2021
Combined liver–cytokine humanization comes to the rescue of circulating human red blood cells
Song Y, Shan L, Gbyli R, Liu W, Strowig T, Patel A, Fu X, Wang X, Xu ML, Gao Y, Qin A, Bruscia EM, Tebaldi T, Biancon G, Mamillapalli P, Urbonas D, Eynon E, Gonzalez DG, Chen J, Krause DS, Alderman J, Halene S, Flavell RA. Combined liver–cytokine humanization comes to the rescue of circulating human red blood cells. Science 2021, 371: 1019-1025. PMID: 33674488, PMCID: PMC8292008, DOI: 10.1126/science.abe2485.Peer-Reviewed Original ResearchConceptsRed blood cellsBlood cellsHuman sickle cell diseaseSickle cell diseaseImmunodeficient murine modelKupffer cell densityBone marrow failureMISTRG miceIntrasplenic injectionSCD pathologyCell diseaseMurine modelComplement C3RBC survivalVivo modelHuman cytokinesPreclinical testingHematopoietic stem cellsHuman red blood cellsMarrow failureFumarylacetoacetate hydrolase geneHuman erythropoiesisHuman liverHuman hepatocytesMice
2016
ISL1 cardiovascular progenitor cells for cardiac repair after myocardial infarction
Bartulos O, Zhuang ZW, Huang Y, Mikush N, Suh C, Bregasi A, Wang L, Chang W, Krause DS, Young LH, Pober JS, Qyang Y. ISL1 cardiovascular progenitor cells for cardiac repair after myocardial infarction. JCI Insight 2016, 1: e80920. PMID: 27525311, PMCID: PMC4982472, DOI: 10.1172/jci.insight.80920.Peer-Reviewed Original ResearchMyocardial infarctionControl animalsCardiovascular progenitor cellsProgenitor cellsVentricular contractile functionCardiac repair strategiesNew blood vesselsInfarct areaLineage-tracing studiesContractile functionCardiac repairBlood vessel formationMyocardial regenerationEndothelial cellsHeart tissueBlood vesselsMurine heartInfarctionVessel formationInjuryMiceDelivery approachCardiomyocytesHeartCellsLeukaemia-associated Rho guanine nucleotide exchange factor (LARG) plays an agonist specific role in platelet function through RhoA activation
Zou S, Teixeira AM, Yin M, Xiang Y, Xavier-Ferrucio J, Zhang PX, Hwa J, Min W, Krause DS. Leukaemia-associated Rho guanine nucleotide exchange factor (LARG) plays an agonist specific role in platelet function through RhoA activation. Thrombosis And Haemostasis 2016, 116: 506-516. PMID: 27345948, PMCID: PMC5845781, DOI: 10.1160/th15-11-0848.Peer-Reviewed Original ResearchConceptsMegakaryocyte maturationPlatelet functionRhoA activationKO plateletsLeukemia-associated Rho guanineΑ-granule releasePlatelet signal transductionSmall molecule-mediated inhibitionExchange factorSignal transductionMyosin light chain phosphorylationRho guanineKO miceBleeding timeHuman megakaryocytesInternal bleedingPlatelet aggregationNormal haemostasisLight chain phosphorylationHuman plateletsVivo assaysPlateletsSpecific roleMiceChain phosphorylation
2015
Pharmacological modulation of the AKT/microRNA-199a-5p/CAV1 pathway ameliorates cystic fibrosis lung hyper-inflammation
Zhang PX, Cheng J, Zou S, D'Souza AD, Koff JL, Lu J, Lee PJ, Krause DS, Egan ME, Bruscia EM. Pharmacological modulation of the AKT/microRNA-199a-5p/CAV1 pathway ameliorates cystic fibrosis lung hyper-inflammation. Nature Communications 2015, 6: 6221. PMID: 25665524, PMCID: PMC4324503, DOI: 10.1038/ncomms7221.Peer-Reviewed Original ResearchConceptsCF macrophagesMiR-199aMicroRNA-199aHyper-inflammatory responseCFTR-deficient miceCystic fibrosis patientsCystic fibrosis lungLung destructionDisease morbidityPharmacological modulationCF miceCF lungFibrosis patientsInnate immunityLungMacrophagesCAV1 expressionDrug celecoxibReduced levelsTLR4CelecoxibMiceCav1PathwayMorbidity
2012
MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation
Smith EC, Thon JN, Devine MT, Lin S, Schulz VP, Guo Y, Massaro SA, Halene S, Gallagher P, Italiano JE, Krause DS. MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation. Blood 2012, 120: 2317-2329. PMID: 22806889, PMCID: PMC3447785, DOI: 10.1182/blood-2012-04-420828.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DiphosphateAnimalsBleeding TimeBlood PlateletsBone Marrow CellsCells, CulturedCrosses, GeneticCytoplasmCytoskeletonGene Expression ProfilingHematopoiesisMegakaryocytesMiceMice, Inbred C57BLMice, KnockoutOligonucleotide Array Sequence AnalysisPlatelet ActivationThrombocytopeniaTrans-ActivatorsTranscription FactorsConceptsMegakaryocyte maturationPlatelet formationSerum response factorSerum response factor expressionMembrane organizationGene expressionMKL1MKL2Response factorDKO miceKO backgroundMegakaryocyte compartmentMegakaryocytesCritical roleMegakaryocyte ploidyExpressionMaturationKnockout miceFactor expressionCrucial roleHomologuesGenesMiceProlonged bleeding timeRoleNonhematopoietic Cells are the Primary Source of Bone Marrow‐Derived Lung Epithelial Cells
Kassmer SH, Bruscia EM, Zhang P, Krause DS. Nonhematopoietic Cells are the Primary Source of Bone Marrow‐Derived Lung Epithelial Cells. Stem Cells 2012, 30: 491-499. PMID: 22162244, PMCID: PMC3725285, DOI: 10.1002/stem.1003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsBone Marrow CellsBone Marrow TransplantationCell SeparationEpithelial CellsGene ExpressionLuminescent ProteinsLungMiceMice, 129 StrainMice, Inbred C57BLMice, KnockoutMicroscopy, ConfocalPulmonary Surfactant-Associated Protein CRecombinant ProteinsSingle-Cell AnalysisConceptsLung epithelial cellsNonhematopoietic cellsBM cellsEpithelial cellsBone marrowLungs of miceType 2 pneumocytesNonhematopoietic stem cellsNonhematopoietic fractionAdult BMPrimitive stem cell populationNull miceProgenitor cellsMiceStem cell populationCell populationsMarrowStem cellsMultiple tissuesHematopoietic stemBMCellsPrevious studiesEngraftmentLungProxTom Lymphatic Vessel Reporter Mice Reveal Prox1 Expression in the Adrenal Medulla, Megakaryocytes, and Platelets
Truman LA, Bentley KL, Smith EC, Massaro SA, Gonzalez DG, Haberman AM, Hill M, Jones D, Min W, Krause DS, Ruddle NH. ProxTom Lymphatic Vessel Reporter Mice Reveal Prox1 Expression in the Adrenal Medulla, Megakaryocytes, and Platelets. American Journal Of Pathology 2012, 180: 1715-1725. PMID: 22310467, PMCID: PMC3349900, DOI: 10.1016/j.ajpath.2011.12.026.Peer-Reviewed Original ResearchMeSH KeywordsAdrenal MedullaAnimalsBlood PlateletsCells, CulturedCytoplasmEndothelial CellsGene Expression RegulationGenotypeGlycoproteinsHomeodomain ProteinsLuminescent ProteinsLymph NodesLymphatic VesselsMegakaryocytesMembrane Transport ProteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, FluorescenceTumor Cells, CulturedTumor Suppressor ProteinsConceptsLymph nodesLymphatic vesselsAdrenal medullaExpression of Prox1Tumor metastasisHigh endothelial venulesProx1 expressionTwo-photon laser scanning microscopyTransplant rejectionDentate gyrusEndothelial venulesAntigen presentationC57BL/6 backgroundTransgenic miceLipid metabolismMiceNeuroendocrine cellsAdult liverNovel siteMetastasisMedullaStudy of diseasesLiving mouseUnknown rolePotential utility
2008
Physiological variations of stem cell factor and stromal‐derived factor‐1 in murine models of liver injury and regeneration
Swenson ES, Kuwahara R, Krause DS, Theise ND. Physiological variations of stem cell factor and stromal‐derived factor‐1 in murine models of liver injury and regeneration. Liver International 2008, 28: 308-318. PMID: 18290773, PMCID: PMC2846401, DOI: 10.1111/j.1478-3231.2007.01659.x.Peer-Reviewed Original ResearchConceptsStromal-derived factor-1Oval cell proliferationLiver injuryLiver irradiationBile ductCell proliferationSDF-1 levelsArterial smooth muscleFactor 1Cell factorMarrow-derived progenitorsNormal mouse liverPlasma levelsBACKGROUND/Murine modelStem cell factorKupffer cellsSmooth muscleInjuryRegenerative responseOval cellsDihydrocollidineMouse liverMiceLiver progenitors
2007
Limitations of Green Fluorescent Protein as a Cell Lineage Marker
Swenson ES, Price JG, Brazelton T, Krause DS. Limitations of Green Fluorescent Protein as a Cell Lineage Marker. Stem Cells 2007, 25: 2593-2600. PMID: 17615263, DOI: 10.1634/stemcells.2007-0241.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood CellsCattleCell LineageChickensCytomegalovirusFemaleFluorescent Antibody TechniqueGene ExpressionGenes, ReporterGenes, SyntheticGreen Fluorescent ProteinsHumansImmunoenzyme TechniquesMaleMiceMice, Inbred C57BLOrgan SpecificityRegulatory Sequences, Nucleic AcidTissue DistributionTransgenesVisceraConceptsSmall intestineMouse strainsPeripheral blood cellsTransgenic mouse strainReporter mouse strainPrimary rabbit antibodiesDonor originHuman ubiquitin C promoterImmunohistochemical stainingSolid organsCell lineage markersCell lineagesUBC-GFP miceUbiquitin C promoterChicken beta-actinFlow cytometryBlood cellsEnhanced green fluorescent protein (EGFP) reporterMiceOne-tissueAdult liverTissue sectionsIntestineLineage markersRabbit antibodies
2006
Prevention of mesangial sclerosis by bone marrow transplantation
Guo J, Ardito TA, Kashgarian M, Krause DS. Prevention of mesangial sclerosis by bone marrow transplantation. Kidney International 2006, 70: 910-913. PMID: 16850025, DOI: 10.1038/sj.ki.5001698.Peer-Reviewed Original ResearchConceptsBone marrow transplantationMesangial sclerosisMarrow transplantationUrinary albumin lossSimilar therapeutic effectsOnset of diseaseWild-type BMIntrarenal administrationRenal functionRenal histologyRenal diseaseDisease onsetRenal pathologyBM cellsTherapeutic effectEngraftment levelsRenal cellsAlbumin lossKidney samplesMiceSclerosisTransplantationUntreated controlsDiseaseAdministrationSALL4, a novel oncogene, is constitutively expressed in human acute myeloid leukemia (AML) and induces AML in transgenic mice
Ma Y, Cui W, Yang J, Qu J, Di C, Amin HM, Lai R, Ritz J, Krause DS, Chai L. SALL4, a novel oncogene, is constitutively expressed in human acute myeloid leukemia (AML) and induces AML in transgenic mice. Blood 2006, 108: 2726-2735. PMID: 16763212, PMCID: PMC1895586, DOI: 10.1182/blood-2006-02-001594.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAnimalsApoptosisBase SequenceBeta CateninCloning, MolecularColony-Forming Units AssayDNA, ComplementaryDNA, NeoplasmDNA-Binding ProteinsGene ExpressionHematopoiesisHumansLeukemia, Myeloid, AcuteMiceMice, TransgenicMyelodysplastic SyndromesNeoplasm TransplantationOncogenesProtein IsoformsRNA, MessengerRNA, NeoplasmSignal TransductionTranscription FactorsWnt ProteinsConceptsAcute myeloid leukemiaMyeloid leukemiaMurine modelTransgenic miceHuman primary acute myeloid leukemiaMDS/acute myeloid leukemiaPrimary acute myeloid leukemiaHuman acute myeloid leukemiaLeukemia stem cellsAML transformationMyelodysplastic syndromePolymerase chain reactionWnt/beta-catenin pathwayZinc finger transcriptional factorNovel oncogeneBeta-catenin pathwayLeukemogenic potentialConstitutive expressionChain reactionPathway's roleLeukemiaSALL4MiceStem cellsMouse marrowAssessment of cystic fibrosis transmembrane conductance regulator (CFTR) activity in CFTR-null mice after bone marrow transplantation
Bruscia EM, Price JE, Cheng EC, Weiner S, Caputo C, Ferreira EC, Egan ME, Krause DS. Assessment of cystic fibrosis transmembrane conductance regulator (CFTR) activity in CFTR-null mice after bone marrow transplantation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 2965-2970. PMID: 16481627, PMCID: PMC1413802, DOI: 10.1073/pnas.0510758103.Peer-Reviewed Original ResearchConceptsCftr-/- miceEpithelial cellsNasal epitheliumBM-derived cellsBone marrow transplantationWild-type BMAirway epithelial cellsCystic fibrosis transmembrane conductance regulator (CFTR) activityCystic fibrosis miceRare epithelial cellsCftr-null miceMarrow transplantationBM transplantationFibrosis miceRespiratory tractCFTR activityGI tractBone marrowGastrointestinalChloride secretionCFTR-dependent chloride secretionIndividual miceTransplantationDifferent dosesMice
2005
Integration of engrafted Schwann cells into injured peripheral nerve: Axonal association and nodal formation on regenerated axons
Radtke C, Akiyama Y, Lankford KL, Vogt PM, Krause DS, Kocsis JD. Integration of engrafted Schwann cells into injured peripheral nerve: Axonal association and nodal formation on regenerated axons. Neuroscience Letters 2005, 387: 85-89. PMID: 16084645, PMCID: PMC2605373, DOI: 10.1016/j.neulet.2005.06.073.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxotomyCell Adhesion Molecules, NeuronalCell CompartmentationCytoplasmDisease Models, AnimalFemaleGreen Fluorescent ProteinsImmunohistochemistryMaleMiceMice, Inbred C57BLMicroscopy, Electron, TransmissionMyelin SheathNAV1.6 Voltage-Gated Sodium ChannelNerve RegenerationNerve Tissue ProteinsPeripheral Nerve InjuriesPeripheral NervesRanvier's NodesSchwann CellsSciatic NeuropathySodium ChannelsY ChromosomeConceptsWild-type miceSchwann cellsMyelin-forming cellsRegenerated axonsSodium channelsType miceRegenerated peripheral nerve fibersFemale wild-type miceDonor cellsMale donor cellsPeripheral nerve fibersSciatic nerve axonsImmuno-electron microscopic analysisCrush injuryCrush sitePeripheral nervesDonor originMale miceNerve fibersNerve axonsNodal formationAxonsNerveMiceAxonal associations