2018
S-Palmitoylation Sorts Membrane Cargo for Anterograde Transport in the Golgi
Ernst AM, Syed SA, Zaki O, Bottanelli F, Zheng H, Hacke M, Xi Z, Rivera-Molina F, Graham M, Rebane AA, Björkholm P, Baddeley D, Toomre D, Pincet F, Rothman JE. S-Palmitoylation Sorts Membrane Cargo for Anterograde Transport in the Golgi. Developmental Cell 2018, 47: 479-493.e7. PMID: 30458139, PMCID: PMC6251505, DOI: 10.1016/j.devcel.2018.10.024.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportCells, CulturedEndoplasmic ReticulumGolgi ApparatusHumansIntracellular MembranesLipoylationProtein TransportConceptsS-palmitoylationAnterograde cargoAnterograde signalMembrane cargoCargo selectionTransmembrane domainMembrane proteinsGolgi membranesGolgiSpecific signalsMembrane interfaceModel systemCargoProteinRate of transportAnterograde transportVesiclesCisternaeCurved regionsMembraneTransportRegionSignalsDomainFluorescence
2017
Land-locked mammalian Golgi reveals cargo transport between stable cisternae
Dunlop MH, Ernst AM, Schroeder LK, Toomre DK, Lavieu G, Rothman JE. Land-locked mammalian Golgi reveals cargo transport between stable cisternae. Nature Communications 2017, 8: 432. PMID: 28874656, PMCID: PMC5585379, DOI: 10.1038/s41467-017-00570-z.Peer-Reviewed Original Research
2011
Using light to see and control membrane traffic
Xu Y, Melia TJ, Toomre DK. Using light to see and control membrane traffic. Current Opinion In Chemical Biology 2011, 15: 822-830. PMID: 22079055, DOI: 10.1016/j.cbpa.2011.10.016.Peer-Reviewed Original Research
2008
Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
Goss JW, Toomre DK. Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis. Journal Of Cell Biology 2008, 181: 1047-1054. PMID: 18573914, PMCID: PMC2442215, DOI: 10.1083/jcb.200712137.Peer-Reviewed Original ResearchConceptsDaughter cellsTotal internal reflection fluorescence microscopy imagingCleavage furrowAdvanced live-cell imaging techniquesConfocal time-lapse imagingLive-cell imaging techniquesCell imaging techniquesReserve vesicle poolTime-lapse imagingMidbody abscissionMammalian cytokinesisFluorescence microscopy imagingFluorescent proteinPhotobleaching experimentsCytokinesisVesicle poolLysosomal compartmentIndividual vesiclesSingle vesiclesVesiclesGolgiFurrow regionMidbodyMembraneFurrow
2007
Internalization, Intracellular Trafficking, Biodistribution of Monoclonal Antibody 806: A Novel Anti-Epidermal Growth Factor Receptor Antibody
Perera RM, Zoncu R, Johns TG, Pypaert M, Lee FT, Mellman I, Old LJ, Toomre DK, Scott AM. Internalization, Intracellular Trafficking, Biodistribution of Monoclonal Antibody 806: A Novel Anti-Epidermal Growth Factor Receptor Antibody. Neoplasia 2007, 9: 1099-1110. PMID: 18084617, PMCID: PMC2134906, DOI: 10.1593/neo.07721.Peer-Reviewed Original ResearchConceptsEpidermal growth factor receptorMonoclonal antibody 806EGFR antibodyAnti-epidermal growth factor receptor (EGFR) antibodiesGrowth factor receptor antibodyHuman tumor xenograftsSynergistic antitumor activityNude mouse modelXenografted nude mouse modelSignificant therapeutic efficacyGrowth factor receptorPoor prognosisReceptor antibodiesEGFR therapeuticsEpithelial tumorsMouse modelTumor xenograftsNude miceSingle agentToxin conjugatesTherapeutic efficacyTherapeutic useTumor modelBiodistribution analysisTumor cells
2002
Selective Delivery of Secretory Cargo in Golgi‐Derived Carriers of Nonepithelial Cells
Rustom A, Bajohrs M, Kaether C, Keller P, Toomre D, Corbeil D, Gerdes H. Selective Delivery of Secretory Cargo in Golgi‐Derived Carriers of Nonepithelial Cells. Traffic 2002, 3: 279-288. PMID: 11929609, DOI: 10.1034/j.1600-0854.2002.030405.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsBiological TransportCell LineChlorocebus aethiopsChromograninsDogsDrug Delivery SystemsElectroporationEpithelial CellsGene DeletionGolgi ApparatusGreen Fluorescent ProteinsHumansImmunohistochemistryLuminescent ProteinsMicroscopy, ElectronMicroscopy, FluorescenceMutationPlasmidsRecombinant Fusion ProteinsRecombinant ProteinsTransfectionVero Cells