Multiplex Immunohistochemistry and Immunofluorescence: A Practical Update for Pathologists
Harms P, Frankel T, Moutafi M, Rao A, Rimm D, Taube J, Thomas D, Chan M, Pantanowitz L. Multiplex Immunohistochemistry and Immunofluorescence: A Practical Update for Pathologists. Modern Pathology 2023, 36: 100197. PMID: 37105494, DOI: 10.1016/j.modpat.2023.100197.Peer-Reviewed Original ResearchConceptsDigital spatial profilingMost clinical laboratoriesTumor microenvironmentImmunofluorescence/immunohistochemistryImmuno-oncology researchDiagnostic practiceClinical laboratoriesRoutine diagnostic useAntitumor immunityAdvanced cancerImmune populationsMultiplex stainingEosin stainingIHC stainsPractical updateClinical diagnostic practiceMultiplex immunohistochemistrySingle biomarkerImmunohistochemistryMultiplexed immunohistochemistryStandardized protocolChromogenic immunohistochemistryDiagnostic useMultiple biomarkersSerial sections