbin V, Sibley E, Maniatis T. High-Level Expression of a Cloned HLA Heavy Chain Gene Introduced into Mouse Cells on a Bovine Papillomavirus Vector. Molecular And Cellular Biology 1984, 4: 340-350. DOI: 10.1128/mcb.4.2.340-350.1984.Peer-Reviewed Original ResearchHLA antigensBovine papillomavirus vectorCell linesFunction of HLA antigensHuman heavy chainsBovine papillomavirusHeavy chainHuman lymphoblastoid cell linesHeavy chain geneMouse cellsDNA expression vectorsLymphoblastoid cell linesTransformed cellsJY cell lineExtrachromosomal viral DNAHuman histocompatibility antigensSelection in vivoHistocompatibility antigensTransformed cell linesHLA genesChain geneCultured mouse cellsHLAHigh-level expressionAntigen
1983
Identification of two distinct regulatory regions adjacent to the human β-interferon gene
Zinn K, Dimaio D, Maniatis T. Identification of two distinct regulatory regions adjacent to the human β-interferon gene. Cell 1983, 34: 865-879. PMID: 6313211, DOI: 10.1016/0092-8674(83)90544-5.Peer-Reviewed Original ResearchConceptsIFN gene expressionBovine papilloma virusRegulatory regionsExpression of deletion mutantsGene expressionMRNA cap sitePoly(I)-poly(CCell linesStable cell linesIndependent cell linesCloned geneStructural geneDeletion mutantsLevel of expressionKinetics of inductionCap siteMouse cellsHuman beta-interferonGenesInduced expressionConstitutive levelsDeletionBeta-interferonExpressionPapilloma virus
1982
Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells.
DiMaio D, Treisman R, Maniatis T. Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 4030-4034. PMID: 6287462, PMCID: PMC346570, DOI: 10.1073/pnas.79.13.4030.Peer-Reviewed Original ResearchConceptsMammalian cellsBacterial cellsInduce focus formationHuman beta-globin gene clusterBeta-globin gene clusterBPV DNADerivative of pBR322Mouse C127 cellsTransformation of bacteriaTransformed mouse cellsHuman beta-globin geneExtrachromosomal elementsGene clusterBeta-globin geneBovine papillomavirus vectorDeletion derivativesInput DNACellular genesRecombinant plasmidC127 cellsCopy numberMouse cellsTransformed cellsPlasmidFoci formation