2001
Induced senescence in HeLa cervical carcinoma cells containing elevated telomerase activity and extended telomeres.
Goodwin E, DiMaio D. Induced senescence in HeLa cervical carcinoma cells containing elevated telomerase activity and extended telomeres. Molecular Cancer Research 2001, 12: 525-34. PMID: 11714633.Peer-Reviewed Original ResearchConceptsTumor suppressor pathwayHeLa cervical carcinoma cellsExtended telomeresReplicative senescenceHTERT geneSuppressor pathwayTelomerase activityGrowth arrestCervical carcinoma cellsRepression of telomeraseElevated telomerase activitySomatic human cellsNormal somatic human cellsShort telomeresGrowth-arrested stateSenescence-associated beta-galactosidase expressionHPV E6/E7 expressionGrowth-arrested cellsHeLa cell clonesProfound growth arrestRole of telomeraseE6/E7 genesBeta-galactosidase expressionErosion of telomeresCarcinoma cells
2000
Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways
Goodwin E, DiMaio D. Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 12513-12518. PMID: 11070078, PMCID: PMC18795, DOI: 10.1073/pnas.97.23.12513.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBovine papillomavirus 1Carrier ProteinsCattleCell Cycle ProteinsCyclin-Dependent Kinase Inhibitor p21CyclinsDNADNA-Binding ProteinsE2F Transcription FactorsFemaleGene Expression Regulation, ViralGenes, Tumor SuppressorHeLa CellsHumansNuclear ProteinsOncogene Proteins, ViralOncogenesPapillomaviridaePapillomavirus E7 ProteinsPhosphoproteinsProteinsProto-Oncogene ProteinsProto-Oncogene Proteins c-mdm2Repressor ProteinsRetinoblastoma ProteinRetinoblastoma-Binding Protein 1Retinoblastoma-Like Protein p107Retinoblastoma-Like Protein p130Signal TransductionTranscription Factor DP1Transcription FactorsTumor Suppressor Protein p53Uterine Cervical NeoplasmsViral ProteinsConceptsTumor suppressor pathwayE6/E7 repressionPosttranscriptional inductionSuppressor pathwayBovine papillomavirus E2 proteinE7 repressionCyclin-dependent kinase activityHeLa cellsE2F-regulated genesE2F-responsive genesRb tumor suppressor pathwayPapillomavirus E2 proteinCell cycle machineryE2 proteinHPV16 E6/E7 genesHeLa cervical carcinoma cellsP53-responsive genesTumor suppressor functionHPV E6Growth inhibitory signalsE6/E7 genesRapid repressionCellular DNA synthesisCycle machineryHuman papillomavirus oncogenes
1998
Transactivation-Competent Bovine Papillomavirus E2 Protein Is Specifically Required for Efficient Repression of Human Papillomavirus Oncogene Expression and for Acute Growth Inhibition of Cervical Carcinoma Cell Lines
Goodwin E, Naeger L, Breiding D, Androphy E, DiMaio D. Transactivation-Competent Bovine Papillomavirus E2 Protein Is Specifically Required for Efficient Repression of Human Papillomavirus Oncogene Expression and for Acute Growth Inhibition of Cervical Carcinoma Cell Lines. Journal Of Virology 1998, 72: 3925-3934. PMID: 9557678, PMCID: PMC109618, DOI: 10.1128/jvi.72.5.3925-3934.1998.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesBovine papillomavirus 1CattleCell DivisionCell NucleusCOS CellsDNADNA-Binding ProteinsFemaleGene Expression Regulation, ViralHeLa CellsHumansMutagenesisOncogene Proteins, ViralOncogenesPapillomaviridaeRepressor ProteinsRNA, MessengerRNA, ViralTrans-ActivatorsTranscriptional ActivationTumor Cells, CulturedUterine Cervical NeoplasmsViral ProteinsConceptsPapillomavirus E2 proteinGrowth arrestHT-3 cellsEfficient repressionTransactivation domainE2 proteinHeLa cellsG1/S-phase growth arrestE2 mutantsBovine papillomavirus type 1 E2 proteinBovine papillomavirus E2 proteinHerpes simplex virus VP16Reporter plasmidAcute growth inhibitionE2 transactivation domainGrowth inhibitionCervical carcinoma cell linesBPV1 E2 proteinCarcinoma cell linesHuman papillomavirus oncogene expressionViral DNA replicationPhase growth arrestSequence-specific transactivatorCell linesWild-type p53 gene
1994
Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes
Kegler-Ebo D, Docktor C, DiMaio D. Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes. Nucleic Acids Research 1994, 22: 1593-1599. PMID: 8202358, PMCID: PMC308034, DOI: 10.1093/nar/22.9.1593.Peer-Reviewed Original Research
1992
The central hydrophobic domain of the bovine papillomavirus E5 transforming protein can be functionally replaced by many hydrophobic amino acid sequences containing a glutamine
Kulke R, Horwitz B, Zibello T, DiMaio D. The central hydrophobic domain of the bovine papillomavirus E5 transforming protein can be functionally replaced by many hydrophobic amino acid sequences containing a glutamine. Journal Of Virology 1992, 66: 505-511. PMID: 1727496, PMCID: PMC238311, DOI: 10.1128/jvi.66.1.505-511.1992.Peer-Reviewed Original ResearchConceptsHydrophobic amino acidsAmino acidsE5 proteinRandom hydrophobic sequencesHydrophobic domainRodent fibroblast cell linesCentral hydrophobic domainHydrophobic amino acid sequenceCarboxyl-terminal amino acidsMouse C127 cellsAmino acid sequenceClasses of mutantsAbsence of glutamineBovine papillomavirus E5Mutant proteinsTransforming proteinDefective mutantsHydrophobic sequenceFibroblast cell lineProtein stabilityAcid sequenceC127 cellsHomodimer formationEfficient transformationProtein
1991
Biological properties of the deer papillomavirus E5 gene in mouse C127 cells: growth transformation, induction of DNA synthesis, and activation of the platelet-derived growth factor receptor
Kulke R, DiMaio D. Biological properties of the deer papillomavirus E5 gene in mouse C127 cells: growth transformation, induction of DNA synthesis, and activation of the platelet-derived growth factor receptor. Journal Of Virology 1991, 65: 4943-4949. PMID: 1651413, PMCID: PMC248956, DOI: 10.1128/jvi.65.9.4943-4949.1991.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBlotting, NorthernCell Transformation, ViralCloning, MolecularDeerDNAGene ExpressionGenes, ViralIn Vitro TechniquesMiceMolecular Sequence DataOncogene Proteins, ViralPapillomaviridaePlatelet-Derived Growth FactorReceptors, Cell SurfaceReceptors, Platelet-Derived Growth FactorRNA, ViralViral Structural ProteinsConceptsMouse C127 cellsE5 proteinC127 cellsE5 genePlatelet-derived growth factor beta receptorPDGF receptorBovine papillomavirus type 1 E5 proteinConstitutive tyrosine phosphorylationDNA synthesisGrowth factor beta receptorBovine papillomavirus type 1Platelet-derived growth factor receptorTransformation of fibroblastsPapillomavirus type 1Sequence similarityGrowth factor receptorTyrosine phosphorylationBiological activityShort regionFoci formationProteinFactor receptorReceptor formsB chainGrowth transformation
1988
A model system for the rescue of cDNA encoding transacting transcription activator by contingent replication assay.
Vasavada H, Ganguly S, Settleman J, Dimaio D, Weissman S. A model system for the rescue of cDNA encoding transacting transcription activator by contingent replication assay. Indian Journal Of Biochemistry And Biophysics 1988, 25: 488-94. PMID: 2855627.Peer-Reviewed Original Research
1983
Identification of two distinct regulatory regions adjacent to the human β-interferon gene
Zinn K, Dimaio D, Maniatis T. Identification of two distinct regulatory regions adjacent to the human β-interferon gene. Cell 1983, 34: 865-879. PMID: 6313211, DOI: 10.1016/0092-8674(83)90544-5.Peer-Reviewed Original ResearchConceptsIFN gene expressionBovine papilloma virusRegulatory regionsExpression of deletion mutantsGene expressionMRNA cap sitePoly(I)-poly(CCell linesStable cell linesIndependent cell linesCloned geneStructural geneDeletion mutantsLevel of expressionKinetics of inductionCap siteMouse cellsHuman beta-interferonGenesInduced expressionConstitutive levelsDeletionBeta-interferonExpressionPapilloma virus
1981
Directed Mutagenesis
Shortle D, DiMaio D, Nathans D. Directed Mutagenesis. Annual Review Of Genetics 1981, 15: 265-294. PMID: 6279018, DOI: 10.1146/annurev.ge.15.120181.001405.Peer-Reviewed Original Research