2022
Prenatal Somatic Cell Gene Therapies: Charting a Path Toward Clinical Applications (Proceedings of the CERSI‐FDA Meeting)
Herzeg A, Almeida‐Porada G, Charo RA, David AL, Gonzalez‐Velez J, Gupta N, Lapteva L, Lianoglou B, Peranteau W, Porada C, Sanders SJ, Sparks TN, Stitelman DH, Struble E, Sumner CJ, MacKenzie TC. Prenatal Somatic Cell Gene Therapies: Charting a Path Toward Clinical Applications (Proceedings of the CERSI‐FDA Meeting). The Journal Of Clinical Pharmacology 2022, 62: s36-s52. PMID: 36106778, PMCID: PMC9547535, DOI: 10.1002/jcph.2127.Peer-Reviewed Original Research
2021
Diffusion weighted imaging as a biomarker of retinoic acid induced myelomeningocele
Maassel N, Farrelly J, Coman D, Freedman-Weiss M, Ahle S, Ullrich S, Yung N, Hyder F, Stitelman D. Diffusion weighted imaging as a biomarker of retinoic acid induced myelomeningocele. PLOS ONE 2021, 16: e0253583. PMID: 34191842, PMCID: PMC8244849, DOI: 10.1371/journal.pone.0253583.Peer-Reviewed Original ResearchConceptsMagnetic resonance imaging techniquesSpinal cordRat fetusesRetinoic acidCommon congenital anomalyComplementary imaging biomarkerLack of skinNeural tube defectsTrans retinoic acidBony coveringRat damsCongenital anomaliesRat modelExperimental therapiesHistopathologic analysisSevere formBrain volumeMyelomeningoceleImaging biomarkersDisease severityMMC defectsTube defectsIncomplete closureFetusesFractional anisotropy (FA) mapsEngineering alginate microparticles for optimized accumulation in fetal rat myelomeningocele
Freedman-Weiss MR, Wu D, Maassel N, Ullrich SJ, Ahle SL, Roberts K, Farrelly JS, Buzzelli G, Saltzman WM, Stitelman DH. Engineering alginate microparticles for optimized accumulation in fetal rat myelomeningocele. Journal Of Pediatric Surgery 2021, 57: 544-550. PMID: 33933264, DOI: 10.1016/j.jpedsurg.2021.03.060.Peer-Reviewed Original ResearchThe parental journey of fetal care: a systematic review and metasynthesis
Wilpers A, Bahtiyar MO, Stitelman D, Batten J, Calix RX, Chase V, Yung N, Maassel N, Novick G. The parental journey of fetal care: a systematic review and metasynthesis. American Journal Of Obstetrics & Gynecology MFM 2021, 3: 100320. PMID: 33493706, DOI: 10.1016/j.ajogmf.2021.100320.Peer-Reviewed Original ResearchNanoparticles for delivery of agents to fetal lungs
Ullrich SJ, Freedman-Weiss M, Ahle S, Mandl HK, Piotrowski-Daspit AS, Roberts K, Yung N, Maassel N, Bauer-Pisani T, Ricciardi AS, Egan ME, Glazer PM, Saltzman WM, Stitelman DH. Nanoparticles for delivery of agents to fetal lungs. Acta Biomaterialia 2021, 123: 346-353. PMID: 33484911, PMCID: PMC7962939, DOI: 10.1016/j.actbio.2021.01.024.Peer-Reviewed Original ResearchConceptsFetal lungCellular uptakeIntra-amniotic routeRoute of deliveryCongenital lung diseaseDelivery of agentsIntra-amniotic deliveryRelative cellular uptakeNanoparticlesFetal treatmentDiaphragmatic herniaLung diseaseFetal therapyLung tissueFetal miceIntravenous deliveryCystic fibrosisLungLung therapyInterventional technologiesTherapeutic agentsEndothelial cellsCell populationsEffective targetingTherapy
2020
The in-utero diagnosis of choledochal cyst: can postnatal imaging predict benefit from early surgical intervention?
Cochran ED, Lazow SP, Kim AG, Burkhalter LS, Frost NW, Stitelman D, Davis J, Santiago-Munoz P, Buchmiller TL, Perrone EE, Schindel DT. The in-utero diagnosis of choledochal cyst: can postnatal imaging predict benefit from early surgical intervention? The Journal Of Maternal-Fetal & Neonatal Medicine 2020, 35: 1070-1074. PMID: 32188329, DOI: 10.1080/14767058.2020.1742320.Peer-Reviewed Original ResearchConceptsInitial postnatal ultrasoundCholedochal cystCyst sizeEarly surgical interventionEarly complicationsCyst excisionClinical courseFetal centerMedian ageCyst resolutionPostnatal ultrasoundSurgical interventionPrenatal suspicionIRB approvalPatientsUtero diagnosisDiagnostic revisionFirst monthSymptom manifestationCystsNewbornsBirthFirst yearUltrasoundSymptom development
2019
An Evidence-Based Guideline Supporting Restricted Opioid Prescription after Pediatric Appendectomy
Freedman-Weiss MR, Chiu AS, Worhunsky D, Manchisi A, Torres-Maldonado I, Sagnella L, Caty MG, Cowles RA, Ozgediz DE, Christison-Lagay ER, Solomon DG, Stitelman DH. An Evidence-Based Guideline Supporting Restricted Opioid Prescription after Pediatric Appendectomy. Journal Of Pediatric Surgery 2019, 55: 106-111. PMID: 31699433, DOI: 10.1016/j.jpedsurg.2019.09.063.Peer-Reviewed Original ResearchConceptsLaparoscopic appendectomyOpioid prescriptionsPostoperative opioid needsPostoperative telephone interviewsQuantity of opioidsPresence of painYoung adult patientsEvidence-based guidelinesMain outcome measuresOpioid needsAnalgesic useModifiable contributorPain reliefAdequate analgesiaAdult patientsChart reviewHospital dischargeMost patientsPatients 5Pediatric appendectomyYounger patientsPain concernsAverage MMEOpioid epidemicOutcome measuresUtility of Fetal Cardiac Axis and Cardiac Position Assessment in Predicting Neonatal Respiratory Morbidity in Fetal Congenital Lung Lesions
Tuzovic L, Copel JA, Stitelman DH, Levit O, Bahtiyar MO. Utility of Fetal Cardiac Axis and Cardiac Position Assessment in Predicting Neonatal Respiratory Morbidity in Fetal Congenital Lung Lesions. Journal Of Ultrasound In Medicine 2019, 38: 2361-2372. PMID: 30653685, DOI: 10.1002/jum.14931.Peer-Reviewed Original ResearchConceptsAbnormal cardiac axisNegative predictive valueFetal cardiac axisNeonatal respiratory morbidityRespiratory morbidityCongenital lung lesionsCardiac positionPositive predictive valuePredictive valueCardiac axisLung lesionsHigh negative predictive valueSevere respiratory morbidityRetrospective cohort studyRight-sided lesionsNormal cardiac axisCohort studyWeeks' gestationMorbidityCP assessmentWeeksDiagnostic performanceLesionsPatientsGestation
2018
Opioid Prescribing Habits of General Versus Pediatric Surgeons After Uncomplicated Laparoscopic Appendectomy
Freedman-Weiss MR, Chiu A, Solomon DG, Christison-Lagay ER, Ozgediz DE, Cowles RA, Caty MG, Stitelman DH. Opioid Prescribing Habits of General Versus Pediatric Surgeons After Uncomplicated Laparoscopic Appendectomy. Journal Of Surgical Research 2018, 235: 404-409. PMID: 30691822, DOI: 10.1016/j.jss.2018.09.085.Peer-Reviewed Original ResearchConceptsOral morphine equivalentsUncomplicated laparoscopic appendectomyAmount of opioidsLaparoscopic appendectomyGeneral surgeonsPediatric surgeonsSurgery providersFuture drug abuseOpioid prescribing habitsPills of oxycodoneRetrospective chart reviewYoung adult patientsPrimary outcome measureType of analgesiaGeneral surgery servicePediatric laparoscopic appendectomyHigh-risk populationLength of stayCommon surgical procedureSingle health systemAge group 13More opioidsMorphine equivalentsModifiable contributorOpioid analgesiaRoutine postnatal chest x-ray and intensive care admission are unnecessary for a majority of infants with congenital lung malformations
Greig CJ, Keiser AM, Cleary MA, Stitelman DH, Christison-Lagay ER, Ozgediz DE, Solomon DG, Caty MG, Cowles RA. Routine postnatal chest x-ray and intensive care admission are unnecessary for a majority of infants with congenital lung malformations. Journal Of Pediatric Surgery 2018, 54: 670-674. PMID: 30503193, DOI: 10.1016/j.jpedsurg.2018.10.063.Peer-Reviewed Original ResearchConceptsAbnormal chest X-rayCongenital lung malformationsChest X-rayNICU admissionMajority of infantsRespiratory supportBirth weightGestational ageLung malformationsOdds ratioInitial symptomsNeonatal intensive care unit admissionInitial chest X-rayIntensive care unit admissionCare unit admissionIntensive care admissionLevel II evidenceDays of dischargeMajority of casesNICU stayROC cutoffUnit admissionCare admissionFuture care needsImmediate surgeryAlginate microparticles loaded with basic fibroblast growth factor induce tissue coverage in a rat model of myelomeningocele
Farrelly JS, Bianchi AH, Ricciardi AS, Buzzelli GL, Ahle SL, Freedman-Weiss MR, Luks VL, Saltzman WM, Stitelman DH. Alginate microparticles loaded with basic fibroblast growth factor induce tissue coverage in a rat model of myelomeningocele. Journal Of Pediatric Surgery 2018, 54: 80-85. PMID: 30414695, DOI: 10.1016/j.jpedsurg.2018.10.031.Peer-Reviewed Original ResearchConceptsSoft tissue coverageTissue coverageRat modelTime-dated pregnant ratsFree bFGFGrowth factorInjection of microparticlesBasic fibroblast growth factorFibroblast growth factorPregnant ratsPrenatal treatmentIntraamniotic injectionFetal myelomeningoceleMyelomeningoceleMMC coverageMMC defectsRetinoic acidBFGFIn utero nanoparticle delivery for site-specific genome editing
Ricciardi AS, Bahal R, Farrelly JS, Quijano E, Bianchi AH, Luks VL, Putman R, López-Giráldez F, Coşkun S, Song E, Liu Y, Hsieh WC, Ly DH, Stitelman DH, Glazer PM, Saltzman WM. In utero nanoparticle delivery for site-specific genome editing. Nature Communications 2018, 9: 2481. PMID: 29946143, PMCID: PMC6018676, DOI: 10.1038/s41467-018-04894-2.Peer-Reviewed Original ResearchConceptsSite-specific genome editingReversal of splenomegalyPeptide nucleic acidIntra-amniotic administrationBlood hemoglobin levelsMonogenic disordersNanoparticle deliveryPolymeric nanoparticlesPostnatal elevationGestational ageHemoglobin levelsImproved survivalPediatric morbidityDisease improvementHuman β-thalassemiaReticulocyte countNormal organ developmentMouse modelNormal rangeEarly interventionGenome editingOff-target mutationsPostnatal growthGene editingVersatile method
2010
Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral Vector
Stitelman DH, Endo M, Bora A, Muvarak N, Zoltick PW, Flake AW, Brazelton TR. Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral Vector. Molecular Therapy 2010, 18: 1615-1623. PMID: 20571539, PMCID: PMC2956924, DOI: 10.1038/mt.2010.125.Peer-Reviewed Original ResearchConceptsNeural stem cellsAdult neural stem cellsCell typesGene transferStem cellsGreen fluorescent protein marker geneProtein marker geneNervous system developmentNeural cell typesMajor neural cell typesLentiviral vectorsNeural cell populationsMajor cell typesAdult nervous systemGene functionMarker genesEmbryonic day 8Subventricular zoneEntire nervous systemNervous systemVivo transductionCell populationsFuture clinical applicationsGenetic disordersNeural groove