2021
MRTFA: A critical protein in normal and malignant hematopoiesis and beyond
Reed F, Larsuel ST, Mayday MY, Scanlon V, Krause DS. MRTFA: A critical protein in normal and malignant hematopoiesis and beyond. Journal Of Biological Chemistry 2021, 296: 100543. PMID: 33722605, PMCID: PMC8079280, DOI: 10.1016/j.jbc.2021.100543.Peer-Reviewed Original ResearchConceptsMalignant hematopoiesisActin cytoskeleton dynamicsCritical cellular functionsResponse factorSerum response factorTranscription factor ACellular rolesImmediate early genesProtein partnersTranscriptional regulationCytoskeleton dynamicsCellular functionsTranscriptional targetsTranscription factorsCytoskeletal proteinsCritical proteinsMRTFAEarly genesCell typesChromosomal translocationsHematopoietic cellsCell growthFactor AHematopoiesisMuscle cells
2020
Current understanding of human megakaryocytic-erythroid progenitors and their fate determinants.
Kwon N, Thompson EN, Mayday MY, Scanlon V, Lu YC, Krause DS. Current understanding of human megakaryocytic-erythroid progenitors and their fate determinants. Current Opinion In Hematology 2020, 28: 28-35. PMID: 33186151, PMCID: PMC7737300, DOI: 10.1097/moh.0000000000000625.Peer-Reviewed Original ResearchConceptsMegakaryocyte-erythroid progenitorsFate decisionsCell fate decisionsMegakaryocytic-erythroid progenitorsGene expression patternsProgenitor cell biologyFate determinantsFate determinationCurrent understandingTranscription factorsCell biologyExpression patternsPluripotent progenitorsProgenitorsModel systemExtrinsic factorsBiologyDisease statesFateDevelopment leadEpigeneticsMegakaryocytesUnderstandingDiscoveryIsolation
2019
IFN-γ binds TPO to inhibit hematopoiesis
Krause DS. IFN-γ binds TPO to inhibit hematopoiesis. Blood 2019, 133: 2004-2005. PMID: 31072961, DOI: 10.1182/blood-2019-03-900977.Peer-Reviewed Original Research
2017
Hematopoietic defects in response to reduced Arhgap21
Xavier-Ferrucio J, Ricon L, Vieira K, Longhini AL, Lazarini M, Bigarella CL, Franchi G, Krause DS, Saad STO. Hematopoietic defects in response to reduced Arhgap21. Stem Cell Research 2017, 26: 17-27. PMID: 29212046, PMCID: PMC6084430, DOI: 10.1016/j.scr.2017.11.014.Peer-Reviewed Original ResearchConceptsErythroid commitmentProgenitor cellsSerial bone marrow transplantationHuman primary cellsProtein familyRho GTPasesHematopoietic progenitor cellsPhenotypic HSCsRho GTPaseHematopoietic defectsRhoC activityNegative regulatorARHGAP21Hematopoietic stemHematopoietic cellsMyeloid progenitorsProgenitor coloniesPrimary cellsBone marrow cellsCancer cellsFunctional aspectsHaploinsufficient miceMarrow cellsCellsGTPases
2015
Regulation of actin polymerization by tropomodulin-3 controls megakaryocyte actin organization and platelet biogenesis
Sui Z, Nowak RB, Sanada C, Halene S, Krause DS, Fowler VM. Regulation of actin polymerization by tropomodulin-3 controls megakaryocyte actin organization and platelet biogenesis. Blood 2015, 126: 520-530. PMID: 25964668, PMCID: PMC4513252, DOI: 10.1182/blood-2014-09-601484.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsApoptosisBlood PlateletsBlotting, WesternCell MembraneCell ProliferationCells, CulturedCytoplasmEmbryo, MammalianFemaleFluorescent Antibody TechniqueHematopoiesisHemorrhageImmunoprecipitationMegakaryocytesMiceMice, KnockoutMicroscopy, ConfocalMicroscopy, Electron, TransmissionMicroscopy, FluorescencePloidiesPolymerizationTropomodulinConceptsPlatelet biogenesisDemarcation membrane systemF-actinTropomodulin-3Organelle distributionProplatelet formationActin polymerizationF-actin cappingF-actin organizationF-actin cytoskeletonWild-type megakaryocytesActin cytoskeletonActin organizationMK differentiationTmod isoformsLarge proplateletsBiogenesisContractile bundlesActin filamentsDMS formationBinds tropomyosinBud sizeMK numberConfocal microscopyCytoskeleton
2012
Reducing Mitochondrial ROS Improves Disease-related Pathology in a Mouse Model of Ataxia-telangiectasia
D'Souza AD, Parish IA, Krause DS, Kaech SM, Shadel GS. Reducing Mitochondrial ROS Improves Disease-related Pathology in a Mouse Model of Ataxia-telangiectasia. Molecular Therapy 2012, 21: 42-48. PMID: 23011031, PMCID: PMC3538311, DOI: 10.1038/mt.2012.203.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtaxia TelangiectasiaAtaxia Telangiectasia Mutated ProteinsCatalaseCD8-Positive T-LymphocytesCell Cycle ProteinsDisease Models, AnimalDNA-Binding ProteinsHematopoiesisImmunologic MemoryLymphomaMiceMice, KnockoutMitochondriaProtein Serine-Threonine KinasesReactive Oxygen SpeciesThymus NeoplasmsTumor Suppressor ProteinsConceptsMitochondrial reactive oxygen speciesReactive oxygen speciesAtaxia telangiectasiaT cell developmental defectsDNA damage responseDisease ataxia telangiectasiaMitochondrial ROS productionOverexpression of catalaseATM kinaseRedox sensingDevelopmental defectsLatter phenotypePartial rescueBone marrow hematopoiesisCancer predispositionNull mouse modelMitochondrial dysfunctionMacrophage differentiationTORC1ROS productionCancer developmentOxygen speciesMouse modelTS pathologyMarrow hematopoiesisMKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation
Smith EC, Thon JN, Devine MT, Lin S, Schulz VP, Guo Y, Massaro SA, Halene S, Gallagher P, Italiano JE, Krause DS. MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation. Blood 2012, 120: 2317-2329. PMID: 22806889, PMCID: PMC3447785, DOI: 10.1182/blood-2012-04-420828.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DiphosphateAnimalsBleeding TimeBlood PlateletsBone Marrow CellsCells, CulturedCrosses, GeneticCytoplasmCytoskeletonGene Expression ProfilingHematopoiesisMegakaryocytesMiceMice, Inbred C57BLMice, KnockoutOligonucleotide Array Sequence AnalysisPlatelet ActivationThrombocytopeniaTrans-ActivatorsTranscription FactorsConceptsMegakaryocyte maturationPlatelet formationSerum response factorSerum response factor expressionMembrane organizationGene expressionMKL1MKL2Response factorDKO miceKO backgroundMegakaryocyte compartmentMegakaryocytesCritical roleMegakaryocyte ploidyExpressionMaturationKnockout miceFactor expressionCrucial roleHomologuesGenesMiceProlonged bleeding timeRole
2006
Prevention of mesangial sclerosis by bone marrow transplantation
Guo J, Ardito TA, Kashgarian M, Krause DS. Prevention of mesangial sclerosis by bone marrow transplantation. Kidney International 2006, 70: 910-913. PMID: 16850025, DOI: 10.1038/sj.ki.5001698.Peer-Reviewed Original ResearchConceptsBone marrow transplantationMesangial sclerosisMarrow transplantationUrinary albumin lossSimilar therapeutic effectsOnset of diseaseWild-type BMIntrarenal administrationRenal functionRenal histologyRenal diseaseDisease onsetRenal pathologyBM cellsTherapeutic effectEngraftment levelsRenal cellsAlbumin lossKidney samplesMiceSclerosisTransplantationUntreated controlsDiseaseAdministrationSALL4, a novel oncogene, is constitutively expressed in human acute myeloid leukemia (AML) and induces AML in transgenic mice
Ma Y, Cui W, Yang J, Qu J, Di C, Amin HM, Lai R, Ritz J, Krause DS, Chai L. SALL4, a novel oncogene, is constitutively expressed in human acute myeloid leukemia (AML) and induces AML in transgenic mice. Blood 2006, 108: 2726-2735. PMID: 16763212, PMCID: PMC1895586, DOI: 10.1182/blood-2006-02-001594.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAnimalsApoptosisBase SequenceBeta CateninCloning, MolecularColony-Forming Units AssayDNA, ComplementaryDNA, NeoplasmDNA-Binding ProteinsGene ExpressionHematopoiesisHumansLeukemia, Myeloid, AcuteMiceMice, TransgenicMyelodysplastic SyndromesNeoplasm TransplantationOncogenesProtein IsoformsRNA, MessengerRNA, NeoplasmSignal TransductionTranscription FactorsWnt ProteinsConceptsAcute myeloid leukemiaMyeloid leukemiaMurine modelTransgenic miceHuman primary acute myeloid leukemiaMDS/acute myeloid leukemiaPrimary acute myeloid leukemiaHuman acute myeloid leukemiaLeukemia stem cellsAML transformationMyelodysplastic syndromePolymerase chain reactionWnt/beta-catenin pathwayZinc finger transcriptional factorNovel oncogeneBeta-catenin pathwayLeukemogenic potentialConstitutive expressionChain reactionPathway's roleLeukemiaSALL4MiceStem cellsMouse marrow
2001
Xenotransplantation of immunodeficient mice with mobilized human blood CD34+ cells provides an in vivo model for human megakaryocytopoiesis and platelet production
Perez L, Rinder H, Wang C, Tracey J, Maun N, Krause D. Xenotransplantation of immunodeficient mice with mobilized human blood CD34+ cells provides an in vivo model for human megakaryocytopoiesis and platelet production. Blood 2001, 97: 1635-1643. PMID: 11238102, DOI: 10.1182/blood.v97.6.1635.Peer-Reviewed Original ResearchConceptsPeripheral blood stem cellsHuman peripheral blood stem cellsPlatelet productionVivo modelStudy of megakaryocytopoiesisCFU-MKHuman megakaryocytopoiesisImmunodeficient miceBone marrowHuman plateletsExogenous cytokinesNOD/SCID miceHuman hematopoiesisBlood stem cellsHuman cell engraftmentPlatelet developmentNonobese diabetic/Lymphoid lineageStem cellsHuman blood CD34MegakaryocytopoiesisPeripheral bloodCytokine stimulationMurine recipientsThrombin stimulationHematopoietic Stem Cells Can Be CD34+ or CD34-
Donnelly D, Krause D. Hematopoietic Stem Cells Can Be CD34+ or CD34-. Leukemia & Lymphoma 2001, 40: 221-234. PMID: 11426544, DOI: 10.3109/10428190109057921.Peer-Reviewed Original Research
1997
Gotta find GATA a friend
Krause D, Perkins A. Gotta find GATA a friend. Nature Medicine 1997, 3: 960-961. PMID: 9288719, DOI: 10.1038/nm0997-960.Commentaries, Editorials and LettersRegulation of CD34 expression in differentiating M1 cells.
Krause DS, Kapadia SU, Raj NB, May WS. Regulation of CD34 expression in differentiating M1 cells. Experimental Hematology 1997, 25: 1051-61. PMID: 9293902.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsAntigens, CD34Base SequenceBinding SitesCell DifferentiationCells, CulturedDNA-Binding ProteinsDown-RegulationGene Expression RegulationGene Expression Regulation, DevelopmentalGene Expression Regulation, NeoplasticHematopoiesisLeukemia, MyeloidMiceMolecular Sequence DataNuclear ProteinsRNA, MessengerTranscription, GeneticConceptsTranscription initiation siteUntranslated regionPromoter activityHematopoietic stemCell type-specific expressionSecondary structureTATA-less promoterPromoter-luciferase reporter constructsFull promoter activityUpstream genomic DNAProgenitor cellsTranslation start siteMature blood cellsType-specific expressionOptimal promoter activityExtensive secondary structureP1 nuclease digestionCell-specific factorsTranscriptional initiationGene regulationTranscription factorsConsensus sitesStart siteRegulatory elementsTATA elementMultilineage gene expression precedes commitment in the hemopoietic system.
Hu M, Krause D, Greaves M, Sharkis S, Dexter M, Heyworth C, Enver T. Multilineage gene expression precedes commitment in the hemopoietic system. Genes & Development 1997, 11: 774-785. PMID: 9087431, DOI: 10.1101/gad.11.6.774.Peer-Reviewed Original ResearchConceptsGene expression programsMultilineage gene expressionLineage specificationExpression programsGene activityLocus activationMultipotential stateGene expressionCytokine receptorsHemopoietic stemGranulocytic lineageProgenitor cellsSingle-cell RT-PCRSame cellsHemopoietic systemRT-PCRExclusive commitmentCell RT-PCRCellsLineagesCoexpressionDifferentiationExpressionStemActivation