2001
Gα16 Couples Chemoattractant Receptors to NF-κB Activation
Yang M, Sang H, Rahman A, Wu D, Malik A, Ye R. Gα16 Couples Chemoattractant Receptors to NF-κB Activation. The Journal Of Immunology 2001, 166: 6885-6892. PMID: 11359849, DOI: 10.4049/jimmunol.166.11.6885.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAmino Acid SubstitutionCell NucleusEnzyme ActivationGenes, ReporterGlutamineGTP-Binding Protein alpha Subunits, Gq-G11HeLa CellsHeterotrimeric GTP-Binding ProteinsHumansIsoenzymesLeucineLuciferasesNF-kappa BPhospholipase C betaReceptors, ChemokineReceptors, Formyl PeptideReceptors, ImmunologicReceptors, PeptideSignal TransductionTrans-ActivatorsTransfectionType C PhospholipasesConceptsNF-kappaB activationFormyl peptide receptorChemoattractant receptorsPeptide receptorCXC chemokine receptor 2NF-kappaB.Chemokine receptor 2Regulatory protein alpha subunitNF-κB activationLuciferase activityProtein kinase CKappaB-luciferase activityPhosphorylation of IkappaBalphaPhospholipase C-beta activityC3a receptorReceptor 2Seven-membrane-spanning receptorsAgonist stimulationConventional PKC isoformsPharmacological inhibitorsLuciferase reporterReceptorsHemopoietic cellsProtein alpha subunitsCell model system
1998
Guanine nucleotide exchange factor GEF115 specifically mediates activation of Rho and serum response factor by the G protein α subunit Gα13
Mao J, Yuan H, Xie W, Wu D. Guanine nucleotide exchange factor GEF115 specifically mediates activation of Rho and serum response factor by the G protein α subunit Gα13. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 12973-12976. PMID: 9789025, PMCID: PMC23675, DOI: 10.1073/pnas.95.22.12973.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAnimalsCOS CellsDNA-Binding ProteinsGene Expression RegulationGTP-Binding ProteinsGTPase-Activating ProteinsGuanine Nucleotide Exchange FactorsKineticsLuciferasesMiceMolecular Sequence DataNuclear ProteinsProteinsRecombinant Fusion ProteinsRhoA GTP-Binding ProteinSequence AlignmentSequence Homology, Amino AcidSerum Response FactorSignal TransductionTranscription FactorsTranscription, GeneticTransfectionConceptsSerum response factorActivation of RhoSRF activationHeterotrimeric G protein alpha subunitsG protein signaling (RGS) domainG protein alpha subunitsTerminal partRho-specific guanineProtein alpha subunitsResponse factorDomain deletion mutantSignal transduction pathwaysNIH 3T3 cellsDbl homologyRGS activitySequence motifsTransduction pathwaysGalpha13Signaling domainsGalpha12Alpha subunitMutantsSynergistic activationGalphaqTransfection system
1992
Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells.
Wu D, Lee C, Rhee S, Simon M. Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells. Journal Of Biological Chemistry 1992, 267: 1811-1817. PMID: 1309799, DOI: 10.1016/s0021-9258(18)46018-8.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesCell LineCell MembraneEnzyme ActivationGTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)InositolInositol PhosphatesIsoenzymesKineticsMacromolecular SubstancesMolecular Sequence DataMutagenesis, Site-DirectedPeptidesPhosphatidylinositol Diacylglycerol-LyasePhosphoinositide Phospholipase CPhosphoric Diester HydrolasesTransfectionType C PhospholipasesConceptsG alpha 11G alpha qCos-7 cellsG alpha zAlpha qPI-PLCAlpha 11Alpha subunitAlpha zG protein alpha subunitsProtein alpha subunitsAlpha-subunit cDNAHigh steady-state levelsPhospholipase C beta 1Introduction of mutationsCell-free systemHigh-efficiency transient transfectionG protein activationC beta 1Glutamine 205Steady-state levelsBeta 1Transient transfectionProtein activationLeucine mutant
1991
Antibodies to the alpha q subfamily of guanine nucleotide-binding regulatory protein alpha subunits attenuate activation of phosphatidylinositol 4,5-bisphosphate hydrolysis by hormones.
Gutowski S, Smrcka A, Nowak L, Wu D, Simon M, Sternweis P. Antibodies to the alpha q subfamily of guanine nucleotide-binding regulatory protein alpha subunits attenuate activation of phosphatidylinositol 4,5-bisphosphate hydrolysis by hormones. Journal Of Biological Chemistry 1991, 266: 20519-20524. PMID: 1657928, DOI: 10.1016/s0021-9258(18)54955-3.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsBlotting, WesternBradykininCell LineElectrophoresis, Polyacrylamide GelGTP-Binding ProteinsHormonesHydrolysisImmune SeraPhosphatidylinositol 4,5-DiphosphatePhosphatidylinositol Diacylglycerol-LyasePhosphatidylinositol PhosphatesPhosphoric Diester HydrolasesPrecipitin TestsRatsConceptsAlpha qHormonal regulationNG108-15 cellsBisphosphate hydrolysisPhospholipase CRegulatory protein alphaPhospholipase C activityAlpha 11Attenuate activationG protein alpha subunitsAffinity-purified antibodiesProtein alphaProtein alpha subunitsAntibodiesGuanosine 5'Rat liverGq familyCommon carboxyl terminusC activityPhospholipase C.Alpha subunitDetergent extractsAntiserumActivationPeptides