2015
Mapping and Quantitation of the Interaction between the Recombination Activating Gene Proteins RAG1 and RAG2* ♦
Zhang YH, Shetty K, Surleac MD, Petrescu AJ, Schatz DG. Mapping and Quantitation of the Interaction between the Recombination Activating Gene Proteins RAG1 and RAG2* ♦. Journal Of Biological Chemistry 2015, 290: 11802-11817. PMID: 25745109, PMCID: PMC4424321, DOI: 10.1074/jbc.m115.638627.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCatalytic DomainDNA-Binding ProteinsGene Expression RegulationGenome, HumanHEK293 CellsHomeodomain ProteinsHumansInterferometryMaleMiceMice, Inbred C57BLMolecular Sequence DataMutationNuclear ProteinsProtein BindingProtein Interaction MappingProtein Structure, SecondaryThymus GlandV(D)J RecombinationVDJ RecombinasesConceptsRegion of RAG1Α-helixZinc finger regionResidues N-terminalActive siteAcidic amino acidsPulldown assaysAccessory factorsHermes transposaseProteins RAG1Finger regionRAG activityQuantitative Western blottingC-terminusRAG endonucleaseN-terminalCatalytic functionRAG1Amino acidsDNA cleavageRAG2Nuclear concentrationRecombination activityCatalytic centerBiolayer interferometry
2002
Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombination
Tsai CL, Drejer AH, Schatz DG. Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombination. Genes & Development 2002, 16: 1934-1949. PMID: 12154124, PMCID: PMC186421, DOI: 10.1101/gad.984502.Peer-Reviewed Original ResearchAlanineAmino Acid SubstitutionAnimalsCell LineCysteineDNADNA NucleotidyltransferasesDNA-Binding ProteinsGene Rearrangement, B-LymphocyteGenes, RAG-1Glutamic AcidHomeodomain ProteinsHumansMacromolecular SubstancesMiceMutagenesis, Site-DirectedNuclear ProteinsNucleic Acid ConformationPhenotypeProtein Interaction MappingRecombinant Fusion ProteinsRecombination, GeneticRegulatory Sequences, Nucleic AcidSerineSubstrate SpecificityVDJ Recombinases