Cellular imaging using total internal reflection fluorescence microscopy: theory and instrumentation.
Toomre D. Cellular imaging using total internal reflection fluorescence microscopy: theory and instrumentation. Cold Spring Harbor Protocols 2012, 2012: 414-24. PMID: 22474668, DOI: 10.1101/pdb.top068650.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyReflection fluorescence microscopyRefractive indexHigh numerical aperture objective lensNumerical aperture objective lensThinner optical planeDifferent refractive indicesDynamic cellular processesFluorescence microscopyCell fluorescent microscopyTransient intermediate statesConventional epifluorescence microscopeEvanescent fieldMembrane traffickingDeep imagingObjective lensCellular processesCytoskeleton remodelingCell cortexOptical planeCell signalingAxial resolutionOptical principlesOrganelle levelMolecular manipulation