2024
Cell surface RNAs control neutrophil recruitment
Zhang N, Tang W, Torres L, Wang X, Ajaj Y, Zhu L, Luan Y, Zhou H, Wang Y, Zhang D, Kurbatov V, Khan S, Kumar P, Hidalgo A, Wu D, Lu J. Cell surface RNAs control neutrophil recruitment. Cell 2024, 187: 846-860.e17. PMID: 38262409, PMCID: PMC10922858, DOI: 10.1016/j.cell.2023.12.033.Peer-Reviewed Original ResearchConceptsCell surfaceMammalian homologOuter cell surfaceRNA transportGlycan modificationsMammalian cellsSID-1Cellular functionsRecruitment to inflammatory sitesGlycoRNARNAMurine neutrophilsFunctional significanceNeutrophil recruitmentNeutrophil recruitment to inflammatory sitesBiological importanceCellsNeutrophil adhesionReduced neutrophil adhesionHomologyGlycansGenesInflammatory sitesRecruitmentEndothelial cells
2023
Cell Surface RNAs Control Neutrophil Function
Zhang N, Tang W, Torres L, Zhu L, Wang X, Ajaj Y, Wang Y, Zhang D, Kurbatov V, Zhou H, Luan Y, Kumar P, Hidalgo A, Wu D, Lu J. Cell Surface RNAs Control Neutrophil Function. Blood 2023, 142: 674. DOI: 10.1182/blood-2023-187570.Peer-Reviewed Original ResearchExtracellular RNaseCell surfaceTotal RNABona fide ligandsEndothelial cellsOuter cell surfaceTransendothelial migrationMammalian cellsSuch RNAsGlycan modificationsCellular RNAGlycoRNARNase digestionLive cellsRNAHematopoietic cellsRNase treatmentSimilar defectsIntegrin levelsConfocal microscopyRNaseGlycan fractionImportant functionsHomologuesRecombinant E-selectin
2012
P‐Rex1 is critical for vascular hyper‐permeability and edema in the lungs
Naikawadi R, Cheng N, Vogel S, Wu D, Malik A, Ye R. P‐Rex1 is critical for vascular hyper‐permeability and edema in the lungs. The FASEB Journal 2012, 26: 842.10-842.10. DOI: 10.1096/fasebj.26.1_supplement.842.10.Peer-Reviewed Original ResearchHuman lung microvascular endothelial cellsCultured human lung microvascular endothelial cellsEndothelial cellsLung vascular injuryLung microvascular endothelial cellsPotential therapeutic targetNeutrophil transendothelial migrationEndothelial barrier disruptionMicrovascular endothelial cellsICAM-1 inductionP-Rex1G protein-coupled receptorsReactive oxygen species productionNeutrophil infiltrationProtein-coupled receptorsBarrier disruptionIntercellular gap formationVascular injurySmall interference RNAInflammatory tissueOxygen species productionVascular permeabilityPI3K dependent mannerKnockout miceTherapeutic target
2011
A non‐GPCR dependent role for P‐Rex1 in endothelial cells
Naikawadi R, Cheng N, Wu D, Ye R. A non‐GPCR dependent role for P‐Rex1 in endothelial cells. The FASEB Journal 2011, 25: 631.9-631.9. DOI: 10.1096/fasebj.25.1_supplement.631.9.Peer-Reviewed Original ResearchHuman lung microvascular endothelial cellsTransendothelial electrical resistanceEndothelial cellsCultured human lung microvascular endothelial cellsLung vascular injuryVascular endothelial functionLung microvascular endothelial cellsVital organ functionLoss of TNFNeutrophil transendothelial migrationMicrovascular endothelial cellsVascular endothelial integrityTNF-α signalingP-Rex1Reactive oxygen species productionNeutrophil infiltrationEndothelial functionIntercellular gap formationVascular injurySmall interference RNAInflammatory tissueOxygen species productionVascular permeabilityTherapeutic targetOrgan function
2010
P‐Rex1 regulates lung microvascular permeability
Naikawadi R, Cheng N, Wu D, Ye R. P‐Rex1 regulates lung microvascular permeability. The FASEB Journal 2010, 24: lb554-lb554. DOI: 10.1096/fasebj.24.1_supplement.lb554.Peer-Reviewed Original ResearchAcute lung injuryLung microvascular permeabilityPolymorphonuclear leukocytesLung injuryEndothelial cellsP-Rex1 expressionMicrovascular permeabilityLung microvascular endothelial cellsGram-negative bacterial infectionsTNF-alpha contributesWild-type miceMicrovascular endothelial cellsEndothelial barrier permeabilityP-Rex1Endothelial cell signalingBacterial clearanceTNF-alphaReactive oxygen species generationBarrier permeabilityType miceMajor cytokineKnockout miceBacterial infectionsSepticemic infectionUncontrolled activation
2006
Corrigendum to “Role of guanine nucleotide exchange factor P-Rex-2b in sphingosine 1-phosphate-induced Rac1 activation and cell migration in endothelial cells” [Prostag. Oth. Lipid. M. 76 (2005) 95–104]
Li Z, Paik J, Wang Z, Hla T, Wu D. Corrigendum to “Role of guanine nucleotide exchange factor P-Rex-2b in sphingosine 1-phosphate-induced Rac1 activation and cell migration in endothelial cells” [Prostag. Oth. Lipid. M. 76 (2005) 95–104]. Prostaglandins And Other Lipid Mediators 2006, 79: 195. DOI: 10.1016/j.prostaglandins.2005.10.003.Peer-Reviewed Original Research