1997
Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*
Xie W, Jiang H, Wu Y, Wu D. Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*. Journal Of Biological Chemistry 1997, 272: 24948-24951. PMID: 9312098, DOI: 10.1074/jbc.272.40.24948.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDArginineBinding SitesCOS CellsGTP-Binding ProteinsInterleukin-8KineticsLysineMolecular Sequence DataMutagenesis, Site-DirectedPhosphatidylinositol Diacylglycerol-LyaseProtein Structure, SecondaryReceptors, InterleukinReceptors, Interleukin-8ARecombinant Fusion ProteinsTransfectionType C Phospholipases
1996
Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma.
Kuang Y, Wu Y, Smrcka A, Jiang H, Wu D. Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 2964-2968. PMID: 8610151, PMCID: PMC39743, DOI: 10.1073/pnas.93.7.2964.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDBlotting, WesternCell LineChlorocebus aethiopsDNA, ComplementaryGlutamic AcidGTP-Binding ProteinsHumansIsoenzymesKidneyMacromolecular SubstancesMammalsMutagenesis, Site-DirectedPoint MutationPolymerase Chain ReactionReceptor, Anaphylatoxin C5aReceptors, ComplementRecombinant Fusion ProteinsRecombinant ProteinsTransfectionType C PhospholipasesConceptsCOS-7 cellsGlutathione S-transferaseGbeta gammaPLC-beta2Fusion proteinBeta gamma subunitsLigand-induced activationAlpha1B-adrenergic receptorMammalian expression plasmidX boxG proteinsY boxExpression plasmidAmino acidsS-transferaseProteinSequenceResiduesCellsActivationBeta2GalphaGbetaC5a receptorCDNA
1995
Different α1-Adrenergic Receptor Sequences Required for Activating Different Gα Subunits of Gq Class of G Proteins (∗)
Wu D, Jiang H, Simon M. Different α1-Adrenergic Receptor Sequences Required for Activating Different Gα Subunits of Gq Class of G Proteins (∗). Journal Of Biological Chemistry 1995, 270: 9828-9832. PMID: 7730363, DOI: 10.1074/jbc.270.17.9828.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell LineGTP-Binding ProteinsMolecular Sequence DataMutagenesis, Site-DirectedReceptors, Adrenergic, alpha-1ConceptsG alpha 16G alpha 14Gq classAlpha 16Receptor sequencesDifferent G alpha subunitsAlpha subunitG proteinsG alpha q/11Different Gα subunitsG alpha subunitsAmino acid stretchAlpha q/11Alpha 14N-terminal segmentThird inner loopGα subunitsBBXXB motifRegulatory proteinsC-terminusN-terminusSpecific mutationsSubunitsProteinSpecific interactions
1992
Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells.
Wu D, Lee C, Rhee S, Simon M. Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells. Journal Of Biological Chemistry 1992, 267: 1811-1817. PMID: 1309799, DOI: 10.1016/s0021-9258(18)46018-8.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesCell LineCell MembraneEnzyme ActivationGTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)InositolInositol PhosphatesIsoenzymesKineticsMacromolecular SubstancesMolecular Sequence DataMutagenesis, Site-DirectedPeptidesPhosphatidylinositol Diacylglycerol-LyasePhosphoinositide Phospholipase CPhosphoric Diester HydrolasesTransfectionType C PhospholipasesConceptsG alpha 11G alpha qCos-7 cellsG alpha zAlpha qPI-PLCAlpha 11Alpha subunitAlpha zG protein alpha subunitsProtein alpha subunitsAlpha-subunit cDNAHigh steady-state levelsPhospholipase C beta 1Introduction of mutationsCell-free systemHigh-efficiency transient transfectionG protein activationC beta 1Glutamine 205Steady-state levelsBeta 1Transient transfectionProtein activationLeucine mutant