2008
Correction of a splice-site mutation in the beta-globin gene stimulated by triplex-forming peptide nucleic acids
Chin JY, Kuan JY, Lonkar PS, Krause DS, Seidman MM, Peterson KR, Nielsen PE, Kole R, Glazer PM. Correction of a splice-site mutation in the beta-globin gene stimulated by triplex-forming peptide nucleic acids. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 13514-13519. PMID: 18757759, PMCID: PMC2533221, DOI: 10.1073/pnas.0711793105.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell LineGene Expression RegulationGenomeGlobinsHumansMolecular Sequence DataMutationPeptide Nucleic AcidsRNA Splice SitesConceptsBeta-globin geneEndogenous beta-globin locusSplice site mutationHuman cellsPrimary hematopoietic progenitor cellsBeta-globin locusAltered helical structureHuman beta-globin geneSingle base pair changeSingle base-pair modificationTriplex-forming peptide nucleic acidsDonor DNA moleculesBase pair changesCell cycle stageDisease-related genesDonor DNA fragmentsNucleic acidsProper splicingSite-specific bindingSite-specific modificationMammalian cellsHematopoietic progenitor cellsDNA repairSecond intronGene locus
2007
Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation
Ma X, Renda MJ, Wang L, Cheng EC, Niu C, Morris SW, S. AS, Krause DS. Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation. Molecular And Cellular Biology 2007, 27: 3056-3064. PMID: 17283045, PMCID: PMC1899951, DOI: 10.1128/mcb.01339-06.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBasic Helix-Loop-Helix Transcription FactorsCell NucleusCHO CellsCricetinaeCricetulusDNA-Binding ProteinsDrosophila ProteinsGene Expression ProfilingHomeodomain ProteinsImmunoglobulin J Recombination Signal Sequence-Binding ProteinMiceMolecular Sequence DataMyeloid CellsMyelopoiesisNuclear ProteinsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryProtein TransportReceptors, NotchRNA-Binding ProteinsRNA, MessengerRNA, Small InterferingTranscription Factor HES-1Transcription, GeneticTranscriptional ActivationConceptsN-terminusPromoter activityMyeloid differentiationCell linesCell type-specific mannerMyeloid precursor cell linePrimary murine cellsType-specific mannerDominant negative effectStimulation of NotchHematopoietic cell linesHuman erythroleukemia cellsPrecursor cell lineMurine cell linesHematopoietic stem cellsTranscriptional activationHes1 transcriptionRNA interferenceErythroleukemia cellsFusion proteinHes1 promoter activityMurine cellsFusion partnerHematopoietic cellsRBM15
1999
A nuclear factor Y (NFY) site positively regulates the human CD34 stem cell gene.
Radomska H, Satterthwaite A, Taranenko N, Narravula S, Krause D, Tenen D. A nuclear factor Y (NFY) site positively regulates the human CD34 stem cell gene. Blood 1999, 94: 3772-80. PMID: 10572091, DOI: 10.1182/blood.v94.11.3772.423k19_3772_3780.Peer-Reviewed Original ResearchConceptsHuman CD34 geneUntranslated regionCD34 geneC-MybPromoter activityCell-cycle regulated expressionMurine CD34 geneC-Myb binding sitesElectrophoretic mobility shift assaysNuclear factor Y (NF-Y) sitesTranscription factor NFYCCAAT box motifStem cell genesMobility shift assaysOptimal promoter activityReporter gene activityAntibody supershift experimentsEarly hematopoietic cellsTransient transfection experimentsBox motifCombinatorial actionGene activityPromoter functionOligonucleotide competitionCis elements
1997
Regulation of CD34 expression in differentiating M1 cells.
Krause DS, Kapadia SU, Raj NB, May WS. Regulation of CD34 expression in differentiating M1 cells. Experimental Hematology 1997, 25: 1051-61. PMID: 9293902.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsAntigens, CD34Base SequenceBinding SitesCell DifferentiationCells, CulturedDNA-Binding ProteinsDown-RegulationGene Expression RegulationGene Expression Regulation, DevelopmentalGene Expression Regulation, NeoplasticHematopoiesisLeukemia, MyeloidMiceMolecular Sequence DataNuclear ProteinsRNA, MessengerTranscription, GeneticConceptsTranscription initiation siteUntranslated regionPromoter activityHematopoietic stemCell type-specific expressionSecondary structureTATA-less promoterPromoter-luciferase reporter constructsFull promoter activityUpstream genomic DNAProgenitor cellsTranslation start siteMature blood cellsType-specific expressionOptimal promoter activityExtensive secondary structureP1 nuclease digestionCell-specific factorsTranscriptional initiationGene regulationTranscription factorsConsensus sitesStart siteRegulatory elementsTATA element
1996
CD34: Structure, Biology, and Clinical Utility
Krause D, Fackler M, Civin C, May W. CD34: Structure, Biology, and Clinical Utility. Blood 1996, 87: 1-13. PMID: 8547630, DOI: 10.1182/blood.v87.1.1.1.Peer-Reviewed Original ResearchAcute DiseaseAmino Acid SequenceAnimalsAntibodies, MonoclonalAntigens, CD34Antigens, NeoplasmBiomarkersCell AdhesionEndothelium, VascularFibroblastsGene Expression RegulationGenesHematopoietic Stem CellsHumansLeukemia, MyeloidMiceMolecular Sequence DataOrgan SpecificityProtein ConformationRNA Splicing
1995
Full-Length But Not Truncated CD34 Inhibits Hematopoietic Ceil Differentiation of Ml Cells
Fackler M, Krause D, Smith O, Civin C, May W. Full-Length But Not Truncated CD34 Inhibits Hematopoietic Ceil Differentiation of Ml Cells. Blood 1995, 85: 3040-3047. PMID: 7538813, DOI: 10.1182/blood.v85.11.3040.bloodjournal85113040.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAnimalsAntigens, CDAntigens, CD34Base SequenceCell DifferentiationGene Expression Regulation, LeukemicGrowth InhibitorsHematopoietic Stem CellsInterleukin-6Leukemia Inhibitory FactorLeukemia, MyeloidLymphokinesMacrophagesMiceMolecular Sequence DataPeptide FragmentsPhagocytosisRecombinant Fusion ProteinsTumor Cells, CulturedConceptsFull-length CD34Developmental stage-specific mannerM1 myeloid leukemia cellsTerminal myeloid differentiationMurine hematopoietic stemStage-specific mannerProgenitor cellsHematopoietic cell differentiationCD34 proteinNegative regulatory roleCytoplasmic tail domainMurine M1 myeloid leukemia cellsStem/progenitor cellsArrest phenotypeFunction of CD34Tail domainTerminal differentiationCell differentiationRegulatory roleMyeloid differentiationHematopoietic stemDomain regionLeukemia inhibitory factorHematopoietic cellsMyeloid leukemia cells
1994
Characterization of Murine CD34, a Marker for Hematopoietic Progenitor and Stem Cells
Krause D, Ito T, Fackler M, Smith O, Collector M, Sharkis S, May W. Characterization of Murine CD34, a Marker for Hematopoietic Progenitor and Stem Cells. Blood 1994, 84: 691-701. PMID: 7519070, DOI: 10.1182/blood.v84.3.691.691.Peer-Reviewed Original ResearchConceptsFull-length CD34Colony-forming unit-spleenM1 leukemia cellsMurine CD34Stem cellsHematopoietic progenitorsMurine hematopoietic stem cellsProgenitor cellsBone marrow cellsEmbryonic stem cellsHuman hematopoietic stemPotential functional differencesLong-term repopulating abilityCell linesFunctional hematopoietic progenitorsFull-length formCHO-K1 cell lineDifferent cell typesMurine cell linesPattern of expressionPA6 stromal cellsHematopoietic stem cellsLeukemia cellsHematopoietic progenitor cellsApparent molecular weight