Featured Publications
Mechanism and Regulation of Centriole and Cilium Biogenesis
Breslow DK, Holland AJ. Mechanism and Regulation of Centriole and Cilium Biogenesis. Annual Review Of Biochemistry 2019, 88: 1-34. PMID: 30601682, PMCID: PMC6588485, DOI: 10.1146/annurev-biochem-013118-111153.ChaptersConceptsInterphase microtubule cytoskeletonMicrotubule-based organellesBiogenesis of centriolesMost animal cellsCore of centrosomesFormation of ciliaNine-fold symmetryCilia biologyCilia biogenesisCellular signalingMicrotubule cytoskeletonAnimal cellsMitotic spindleBasal bodiesHuman diseasesCentriolesBiogenesisRegulatory controlCentral roleCiliaExciting avenuesCentrosomesCytoskeletonOrganellesSignalingA CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies
Breslow DK, Hoogendoorn S, Kopp AR, Morgens DW, Vu BK, Kennedy MC, Han K, Li A, Hess GT, Bassik MC, Chen JK, Nachury MV. A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies. Nature Genetics 2018, 50: 460-471. PMID: 29459677, PMCID: PMC5862771, DOI: 10.1038/s41588-018-0054-7.Peer-Reviewed Original ResearchConceptsFunctional genomic screensGenome-wide CRISPRCiliary functionHedgehog-responsive cellsCiliary signalingΕ-tubulinProtein complexesGenomic screenEmbryonic developmentGene disruptionPrimary ciliaΔ-tubulinNovel componentCiliopathiesCRISPRCiliary structureUnbiased toolHedgehogUnifying causeScreenGenesSignalingCiliaSystematic analysisPathwayAn in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier
Breslow DK, Koslover EF, Seydel F, Spakowitz AJ, Nachury MV. An in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier. Journal Of Cell Biology 2013, 203: 129-147. PMID: 24100294, PMCID: PMC3798247, DOI: 10.1083/jcb.201212024.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsCell LineCell MembraneCell Membrane PermeabilityCiliaDiffusionMiceMicroscopy, FluorescenceMicroscopy, VideoModels, BiologicalMolecular WeightNuclear PoreProtein TransportProteinsRecombinant Fusion ProteinsReproducibility of ResultsTime FactorsTime-Lapse ImagingTransfectionConceptsNuclear pore complexCiliary diffusion barrierPore complexActin cytoskeletonMembrane proteinsActive transportPrimary ciliaPlasma membraneCiliary membraneSpecific proteinsLarge proteinsMechanistic basisPermeabilized cellsProteinCiliaAxon initial segmentMembraneCellsCytoskeletonInitial segmentEntryTransportAssaysVivoComplexes
2015
Chapter 11 Analysis of soluble protein entry into primary cilia using semipermeabilized cells
Breslow DK, Nachury MV. Chapter 11 Analysis of soluble protein entry into primary cilia using semipermeabilized cells. Methods In Cell Biology 2015, 127: 203-221. PMID: 25837393, PMCID: PMC4797650, DOI: 10.1016/bs.mcb.2014.12.006.BooksConceptsSemipermeabilized cellsProtein entriesPrimary ciliaCiliary diffusion barrierNuclear pore complexPrimary cilia functionPore complexMammalian cellsSignal transductionSpecialized compartmentsCilia functionPlasma membraneCiliary membraneIntact cellsExperimental perturbationsCell surfaceProtein exchangeCiliaVitro systemAxon initial segmentMechanistic analysisChapter 11 AnalysisUnique resourceCapture assayCells
2014
The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3
Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP, Nachury MV. The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3. Developmental Cell 2014, 31: 265-278. PMID: 25443296, PMCID: PMC4255629, DOI: 10.1016/j.devcel.2014.09.004.Peer-Reviewed Original ResearchConceptsCargo entryBardet-Biedl syndrome proteinsIntraflagellar transport (IFT) machineryBiochemical reconstitution assaysNucleotide-free formCiliary exitTransport machinerySyndrome proteinGTP loadingUnbiased proteomicsCoat assemblyReconstitution assaysBBSomeARL6IFT27CiliaBBS3AssemblyProteomicsMachineryProteinCargoSortingExitActivation
2013
Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors
Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ, Nachury MV. Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors. ELife 2013, 2: e00654. PMID: 23930224, PMCID: PMC3736543, DOI: 10.7554/elife.00654.Peer-Reviewed Original ResearchConceptsCiliary membrane proteinsIntraflagellar transportIFT trainsMembrane proteinsMembrane protein diffusionSingle-molecule imagingSomatostatin receptor 3Active transportPrimary ciliaCiliary membraneMolecule imagingProtein diffusionDynamic organizationDirectional movementReceptor 3ProteinCiliaPossible roleStatistical subtractionSingle moleculesMajor roleSmoothenedSMOSSTR3Diffusive behavior
2011
A Novel Protein LZTFL1 Regulates Ciliary Trafficking of the BBSome and Smoothened
Seo S, Zhang Q, Bugge K, Breslow DK, Searby CC, Nachury MV, Sheffield VC. A Novel Protein LZTFL1 Regulates Ciliary Trafficking of the BBSome and Smoothened. PLOS Genetics 2011, 7: e1002358. PMID: 22072986, PMCID: PMC3207910, DOI: 10.1371/journal.pgen.1002358.Peer-Reviewed Original ResearchConceptsCiliary traffickingBBS proteinsBardet-Biedl syndrome proteinsLeucine zipper transcriptionHedgehog signal transducerG protein-coupled receptorsProtein-coupled receptorsCiliary entryBBSome subunitsProtein traffickingSyndrome proteinProtein complexesCellular processesNovel proteinPrimary ciliaHedgehog signalingSignal transducerBBSomeImportant regulatorTraffickingLZTFL1Ciliary functionProteinBBS3CiliaPrimary Cilia: How to Keep the Riff-Raff in the Plasma Membrane
Breslow DK, Nachury MV. Primary Cilia: How to Keep the Riff-Raff in the Plasma Membrane. Current Biology 2011, 21: r434-r436. PMID: 21640903, DOI: 10.1016/j.cub.2011.04.039.Commentaries, Editorials and Letters