2005
Characterization of in vivo expanded OspA-specific human T-cell clones
Ausubel LJ, O'Connor KC, Baecher-Allen C, Trollmo C, Kessler B, Hekking B, Merritt D, Meyer AL, Kwok B, Ploegh H, Huber BT, Hafler DA. Characterization of in vivo expanded OspA-specific human T-cell clones. Clinical Immunology 2005, 115: 313-322. PMID: 15893699, DOI: 10.1016/j.clim.2005.02.015.Peer-Reviewed Original ResearchConceptsT cell clonesMajor histocompatibility complex class II tetramersTreatment-resistant Lyme arthritisCD4 T-cell clonesDistinct T-cell clonesT cell receptor repertoireHuman T cell clonesClass II tetramersBeta chainT cell recognitionTCR contact residuesTCR beta chainT cell receptorCell flow cytometryTCR usageImmune compartmentLyme arthritisAutoimmune diseasesMicrobial antigensT cellsOspA epitopeImmunodominant epitopesSynovial fluidReceptor repertoireReactive clones
2004
PD-1 ligands, negative regulators for activation of naïve, memory, and recently activated human CD4+ T cells
Cai G, Karni A, Oliveira EM, Weiner HL, Hafler DA, Freeman GJ. PD-1 ligands, negative regulators for activation of naïve, memory, and recently activated human CD4+ T cells. Cellular Immunology 2004, 230: 89-98. PMID: 15598424, DOI: 10.1016/j.cellimm.2004.09.004.Peer-Reviewed Original ResearchConceptsPD-1 ligand blockadeT cellsPD-L1Myelin basic proteinPD-L2Human CD4Ex vivo dendritic cellsVivo dendritic cellsPD-1 ligandsPD-1 pathwayPrimary responseActivation of naïveNaive T cellsPD-1Dendritic cellsCytokine productionNormal donorsIFN-gammaSecondary responseCD4BlockadeHigh expressionNegative regulatory pathwaysInduced activationBasic protein
2001
Molecular Mimicry in Lyme Arthritis Demonstrated at the Single Cell Level: LFA-1αL Is a Partial Agonist for Outer Surface Protein A-Reactive T Cells
Trollmo C, Meyer A, Steere A, Hafler D, Huber B. Molecular Mimicry in Lyme Arthritis Demonstrated at the Single Cell Level: LFA-1αL Is a Partial Agonist for Outer Surface Protein A-Reactive T Cells. The Journal Of Immunology 2001, 166: 5286-5291. PMID: 11290815, DOI: 10.4049/jimmunol.166.8.5286.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferi GroupClone CellsHumansHybridomasLipoproteinsLyme DiseaseLyme Disease VaccinesLymphocyte ActivationLymphocyte Function-Associated Antigen-1MiceMice, TransgenicMolecular MimicryPeptide FragmentsT-Lymphocyte SubsetsConceptsIL-13Antibiotic treatment-resistant Lyme arthritisPartial agonistTreatment-resistant Lyme arthritisChronic inflammatory joint diseaseT cell hybridsDR4 transgenic miceHuman T cell clonesClass II tetramersInflammatory joint diseaseSymptoms of arthritisT cell responsesT cell levelsEpisodes of arthritisT cell clonesSurface protein AAutoimmune mechanismsOuter surface protein ALyme arthritisJoint diseaseT cellsIFN-gammaImmunodominant epitopesCell levelTransgenic mice
2000
Direct enumeration of Borrelia-reactive CD4 T cells ex vivo by using MHC class II tetramers
Meyer A, Trollmo C, Crawford F, Marrack P, Steere A, Huber B, Kappler J, Hafler D. Direct enumeration of Borrelia-reactive CD4 T cells ex vivo by using MHC class II tetramers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 11433-11438. PMID: 11005833, PMCID: PMC17217, DOI: 10.1073/pnas.190335897.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBiopolymersBorrelia burgdorferi GroupCD4-Positive T-LymphocytesClone CellsCytokinesDose-Response Relationship, ImmunologicHLA-DR AntigensHLA-DRB1 ChainsHumansIn Vitro TechniquesLipoproteinsLyme DiseaseLyme Disease VaccinesConceptsClass II tetramersMHC class II tetramersT cellsSynovial fluidPeripheral bloodMajor histocompatibility complex class II tetramersTreatment-resistant Lyme arthritisAntigen-reactive T cellsCD4 T cellsDifferent cytokine profilesIL-13 secretionT cell clonesAllogeneic feeder cellsCytokine profileLyme arthritisInflammatory compartmentIL-2IFN-gammaImmunodominant epitopesCell clonesBorrelia burgdorferiPatientsHLABloodCells
1992
CTLA-4 and CD28 mRNA are coexpressed in most T cells after activation. Expression of CTLA-4 and CD28 mRNA does not correlate with the pattern of lymphokine production.
Freeman GJ, Lombard DB, Gimmi CD, Brod SA, Lee K, Laning JC, Hafler DA, Dorf ME, Gray GS, Reiser H. CTLA-4 and CD28 mRNA are coexpressed in most T cells after activation. Expression of CTLA-4 and CD28 mRNA does not correlate with the pattern of lymphokine production. The Journal Of Immunology 1992, 149: 3795-801. PMID: 1281186, DOI: 10.4049/jimmunol.149.12.3795.Peer-Reviewed Original ResearchMeSH KeywordsAbataceptAnimalsAntigens, CDAntigens, DifferentiationAntigens, Differentiation, T-LymphocyteAntigens, SurfaceB7-1 AntigenBase SequenceBlotting, NorthernCD28 AntigensCell Adhesion MoleculesCell LineCTLA-4 AntigenHumansImmunoconjugatesInterferon-gammaInterleukinsLeukemia, T-CellLymphocyte ActivationLymphokinesMiceMolecular Sequence DataOligonucleotide ProbesPolymerase Chain ReactionRNA, MessengerT-LymphocytesTumor Necrosis Factor-alphaConceptsT cell clonesCTLA-4 mRNACTLA-4T cellsActivated T cellsT cell activationT cell linesMurine T cell clonesCell clonesCD28 mRNACostimulatory signalsT cell receptor-dependent stimulationCell activationNormal T cell subsetsAg-presenting cellsHuman T cell clonesT cell subsetsExpression of CD28Th2 cytokine profileMost T cellsLeukemic T cell lineCell linesReceptor-dependent stimulationSuch costimulatory signalsInteraction of B7
1991
Presentation of autoantigen by human T cells.
LaSalle JM, Ota K, Hafler DA. Presentation of autoantigen by human T cells. The Journal Of Immunology 1991, 147: 774-80. PMID: 1713605, DOI: 10.4049/jimmunol.147.3.774.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, CDAntigens, Differentiation, T-LymphocyteAntigens, SurfaceAutoantigensCD2 AntigensCD58 AntigensEpitopesHistocompatibility AntigensHLA-D AntigensHumansIntegrin beta1Leukocyte Common AntigensLymphocyte ActivationLymphocyte Function-Associated Antigen-1Membrane GlycoproteinsMolecular Sequence DataMyelin Basic ProteinReceptors, ImmunologicT-LymphocytesConceptsT cell clonesMBP-reactive T cell clonesAutologous T cellsMyelin basic proteinMHC class IIT cellsHuman T cellsCell clonesAdhesion molecules LFA-3Class IIReactive T cell clonesWhole myelin basic proteinPresentation of autoantigensT cell interactionsActivated T cellsT cell presentationTCR-mediated responsesAg preparationsImmune responseMHC classSelf-AgForeign AgsInflammatory sitesCell presentationLFA-3
1990
T‐T cell interactions are mediated by adhesion molecules
Brod S, Purvee M, Benjamin D, Hafler D. T‐T cell interactions are mediated by adhesion molecules. European Journal Of Immunology 1990, 20: 2259-2268. PMID: 1700751, DOI: 10.1002/eji.1830201015.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigens, Differentiation, T-LymphocyteAntigens, SurfaceCD3 ComplexCD58 AntigensCell Adhesion MoleculesCell CommunicationClone CellsHumansImmunophenotypingInterleukin-2Lymphocyte ActivationLymphocyte Function-Associated Antigen-1Membrane GlycoproteinsMitogensReceptors, Antigen, T-CellT-LymphocytesConceptsT cell clonesAutologous T cell clonesPeripheral blood T cellsAdhesion molecules LFA-1Blood T cellsT cellsLFA-3Major histocompatibility complexInflammatory responseCell clonesICAM-1LFA-1T cell immune functionCD3/T cell receptor complexSpecific T cellsCell adhesion molecules LFA-1Cell immune functionT cell interactionsT cell activationT cell receptor complexMHC determinantsCell receptor complexImmune functionCell activationCD2 pathway
1989
Mechanisms of immune memory. T cell activation and CD3 phosphorylation correlates with Ta1 (CDw26) expression.
Hafler DA, Chofflon M, Benjamin D, Dang NH, Breitmeyer J. Mechanisms of immune memory. T cell activation and CD3 phosphorylation correlates with Ta1 (CDw26) expression. The Journal Of Immunology 1989, 142: 2590-6. PMID: 2564857, DOI: 10.4049/jimmunol.142.8.2590.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, T-LymphocyteAntigens, SurfaceCalciumCD2 AntigensCD3 ComplexHumansImmunologic MemoryLymphocyte ActivationPhosphorylationProtein Kinase CReceptors, Antigen, T-CellReceptors, ImmunologicT-LymphocytesTetradecanoylphorbol AcetateTumor Necrosis Factor Receptor Superfamily, Member 7ConceptsT cell immune memoryT cell activationImmune memoryT cellsCell activationPeripheral blood T cellsTA1 expressionBlood T cellsIntracellular calcium levelsTA1 cellsCD3/TCRT cell linesCalcium ionophore ionomycinAg challengeIL-2T lymphocytesMitogenic antibodiesCalcium levelsPhorbol ester PMAIonophore ionomycinCD2 moleculeGrowth factorActivation requirementsInvariant chainCD2 stimulation
1987
Secondary immune amplification following live poliovirus immunization in humans
Hafler D, Fox D, Benjamin D, Blue M, Weiner H. Secondary immune amplification following live poliovirus immunization in humans. Clinical Immunology 1987, 44: 321-328. PMID: 3040309, DOI: 10.1016/0090-1229(87)90076-6.Peer-Reviewed Original ResearchConceptsAutologous mixed lymphocyte responseRecall antigensAnamnestic responseImmune amplificationImmune responseHuman T cell responsesT cell proliferative responsesLive virus infectionT cell responsesMixed lymphocyte responseSecondary immune responsePoliovirus immunizationLymphocyte responsesVirus infectionProliferative responsePoliovirus infectionAntigenPotential mechanismsAmplification pathwayConsistent changesImmunizationInfectionPoliovirusSubjectsResponseMyelin basic protein and proteolipid protein reactivity of brain- and cerebrospinal fluid-derived T cell clones in multiple sclerosis and postinfectious encephalomyelitis.
Hafler DA, Benjamin DS, Burks J, Weiner HL. Myelin basic protein and proteolipid protein reactivity of brain- and cerebrospinal fluid-derived T cell clones in multiple sclerosis and postinfectious encephalomyelitis. The Journal Of Immunology 1987, 139: 68-72. PMID: 2438352, DOI: 10.4049/jimmunol.139.1.68.Peer-Reviewed Original ResearchConceptsT cell clonesMyelin basic proteinPeripheral bloodPostinfectious encephalomyelitisCell clonesMultiple sclerosisT cellsProteolipid proteinPeripheral blood T cell clonesBlood T-cell clonePlaque tissueMS plaque tissueProportion of T4T cell reactivityHuman myelin basic proteinBasic proteinMS patientsCell reactivityIL-2Cerebrospinal fluidTarget antigenClear reactivityBrain tissueBloodEncephalomyelitis
1986
Antigen reactive memory T cells are defined by Ta1.
Hafler DA, Fox DA, Benjamin D, Weiner HL. Antigen reactive memory T cells are defined by Ta1. The Journal Of Immunology 1986, 137: 414-8. PMID: 3013990, DOI: 10.4049/jimmunol.137.2.414.Peer-Reviewed Original ResearchConceptsT cellsReactive memory T cellsPeripheral blood T cellsAutologous mixed lymphocyte responseHuman peripheral blood T cellsMemory T cellsMixed lymphocyte responseBlood T cellsPresence of PWMT cell clonesEnhanced proliferative responseTA1 cellsActivated T cellsNormal human subjectsT cell linesT memory cellsSuppressor cellsLymphocyte responsesAnamnestic responseMitogen PHAPeripheral bloodLymphocyte populationsIL-2Tetanus toxoidT lymphocytes
1985
Investigation of in vivo activated T cells in multiple sclerosis and inflammatory central nervous system diseases
Hafler DA, Hemler ME, Christenson L, Williams JM, Shapiro HM, Strom TB, Strominger JL, Weiner HL. Investigation of in vivo activated T cells in multiple sclerosis and inflammatory central nervous system diseases. Clinical Immunology 1985, 37: 163-171. PMID: 3930113, DOI: 10.1016/0090-1229(85)90147-3.Peer-Reviewed Original ResearchConceptsT cellsActivated T cellsActivation antigensMultiple sclerosisInflammatory central nervous system diseasesPeripheral blood T cellsCentral nervous system diseaseActive multiple sclerosisSystemic immune activationGroup of patientsMultiple sclerosis patientsBlood T cellsEarly activation antigenNervous system diseasesAbnormal immunologic activityMultiple differentiation stagesMS patientsSclerosis patientsViral encephalitisImmune activationPeripheral bloodNeurologic diseaseImmunologic activitySystem diseasesNormal controlsIn Vivo Activated T Lymphocytes in the Peripheral Blood and Cerebrospinal Fluid of Patients with Multiple Sclerosis
Hafler D, Fox D, Manning M, Schlossman S, Reinherz E, Weiner H. In Vivo Activated T Lymphocytes in the Peripheral Blood and Cerebrospinal Fluid of Patients with Multiple Sclerosis. New England Journal Of Medicine 1985, 312: 1405-1411. PMID: 2985995, DOI: 10.1056/nejm198505303122201.Peer-Reviewed Original ResearchConceptsProgressive multiple sclerosisMultiple sclerosisInterleukin-2 receptorNervous system diseasesNeurologic diseaseSpinal fluidPeripheral bloodSystem diseasesCerebrospinal fluidInflammatory central nervous system diseasesCentral nervous system diseaseSystemic immune activationInflammatory neurologic diseasesPeripheral blood lymphocytesBlood of patientsActivated T lymphocytesAbnormal immunologic activityCent of lymphocytesCent of cellsT cell activationImmune activationT cellsT lymphocytesImmunologic activityNormal controls