Featured Publications
Identification and validation of urinary CXCL9 as a biomarker for diagnosis of acute interstitial nephritis
Moledina D, Obeid W, Smith R, Rosales I, Sise M, Moeckel G, Kashgarian M, Kuperman M, Campbell K, Lefferts S, Meliambro K, Bitzer M, Perazella M, Luciano R, Pober J, Cantley L, Colvin R, Wilson F, Parikh C. Identification and validation of urinary CXCL9 as a biomarker for diagnosis of acute interstitial nephritis. Journal Of Clinical Investigation 2023, 133: e168950. PMID: 37395276, PMCID: PMC10313360, DOI: 10.1172/jci168950.Peer-Reviewed Original ResearchConceptsUrinary CXCL9External validation cohortValidation cohortControl groupAIN diagnosisDiscovery cohortKidney tissueDiagnostic biomarkersAcute interstitial nephritisCXCL9 mRNA expressionAcute kidney injuryBiopsy-confirmed diagnosisAvailable clinical testsNational InstituteKidney injuryTubulointerstitial nephritisInterstitial nephritisKidney biopsyHistological confirmationHistological diagnosisTreatment optionsLymphocyte chemotaxisCXCL9MRNA expression differencesPatientsUrine TNF-α and IL-9 for clinical diagnosis of acute interstitial nephritis
Moledina DG, Wilson FP, Pober JS, Perazella MA, Singh N, Luciano RL, Obeid W, Lin H, Kuperman M, Moeckel GW, Kashgarian M, Cantley LG, Parikh CR. Urine TNF-α and IL-9 for clinical diagnosis of acute interstitial nephritis. JCI Insight 2019, 4: e127456. PMID: 31092735, PMCID: PMC6542610, DOI: 10.1172/jci.insight.127456.Peer-Reviewed Original ResearchConceptsAcute interstitial nephritisAcute kidney diseasePrebiopsy diagnosisKidney biopsyKidney diseaseIL-9AIN diagnosisUrine TNFInterstitial nephritisSpecific T cell subsetsAcute tubular injuryDiabetic kidney diseaseIL-9 levelsTNF-α levelsT cell subsetsAddition of biomarkersPlasma cytokinesCytokine levelsTubular injuryHighest quartileMultivariable analysisCell subsetsUrinary TNFBlood eosinophilsGlomerular diseaseDevelopment and external validation of a diagnostic model for biopsy-proven acute interstitial nephritis using electronic health record data
Moledina DG, Eadon MT, Calderon F, Yamamoto Y, Shaw M, Perazella MA, Simonov M, Luciano R, Schwantes-An TH, Moeckel G, Kashgarian M, Kuperman M, Obeid W, Cantley LG, Parikh CR, Wilson FP. Development and external validation of a diagnostic model for biopsy-proven acute interstitial nephritis using electronic health record data. Nephrology Dialysis Transplantation 2021, 37: 2214-2222. PMID: 34865148, PMCID: PMC9755995, DOI: 10.1093/ndt/gfab346.Peer-Reviewed Original ResearchConceptsAcute interstitial nephritisInterstitial nephritisUrine biomarkersBiopsy-proven acute interstitial nephritisElectronic health record dataExternal validation cohortTypical clinical featuresBlood urea nitrogenTumor necrosis factorCharacteristic curve analysisHealth record dataExternal validationElectronic health recordsAIN diagnosisModest AUCsSerum creatinineCreatinine ratioKidney biopsyClinical featuresValidation cohortNecrosis factorUnrecognized casesInterleukin-9PatientsUrea nitrogenMortality after acute kidney injury and acute interstitial nephritis in patients prescribed immune checkpoint inhibitor therapy
Baker ML, Yamamoto Y, Perazella MA, Dizman N, Shirali AC, Hafez N, Weinstein J, Simonov M, Testani JM, Kluger HM, Cantley LG, Parikh CR, Wilson FP, Moledina DG. Mortality after acute kidney injury and acute interstitial nephritis in patients prescribed immune checkpoint inhibitor therapy. Journal For ImmunoTherapy Of Cancer 2022, 10: e004421. PMID: 35354588, PMCID: PMC8968986, DOI: 10.1136/jitc-2021-004421.Peer-Reviewed Original ResearchConceptsAcute interstitial nephritisAcute kidney injuryImmune checkpoint inhibitor therapyCheckpoint inhibitor therapyICI therapyKidney injuryInhibitor therapyInterstitial nephritisTime-varying Cox proportional hazards modelsHigher peak serum creatinineSevere acute kidney injuryCancer typesCox proportional hazards modelAssociations of biopsyBaseline laboratory valuesObservational cohort studyPeak serum creatinineFavorable treatment responseProportional hazards modelAKI patientsTherapy initiationCohort studySerum creatinineUnivariable analysisImmune activationUrine interleukin-9 and tumor necrosis factor-α for prognosis of human acute interstitial nephritis
Moledina DG, Wilson FP, Kukova L, Obeid W, Luciano R, Kuperman M, Moeckel GW, Kashgarian M, Perazella MA, Cantley LG, Parikh CR. Urine interleukin-9 and tumor necrosis factor-α for prognosis of human acute interstitial nephritis. Nephrology Dialysis Transplantation 2020, 36: 1851-1858. PMID: 33125471, PMCID: PMC8476079, DOI: 10.1093/ndt/gfaa169.Peer-Reviewed Original ResearchConceptsAcute interstitial nephritisTumor necrosis factorCorticosteroid useIL-9Interstitial nephritisNecrosis factorAcute kidney injuryIL-9 levelsGlomerular filtration rateSubset of patientsCohort of participantsImmunosuppressive therapyTubulointerstitial infiltratesKidney injuryMedian eGFRKidney functionUrine biomarkersInterstitial fibrosisFiltration rateHistological featuresClinical trialsInterleukin-9PatientsEGFRComplete dataThe Association of COVID-19 With Acute Kidney Injury Independent of Severity of Illness: A Multicenter Cohort Study
Moledina DG, Simonov M, Yamamoto Y, Alausa J, Arora T, Biswas A, Cantley LG, Ghazi L, Greenberg JH, Hinchcliff M, Huang C, Mansour SG, Martin M, Peixoto A, Schulz W, Subair L, Testani JM, Ugwuowo U, Young P, Wilson FP. The Association of COVID-19 With Acute Kidney Injury Independent of Severity of Illness: A Multicenter Cohort Study. American Journal Of Kidney Diseases 2021, 77: 490-499.e1. PMID: 33422598, PMCID: PMC7791318, DOI: 10.1053/j.ajkd.2020.12.007.Peer-Reviewed Original ResearchMeSH KeywordsAcute Kidney InjuryAgedCohort StudiesCOVID-19C-Reactive ProteinCreatinineDiureticsFemaleHospital MortalityHumansIntensive Care UnitsLength of StayMaleMiddle AgedProportional Hazards ModelsRenal DialysisRenal Insufficiency, ChronicRespiration, ArtificialRisk FactorsSARS-CoV-2Severity of Illness IndexUnited StatesVasoconstrictor AgentsConceptsAcute kidney injurySARS-CoV-2Cohort studyRisk factorsCOVID-19Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testingTime-updated Cox proportional hazards modelsDialysis-requiring acute kidney injuryYale New Haven Health SystemHigher inflammatory marker levelsMore acute kidney injuryCox proportional hazards modelMulticenter cohort studyHigh rateInflammatory marker levelsTraditional risk factorsProportional hazards modelCoronavirus disease 2019KDIGO criteriaNephrotoxin exposureKidney injuryInjury independentUnivariable analysisNasopharyngeal samplesMarker levelsUrine Uromodulin as a Biomarker of Kidney Tubulointerstitial Fibrosis
Melchinger H, Calderon-Gutierrez F, Obeid W, Xu L, Shaw MM, Luciano RL, Kuperman M, Moeckel GW, Kashgarian M, Wilson FP, Parikh CR, Moledina DG. Urine Uromodulin as a Biomarker of Kidney Tubulointerstitial Fibrosis. Clinical Journal Of The American Society Of Nephrology 2022, 17: 1284-1292. PMID: 35948365, PMCID: PMC9625093, DOI: 10.2215/cjn.04360422.Peer-Reviewed Original ResearchConceptsInterstitial fibrosis/tubular atrophyUrine uromodulinTubular atrophyThick ascending limbUrine albuminSerum creatinineKidney biopsyTubulointerstitial fibrosisMultivariable linear regression modelsTime of biopsyKidney's thick ascending limbAcademic medical centerHuman kidney biopsiesKidney tubulointerstitial fibrosisTubular healthMultivariable analysisHistologic featuresHistologic findingsHistologic changesKidney fibrosisIndependent associationFibrotic modelMultivariable modelMedical CenterMurine model
2024
Early, Individualized Recommendations for Hospitalized Patients With Acute Kidney Injury
Aklilu A, Menez S, Baker M, Brown D, Dircksen K, Dunkley K, Gaviria S, Farrokh S, Faulkner S, Jones C, Kadhim B, Le D, Li F, Makhijani A, Martin M, Moledina D, Coronel-Moreno C, O’Connor K, Shelton K, Shvets K, Srialluri N, Tan J, Testani J, Corona-Villalobos C, Yamamoto Y, Parikh C, Wilson F, Sundararajan A, Wang A, Schretlen C, Singel D, Moss E, Aune F, Iantosca G, Zou G, Chernova I, Bitzel J, Hernandez J, Lindsley J, Dane K, Orias M, Mehta P, Zassman-Isner S, Wen Y, Freeman N. Early, Individualized Recommendations for Hospitalized Patients With Acute Kidney Injury. JAMA 2024, 332 PMID: 39454050, DOI: 10.1001/jama.2024.22718.Peer-Reviewed Original ResearchElectronic health recordsUsual careHealth recordsAction teamsClinicians of patientsHospitalized patientsFinal follow-upIntervention groupHealth systemStudy pharmacistsStudy physiciansMain OutcomesAssociated with adverse outcomesAcute kidney injuryRandomized clinical trialsPrimary outcomeIndividualized recommendationsNew HavenAdverse outcomesAKI detectionCareRhode IslandComposite outcomeFollow-upInterventionB-243 Validating a highly automated, rapid turnaround urine biomarker assay for diagnosis of acute interstitial nephritis
Obeid W, Moledina D, Parikh C. B-243 Validating a highly automated, rapid turnaround urine biomarker assay for diagnosis of acute interstitial nephritis. Clinical Chemistry 2024, 70: hvae106.600. DOI: 10.1093/clinchem/hvae106.600.Peer-Reviewed Original ResearchAcute interstitial nephritisDiagnosis of acute interstitial nephritisTNF-aMesoscale DiscoveryCXCL-9Urinary tumor necrosis factor-alphaInterstitial nephritisUrine biomarker assayTumor necrosis factor-alphaNecrosis factor-alphaSpike-recovery analysisFactor-alphaPotential diagnostic biomarkersLevels of QCBiomarker assaysDiagnostic biomarkersAssay diluentDilution linearityNephritisAverage recoverySpiking volumeHospital settingLigand 9UrineDiagnosisConcordance of Prebiopsy and Postbiopsy Diagnosis in Patients with Suspected Acute Interstitial Nephritis or Acute Tubular Injury
Menez S, Hu D, Philbrook H, Villalobos C, Wen Y, Moledina D, Coca S, Parikh C. Concordance of Prebiopsy and Postbiopsy Diagnosis in Patients with Suspected Acute Interstitial Nephritis or Acute Tubular Injury. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.20248dgm2g87. DOI: 10.1681/asn.20248dgm2g87.Peer-Reviewed Original ResearchBleeding Complications after Native Kidney Biopsy Associated with Aspirin Use
Menez S, Hu D, Philbrook H, Bitzel J, Wen Y, Atta M, Srialluri N, Sperati J, Jaar B, Villalobos C, Shelton K, Makhijani A, Moledina D, Parikh C. Bleeding Complications after Native Kidney Biopsy Associated with Aspirin Use. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.2024stvx1gyn. DOI: 10.1681/asn.2024stvx1gyn.Peer-Reviewed Original ResearchExternal and Temporal Validation of Acute Tubulointerstitial Nephritis (AIN) Diagnostic Index: An Electronic Health Record-Based Multivariable Diagnostic Model for Assessing Probability of Acute Interstitial Nephritis on Kidney Biopsy
Shelton K, Menez S, Shaw M, Kent C, Bitzel J, Philbrook H, Wilson F, Hu D, Wen Y, Parikh C, Moledina D. External and Temporal Validation of Acute Tubulointerstitial Nephritis (AIN) Diagnostic Index: An Electronic Health Record-Based Multivariable Diagnostic Model for Assessing Probability of Acute Interstitial Nephritis on Kidney Biopsy. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.2024z9c9373z. DOI: 10.1681/asn.2024z9c9373z.Peer-Reviewed Original ResearchRisk vs. Reward: Impact of Kidney Biopsies on the Management of Critically Ill Patients
Llama A, Sadarangani S, Moledina D, Luciano R. Risk vs. Reward: Impact of Kidney Biopsies on the Management of Critically Ill Patients. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.2024dd7bnz12. DOI: 10.1681/asn.2024dd7bnz12.Peer-Reviewed Original ResearchUrine Proteomic Signatures of Kidney Function Decline after Hospitalization
Wen Y, Menez S, Philbrook H, Moledina D, Bhatraju P, Coca S, Chinchilli V, Go A, Siew E, Kimmel P, Ikizler T, Hsu C, Himmelfarb J, Cantley L, Parikh C. Urine Proteomic Signatures of Kidney Function Decline after Hospitalization. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.20245v3jz4vr. DOI: 10.1681/asn.20245v3jz4vr.Peer-Reviewed Original ResearchAKI in the Kidney Precision Medicine Project: Acute Tubular Injury vs. Acute Interstitial Nephritis
Schaub J, Castro A, Menez S, Wen Y, Menon R, Bhatraju P, Wilson F, Coca S, Eadon M, Bomback A, Moledina D, Cantley L, Palevsky P, D'Agati V, Randhawa P, Murugan R, Alpers C, Kretzler M, Himmelfarb J, Parikh C. AKI in the Kidney Precision Medicine Project: Acute Tubular Injury vs. Acute Interstitial Nephritis. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.2024cxh8wvky. DOI: 10.1681/asn.2024cxh8wvky.Peer-Reviewed Original ResearchAdjudication of Kidney Biopsy Histopathology to Establish Consensus Diagnosis of Tubulointerstitial Nephritis
Sadarangani S, Shaw M, Moeckel G, Kumar D, Kuperman M, Rosenberg A, Luciano R, Menez S, Perazella M, Wilson F, Makhijani A, Shelton K, Parikh C, Moledina D. Adjudication of Kidney Biopsy Histopathology to Establish Consensus Diagnosis of Tubulointerstitial Nephritis. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.20241qexcb21. DOI: 10.1681/asn.20241qexcb21.Peer-Reviewed Original ResearchDevelopment of Urinalysis Screening Criteria for Electronic Health Alerts for Rhabdomyolysis
Yasmin F, Faulkner S, Aklilu A, Yamamoto Y, Moledina D, Wilson F. Development of Urinalysis Screening Criteria for Electronic Health Alerts for Rhabdomyolysis. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.2024bb8bw9yb. DOI: 10.1681/asn.2024bb8bw9yb.Peer-Reviewed Original ResearchEffect of Cessation of Renin-Angiotensin System Inhibitors on Clinical Outcomes in AKI
Makhijani A, Yasmin F, Moledina D, Yamamoto Y, Wilson F. Effect of Cessation of Renin-Angiotensin System Inhibitors on Clinical Outcomes in AKI. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.2024xcwjpmwx. DOI: 10.1681/asn.2024xcwjpmwx.Peer-Reviewed Original ResearchPlasma proteomics of acute tubular injury
Schmidt I, Surapaneni A, Zhao R, Upadhyay D, Yeo W, Schlosser P, Huynh C, Srivastava A, Palsson R, Kim T, Stillman I, Barwinska D, Barasch J, Eadon M, El-Achkar T, Henderson J, Moledina D, Rosas S, Claudel S, Verma A, Wen Y, Lindenmayer M, Huber T, Parikh S, Shapiro J, Rovin B, Stanaway I, Sathe N, Bhatraju P, Coresh J, Rhee E, Grams M, Waikar S. Plasma proteomics of acute tubular injury. Nature Communications 2024, 15: 7368. PMID: 39191768, PMCID: PMC11349760, DOI: 10.1038/s41467-024-51304-x.Peer-Reviewed Original ResearchConceptsAcute tubular injuryAcute kidney injuryPathway analysisOrganelle stress responsesSingle-cell RNA sequencingKidney Precision Medicine ProjectKidney diseaseSOMAscan proteomics platformProgression to kidney failureRNA sequencingBiological pathwaysGene expressionStress responseNon-invasive biomarkersProteomics platformMannose receptor 1Kidney injuryTubular injuryImmune regulationReceptor 1Macrophage mannose receptor 1Host responseBiomarker proteinsPrecision Medicine ProjectKidney failureThe development of lateral flow devices for urinary biomarkers to assess kidney health
D Souza S, Obeid W, Hernandez J, Hu D, Wen Y, Moledina D, Albert A, Gregg A, Wheeler A, Philbrook H, Parikh C. The development of lateral flow devices for urinary biomarkers to assess kidney health. Scientific Reports 2024, 14: 8516. PMID: 38609491, PMCID: PMC11014899, DOI: 10.1038/s41598-024-59104-5.Peer-Reviewed Original ResearchConceptsLateral flow deviceInterleukin-9Development of lateral flow devicesUrinary biomarkersDetection antibody concentrationSerum creatinine levelsKidney healthPaired antibodiesEnzyme Linked Immunosorbent AssayCreatinine levelsKidney injuryKidney functionOsteopontinImmunosorbent assayAntibody concentrationsMeasured biomarkersKidneyReal-time changesBiomarkers