2024
Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging
Schueder F, Rivera-Molina F, Su M, Marin Z, Kidd P, Rothman J, Toomre D, Bewersdorf J. Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging. Cell 2024, 187: 1769-1784.e18. PMID: 38552613, DOI: 10.1016/j.cell.2024.02.033.Peer-Reviewed Original ResearchConceptsInter-organelle contactsSuper-resolutionMultiplexed super-resolution microscopyIntricate spatial relationshipsGolgi stacksMammalian cellsCellular functionsSuper-resolution microscopyPrimary ciliaSuper-resolution fluorescence microscopyCellular complexityTransient adaptationFluorescence microscopyDNA-PAINTFluorogenic labelingMolecular targetsSpatial relationshipsImagesThroughput
2016
The IN/OUT assay: a new tool to study ciliogenesis
Kukic I, Rivera-Molina F, Toomre D. The IN/OUT assay: a new tool to study ciliogenesis. Cilia 2016, 5: 23. PMID: 27493724, PMCID: PMC4972980, DOI: 10.1186/s13630-016-0044-2.Peer-Reviewed Original ResearchCiliary pocketCiliary transmembrane proteinsPlasma membrane invaginationsDeep plasma membrane invaginationsPrimary cilia assemblyCilia assemblyMembrane invaginationsTransmembrane proteinPrimary ciliaCellular antennaCilia lengthMolecular characterizationMost ciliaFunctional studiesCiliogenesisCiliaNew toolRPE cellsCellsAssaysAntibody accessEHD1New assayPocketConventional assays
2015
The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135
Stoops EH, Hull M, Olesen C, Mistry K, Harder JL, Rivera-Molina F, Toomre D, Caplan MJ. The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135. Journal Of Cell Biology 2015, 211: 287-294. PMID: 26504168, PMCID: PMC4621837, DOI: 10.1083/jcb.201502045.Peer-Reviewed Original ResearchConceptsPrimary ciliaSurface proteinsTrans-Golgi networkPolarized epithelial cellsApical surface proteinsSNAP-tag systemBasolateral plasma membraneCell surface proteinsEpithelial cellsApical proteinsPericiliary regionGolgi networkPolarized traffickingCarrier vesiclesProtein deliveryPlasma membraneApical membraneProteinGp135Basolateral membraneCiliaMembraneHot spotsCellsTrafficking
2007
Par3 functions in the biogenesis of the primary cilium in polarized epithelial cells
Sfakianos J, Togawa A, Maday S, Hull M, Pypaert M, Cantley L, Toomre D, Mellman I. Par3 functions in the biogenesis of the primary cilium in polarized epithelial cells. Journal Of Cell Biology 2007, 179: 1133-1140. PMID: 18070914, PMCID: PMC2140027, DOI: 10.1083/jcb.200709111.Peer-Reviewed Original ResearchConceptsPrimary ciliaAtypical protein kinase CIntraflagellar transport particlesBasolateral membrane domainsMicrotubule-dependent transportProtein kinase CEpithelial cellsMembrane biogenesisPDZ proteinsMembrane domainsMembrane proteinsPar complexMicrotubule motorsCiliary membraneVectorial movementPar3 functionsKinesin-2Kinase CPar3Additional roleJunctional complexesCrumbs3Apical surfaceBiogenesisCilia