1993
Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins.
Wu D, Jiang H, Katz A, Simon M. Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins. Journal Of Biological Chemistry 1993, 268: 3704-3709. PMID: 8381437, DOI: 10.1016/s0021-9258(18)53750-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarbacholCell LineCloning, MolecularEnzyme ActivationGTP-Binding ProteinsInositol PhosphatesKineticsMolecular Sequence DataMutagenesisNorepinephrinePhosphatidylinositol Diacylglycerol-LyasePhosphoric Diester HydrolasesPlasmidsReceptors, MuscarinicRecombinant ProteinsRestriction MappingSequence DeletionTransfectionConceptsG alpha q.PLC-beta 1COS-7 cellsG alpha qAlpha q.Alpha subunitG protein alpha subunitsAlpha qMuscarinic acetylcholine receptor type 1G alpha subunitsPhosphoinositide-specific phospholipase CIntrinsic enzymatic activitySpecific deletionAmino acid sequenceLigand-induced releasePhospholipase C beta 1C beta 1CDNA clonesAcid sequenceC-terminusG proteinsTerminal endDependent activationPhospholipase CAlpha 1C-adrenergic receptor
1992
Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C
Katz A, Wu D, Simon M. Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C. Nature 1992, 360: 686-689. PMID: 1465134, DOI: 10.1038/360686a0.Peer-Reviewed Original ResearchConceptsΑ-subunitG proteinsExchange of GDPVitro reconstitution experimentsReceptor-mediated releaseDifferent G proteinsFree GβγPhospholipase C activationGβγ complexG proteins resultsGα subunitsHL-60 cellsGβγ subunitsTransfection assaysPertussis toxinΓ subunitProtein resultsGβγReconstitution experimentsDifferent isoformsSpecific receptorsΒ2 isoformPhospholipase CΒ1 isoformsSubunitsActivation of phospholipase C by alpha 1-adrenergic receptors is mediated by the alpha subunits of Gq family.
Wu D, Katz A, Lee C, Simon M. Activation of phospholipase C by alpha 1-adrenergic receptors is mediated by the alpha subunits of Gq family. Journal Of Biological Chemistry 1992, 267: 25798-25802. PMID: 1334487, DOI: 10.1016/s0021-9258(18)35680-1.Peer-Reviewed Original ResearchConceptsG alpha 11G alpha 16G alpha qG alpha 14Alpha 14Phospholipase C beta 1C beta 1Alpha 11Alpha qBeta 1C receptorAlpha 16Cos-7 cellsPhospholipase CAlpha 1Subtypes of alphaRegulatory protein alpha subunitFunctional couplingG alpha subunitsAlpha subunitInositol phosphate releaseG alpha q/G alpha 11Synergistic responseReceptor subtypesSame assay systemActivation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells.
Wu D, Lee C, Rhee S, Simon M. Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells. Journal Of Biological Chemistry 1992, 267: 1811-1817. PMID: 1309799, DOI: 10.1016/s0021-9258(18)46018-8.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesCell LineCell MembraneEnzyme ActivationGTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)InositolInositol PhosphatesIsoenzymesKineticsMacromolecular SubstancesMolecular Sequence DataMutagenesis, Site-DirectedPeptidesPhosphatidylinositol Diacylglycerol-LyasePhosphoinositide Phospholipase CPhosphoric Diester HydrolasesTransfectionType C PhospholipasesConceptsG alpha 11G alpha qCos-7 cellsG alpha zAlpha qPI-PLCAlpha 11Alpha subunitAlpha zG protein alpha subunitsProtein alpha subunitsAlpha-subunit cDNAHigh steady-state levelsPhospholipase C beta 1Introduction of mutationsCell-free systemHigh-efficiency transient transfectionG protein activationC beta 1Glutamine 205Steady-state levelsBeta 1Transient transfectionProtein activationLeucine mutant