2021
Single cell epigenetic visualization assay
Kint S, Van Criekinge W, Vandekerckhove L, De Vos WH, Bomsztyk K, Krause DS, Denisenko O. Single cell epigenetic visualization assay. Nucleic Acids Research 2021, 49: e43-e43. PMID: 33511400, PMCID: PMC8096246, DOI: 10.1093/nar/gkab009.Peer-Reviewed Original ResearchMeSH Keywords5-MethylcytosineAcetylationCell LineDNA MethylationEarly Growth Response Protein 1Epigenesis, GeneticEpigenomicsFemaleGene Expression RegulationGene SilencingHistonesHIV-1HumansImage Processing, Computer-AssistedIn Situ Hybridization, FluorescenceProvirusesReal-Time Polymerase Chain ReactionRNA, Long NoncodingSingle-Cell AnalysisConceptsEpigenetic marksEpigenetic statusGene allelesFemale somatic cellsCurrent sequencing approachesGene of interestGene-specific oligonucleotidesQuantitative fluorescent readoutTranscription stateRNA FISHHuman cell linesSomatic cellsTranscription statusTarget genesSequencing approachH3K9ac levelsDifferent genesGenesIndividual cellsAntibody-conjugated alkaline phosphataseDNA oligosSingle cellsCell linesSame cellsFluorescent readout
2018
MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis
Rahman NT, Schulz VP, Wang L, Gallagher PG, Denisenko O, Gualdrini F, Esnault C, Krause DS. MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis. Blood Advances 2018, 2: 2691-2703. PMID: 30337297, PMCID: PMC6199649, DOI: 10.1182/bloodadvances.2018019448.Peer-Reviewed Original ResearchConceptsSerum response factorHEL cellsTarget genesBinding of SRFMegakaryocyte maturationActivity of SRFSRF target genesUpregulated target genesMyocardin family proteinsTernary complex factor familyTransformation-specific proteinsPrimary hematopoietic cellsHuman erythroleukemia cell lineErythroleukemia cell lineCArG sitesPrimary human CD34Genomic sitesGenomic regionsChromatin immunoprecipitationETS factorsTranscription factorsHuman megakaryopoiesisGenomic associationsMRTFAFactor family
2012
Induction of megakaryocyte differentiation drives nuclear accumulation and transcriptional function of MKL1 via actin polymerization and RhoA activation
Smith EC, Teixeira AM, Chen RC, Wang L, Gao Y, Hahn KL, Krause DS. Induction of megakaryocyte differentiation drives nuclear accumulation and transcriptional function of MKL1 via actin polymerization and RhoA activation. Blood 2012, 121: 1094-1101. PMID: 23243284, PMCID: PMC3575755, DOI: 10.1182/blood-2012-05-429993.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell DifferentiationCell Line, TumorCell NucleusDNA-Binding ProteinsEnzyme ActivationHumansMegakaryocyte Progenitor CellsMegakaryocytesMiceOncogene Proteins, FusionProtein MultimerizationRhoA GTP-Binding ProteinSerum Response FactorTetradecanoylphorbol AcetateThrombopoietinTrans-ActivatorsConceptsMegakaryocyte differentiationActin polymerizationSubcellular localizationSerum response factor (SRF) transcriptional activityRhoA activitySRF target genesComplex cellular responsesFactor transcriptional activityMuscle cell typesCell-type specificHuman erythroleukemia cellsPrimary megakaryocytesTranscriptional regulatorsActin organizationCellular functionsTranscriptional functionSRF activityNuclear localizationTarget genesMegakaryocytic differentiationTranscriptional activityNuclear accumulationErythroleukemia cellsMolecular mechanismsRhoA activation