2009
Structural basis of competition between PINCH1 and PINCH2 for binding to the ankyrin repeat domain of integrin-linked kinase
Chiswell BP, Stiegler AL, Razinia Z, Nalibotski E, Boggon TJ, Calderwood DA. Structural basis of competition between PINCH1 and PINCH2 for binding to the ankyrin repeat domain of integrin-linked kinase. Journal Of Structural Biology 2009, 170: 157-163. PMID: 19963065, PMCID: PMC2841223, DOI: 10.1016/j.jsb.2009.12.002.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAmino Acid SequenceAnkyrin RepeatBinding, CompetitiveCrystallizationDNA-Binding ProteinsGene Expression RegulationLIM Domain ProteinsMembrane ProteinsModels, MolecularMolecular Sequence DataMutagenesisProtein BindingProtein Serine-Threonine KinasesSignal TransductionConceptsIntegrin-linked kinaseAnkyrin repeat domainLIM1 domainIPP complexIsoform-specific functionsIntegrin adhesion receptorsDifferent cellular responsesPINCH2Repeat domainPINCH1Point mutagenesisStructural basisAdhesion receptorsCellular responsesAlters localizationDifferential regulationSame binding siteDirect competitionBinding sitesKinaseDomainAnkyrinParvinMutagenesisMammals
2008
The structural basis of integrin-linked kinase–PINCH interactions
Chiswell BP, Zhang R, Murphy JW, Boggon TJ, Calderwood DA. The structural basis of integrin-linked kinase–PINCH interactions. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 20677-20682. PMID: 19074270, PMCID: PMC2634877, DOI: 10.1073/pnas.0811415106.Peer-Reviewed Original ResearchConceptsIntegrin-linked kinaseLIM1 domainGrowth factor signalingAtomic resolution descriptionILK bindingAnkyrin repeatsILK-PINCHHeterotrimeric complexZinc fingerMolecular basisMutagenesis dataStructural basisCell adhesionPoint mutationsConformational flexibilityKey interactionsParvinConvergence pointLim1DomainAnkyrinKinaseComplexesRepeatsSignaling
2003
Structural Determinants of Integrin Recognition by Talin
Garcı́a-Alvarez B, de Pereda JM, Calderwood DA, Ulmer TS, Critchley D, Campbell ID, Ginsberg MH, Liddington RC. Structural Determinants of Integrin Recognition by Talin. Molecular Cell 2003, 11: 49-58. PMID: 12535520, DOI: 10.1016/s1097-2765(02)00823-7.Peer-Reviewed Original ResearchConceptsBidirectional signal transductionFragment of talinIntegrin adhesion receptorsFERM domainIntegrin tailsCytoplasmic domainCytoplasmic proteinsSignal transductionIntegrin linkagesTransmembrane receptorsTalinMutational analysisAdhesion receptorsDomain recognitionCell interiorIntegrin recognitionStructural determinantsLigand interactionsNovel variantsStructural paradigmFragmentsTransductionReceptorsTailDomain
2000
Distinct Domains of CD98hc Regulate Integrins and Amino Acid Transport*
Fenczik C, Zent R, Dellos M, Calderwood D, Satriano J, Kelly C, Ginsberg M. Distinct Domains of CD98hc Regulate Integrins and Amino Acid Transport*. Journal Of Biological Chemistry 2000, 276: 8746-8752. PMID: 11121428, DOI: 10.1074/jbc.m011239200.Peer-Reviewed Original ResearchConceptsAmino acid transportIntegrin functionAcid transportDistinct domainsType II transmembrane proteinIsoleucine transportAmino acid transportersCD98 heavy chainCell surface heterodimersTransmembrane domainCytoplasmic domainTransmembrane proteinSurface heterodimersExtracellular domainAcid transportersCD98hcHeavy chainProteinIntegrinsCovalent linkageDifferent light chainsLight chainDomainMutantsHeterodimersClass- and Splice Variant-specific Association of CD98 with Integrin β Cytoplasmic Domains*
Zent R, Fenczik C, Calderwood D, Liu S, Dellos M, Ginsberg M. Class- and Splice Variant-specific Association of CD98 with Integrin β Cytoplasmic Domains*. Journal Of Biological Chemistry 2000, 275: 5059-5064. PMID: 10671548, DOI: 10.1074/jbc.275.7.5059.Peer-Reviewed Original ResearchConceptsCytoplasmic domainIntegrin activationMuscle-specific splice variantIntegrin beta cytoplasmic domainsBasic amino acid transportType II transmembrane proteinIntegrin β cytoplasmic domainBeta cytoplasmic domainsIntegrin cytoplasmic domainCell fusion eventsIntegrin adhesion receptorsAmino acid transportTransmembrane proteinMembrane proteinsFusion eventsIntegrin classAdhesion receptorsSplice variantsAcid transportCD98Variant specificityProteinIntegrinsDomainActivation