A nuclear factor Y (NFY) site positively regulates the human CD34 stem cell gene.
Radomska H, Satterthwaite A, Taranenko N, Narravula S, Krause D, Tenen D. A nuclear factor Y (NFY) site positively regulates the human CD34 stem cell gene. Blood 1999, 94: 3772-80. PMID: 10572091, DOI: 10.1182/blood.v94.11.3772.423k19_3772_3780.Peer-Reviewed Original ResearchConceptsHuman CD34 geneUntranslated regionCD34 geneC-MybPromoter activityCell-cycle regulated expressionMurine CD34 geneC-Myb binding sitesElectrophoretic mobility shift assaysNuclear factor Y (NF-Y) sitesTranscription factor NFYCCAAT box motifStem cell genesMobility shift assaysOptimal promoter activityReporter gene activityAntibody supershift experimentsEarly hematopoietic cellsTransient transfection experimentsBox motifCombinatorial actionGene activityPromoter functionOligonucleotide competitionCis elements