Standardization of Estrogen Receptor Measurement in Breast Cancer Suggests False-Negative Results Are a Function of Threshold Intensity Rather Than Percentage of Positive Cells
Welsh AW, Moeder CB, Kumar S, Gershkovich P, Alarid ET, Harigopal M, Haffty BG, Rimm DL. Standardization of Estrogen Receptor Measurement in Breast Cancer Suggests False-Negative Results Are a Function of Threshold Intensity Rather Than Percentage of Positive Cells. Journal Of Clinical Oncology 2011, 29: 2978-2984. PMID: 21709197, PMCID: PMC3157961, DOI: 10.1200/jco.2010.32.9706.Peer-Reviewed Original ResearchConceptsER-positive patientsEstrogen receptorQuantitative immunofluorescenceBreast cancerTissue microarrayPositive cellsIndependent retrospective cohortsEstrogen receptor measurementsAssessment of survivalTMA cohortFalse-negative resultsRetrospective cohortER immunoreactivityTest discordancePrognostic outcomesIndependent cohortReceptor measurementsLimitations of immunohistochemistryPatientsDiscrepant casesCohortIHC methodPathologists' judgmentsDiscrepant resultsStandardized assaysStandardization of Epidermal Growth Factor Receptor (EGFR) Measurement by Quantitative Immunofluorescence and Impact on Antibody-Based Mutation Detection in Non–Small Cell Lung Cancer
Dimou A, Agarwal S, Anagnostou V, Viray H, Christensen S, Rothberg B, Zolota V, Syrigos K, Rimm DL. Standardization of Epidermal Growth Factor Receptor (EGFR) Measurement by Quantitative Immunofluorescence and Impact on Antibody-Based Mutation Detection in Non–Small Cell Lung Cancer. American Journal Of Pathology 2011, 179: 580-589. PMID: 21722621, PMCID: PMC3157192, DOI: 10.1016/j.ajpath.2011.04.031.Peer-Reviewed Original ResearchConceptsNon-small cell lung cancerCell lung cancerPrognostic valueMutation-specific antibodiesLung cancerQuantitative immunofluorescenceEpidermal growth factor receptor (EGFR) protein expressionIndependent NSCLC cohortsPopulation-based cohortEGFR mutation rateReceptor protein expressionTotal proteinFalse-positive casesPrediction of responseWestern blot analysisNSCLC cohortRetrospective cohortReceptor measurementsYale cohortEGFR expressionCohortEGFRAQUA technologyProtein expressionAntibodies