2014
Preanalytical variables and phosphoepitope expression in FFPE tissue: quantitative epitope assessment after variable cold ischemic time
Vassilakopoulou M, Parisi F, Siddiqui S, England AM, Zarella ER, Anagnostou V, Kluger Y, Hicks DG, Rimm DL, Neumeister VM. Preanalytical variables and phosphoepitope expression in FFPE tissue: quantitative epitope assessment after variable cold ischemic time. Laboratory Investigation 2014, 95: 334-341. PMID: 25418580, DOI: 10.1038/labinvest.2014.139.Peer-Reviewed Original ResearchConceptsCold ischemic timeIschemic timeFormalin fixationCompanion diagnostic testingCancer patientsBreast cancerTissue microarrayPhospho-HSP27Breast tumorsDiagnostic testingQuantitative immunofluorescenceAQUA technologyPreanalytical variablesGene expression profilingLoss of antigenicityTissue samplesReproducible assessmentProtein levelsConfidence intervalsSpecimen collectionExpression levelsPhosphorylation levelsAntigenicityRoutine usageResearch settingsERβ splice variant expression in four large cohorts of human breast cancer patient tumors
Wimberly H, Han G, Pinnaduwage D, Murphy LC, Yang XR, Andrulis IL, Sherman M, Figueroa J, Rimm DL. ERβ splice variant expression in four large cohorts of human breast cancer patient tumors. Breast Cancer Research And Treatment 2014, 146: 657-667. PMID: 25007965, PMCID: PMC6939385, DOI: 10.1007/s10549-014-3050-3.Peer-Reviewed Original ResearchConceptsBreast cancerPatient tumorsERβ variantsQuantitative immunofluorescenceBreast cancer patient tumorsTriple-negative patientsBreast cancer outcomesAbsence of ERαBreast cancer biologyCancer patient tumorsBreast cancer patient samplesSplice variant expressionCancer patient samplesParaffin-embedded tissuesQIF scoresCancer outcomesPredictive biomarkersWorse outcomesEstrogen receptorLarge cohortSurvival analysisERβPatient samplesAQUA technologyVariant expression
2011
Standardization of Epidermal Growth Factor Receptor (EGFR) Measurement by Quantitative Immunofluorescence and Impact on Antibody-Based Mutation Detection in Non–Small Cell Lung Cancer
Dimou A, Agarwal S, Anagnostou V, Viray H, Christensen S, Rothberg B, Zolota V, Syrigos K, Rimm DL. Standardization of Epidermal Growth Factor Receptor (EGFR) Measurement by Quantitative Immunofluorescence and Impact on Antibody-Based Mutation Detection in Non–Small Cell Lung Cancer. American Journal Of Pathology 2011, 179: 580-589. PMID: 21722621, PMCID: PMC3157192, DOI: 10.1016/j.ajpath.2011.04.031.Peer-Reviewed Original ResearchConceptsNon-small cell lung cancerCell lung cancerPrognostic valueMutation-specific antibodiesLung cancerQuantitative immunofluorescenceEpidermal growth factor receptor (EGFR) protein expressionIndependent NSCLC cohortsPopulation-based cohortEGFR mutation rateReceptor protein expressionTotal proteinFalse-positive casesPrediction of responseWestern blot analysisNSCLC cohortRetrospective cohortReceptor measurementsYale cohortEGFR expressionCohortEGFRAQUA technologyProtein expressionAntibodies
2010
Subcellular Localization of NEDD9 AND HMB45 with AQUA Technology To Distinguish Spitz Nevi from Melanoma
McRae M, Lasova R, Narayan D, Rimm D. Subcellular Localization of NEDD9 AND HMB45 with AQUA Technology To Distinguish Spitz Nevi from Melanoma. Plastic & Reconstructive Surgery 2010, 126: 130-131. DOI: 10.1097/01.prs.0000388855.02380.53.Peer-Reviewed Original ResearchAQUA technologySpitz nevi
2009
Preliminary Comparison between AQUA and Centralised ER/PgR Analysis within the TEAM Pathology Study.
Bartlett J, Rimm D, Brookes C, Dolled-Filhart M, Robson T, van de Velde C, Billingham L, Campbell F, Hasenburg A, Hille E, Kieback D, Putter H, Markopoulos C, Christiansen J, Gustavson M, Mallon E, Meershoek-Klein Kranenburg E, Parideans R, Seynaeve C, Rea D. Preliminary Comparison between AQUA and Centralised ER/PgR Analysis within the TEAM Pathology Study. Cancer Research 2009, 69: 3045-3045. DOI: 10.1158/0008-5472.sabcs-09-3045.Peer-Reviewed Original ResearchER/PgRER expressionImmunohistochemical assaysAQUA scoreExemestane Adjuvant Multinational (TEAM) trialHigh ER expressionTeam patientsPatient prognosisAromatase inhibitorsMultinational trialOutcome dataPathology studiesCancer ResAQUA technologyIHCPreliminary dataPatientsRegression analysisPGR analysisFurther dataPgRImage analysisIncremental increaseScoresMultiple coresEvaluation of the false-negative rate of standardized and quantitative measurement of estrogen receptor (ER) in tissue using AQUA technology
Gustavson M, Welsh A, Jones C, Mayotte J, Tu J, Hopkins J, Rimm D, Christiansen J. Evaluation of the false-negative rate of standardized and quantitative measurement of estrogen receptor (ER) in tissue using AQUA technology. Journal Of Clinical Oncology 2009, 27: 567-567. DOI: 10.1200/jco.2009.27.15_suppl.567.Peer-Reviewed Original ResearchER protein expressionEstrogen receptorBreast cancer samplesPg/AQUA analysisFalse negative rateAQUA technologyCancer samplesAllred scoring systemProtein expressionChange of diagnosisEstrogen receptor testingReproducible diagnostic testFalse-negative testsHigh false-negative rateReceptor testingER testingAllred scoreLarge cohortNegative testAQUA scoreScoring systemMisclassified casesClinical settingConcordance rate