2020
The temporal effects of topical NF-κB inhibition, in the in vivo prevention of bile-related oncogenic mRNA and miRNA phenotypes in murine hypopharyngeal mucosa: a preclinical model
Vageli DP, Kasle D, Doukas SG, Doukas PG, Sasaki CT. The temporal effects of topical NF-κB inhibition, in the in vivo prevention of bile-related oncogenic mRNA and miRNA phenotypes in murine hypopharyngeal mucosa: a preclinical model. Oncotarget 2020, 11: 3303-3314. PMID: 32934775, PMCID: PMC7476734, DOI: 10.18632/oncotarget.27706.Peer-Reviewed Original ResearchNF-κB inhibitorBAY 11NF-κB-related pathwaysPharmacological NF-κB inhibitorSquamous cell cancerPro-inflammatory effectsNF-κB inhibitionNF-κB activationOncogenic mRNAsAcidic bileTopical preBile refluxHypopharyngeal cancerCell cancerPreclinical modelsTopical administrationHypopharyngeal mucosaBile exposureVivo preventionTranslational explorationMiR-155MiR-21Oncogenic eventsMiRNA phenotypesPrevents overexpressionThe in vivo preventive and therapeutic properties of curcumin in bile reflux‐related oncogenesis of the hypopharynx
Doukas SG, Doukas PG, Sasaki CT, Vageli D. The in vivo preventive and therapeutic properties of curcumin in bile reflux‐related oncogenesis of the hypopharynx. Journal Of Cellular And Molecular Medicine 2020, 24: 10311-10321. PMID: 32691972, PMCID: PMC7521262, DOI: 10.1111/jcmm.15640.Peer-Reviewed Original ResearchConceptsNF-κBRecent pre-clinical studiesHuman hypopharyngeal cellsIndependent risk factorPre-clinical studiesNF-κB activationTherapeutic propertiesHypopharyngeal cellsRisk factorsMolecular eventsHypopharyngeal mucosaBile exposureImmunohistochemical analysisFuture translational developmentBile acidsOncogenic profileHypopharynxCarcinogenic effectsOncogenic functionOverexpression of RelAEpithelial cellsBileTranslational developmentExpression of genesCurcuminThe Progressive Mutagenic Effects of Acidic Bile Refluxate in Hypopharyngeal Squamous Cell Carcinogenesis: New Insights
Sasaki CT, Doukas SG, Costa J, Vageli DP. The Progressive Mutagenic Effects of Acidic Bile Refluxate in Hypopharyngeal Squamous Cell Carcinogenesis: New Insights. Cancers 2020, 12: 1064. PMID: 32344873, PMCID: PMC7281001, DOI: 10.3390/cancers12051064.Peer-Reviewed Original ResearchAcidic bileUpper aerodigestive tractAdditional risk factorsBiomarkers of progressionSquamous cell carcinogenesisNF-κB activationNeck malignanciesAerodigestive tractInvasive cancerHistopathological changesRisk factorsHypopharyngeal mucosaEarly neoplasiaMalignant lesionsP53 expressionTranslational studiesVivo modelCell carcinogenesisBileExperimental modelEarly detectionCancerNovel evidenceMutagenic effectsFirst study
2016
Gastro-duodenal fluid induced nuclear factor- κappa B activation and early pre-malignant alterations in murine hypopharyngeal mucosa
Vageli DP, Prasad ML, Sasaki CT. Gastro-duodenal fluid induced nuclear factor- κappa B activation and early pre-malignant alterations in murine hypopharyngeal mucosa. Oncotarget 2016, 7: 5892-5908. PMID: 26745676, PMCID: PMC4868729, DOI: 10.18632/oncotarget.6824.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile Acids and SaltsBlotting, WesternCells, CulturedDuodenumFluorescent Antibody TechniqueHumansHypopharyngeal NeoplasmsHypopharynxImmunoenzyme TechniquesMiceMice, Inbred C57BLMucous MembraneNF-kappa BPrecancerous ConditionsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStomachConceptsHypopharyngeal mucosaNF-κB activationVivo modelSignificant NF-κB activationNF-κB pathwayCell proliferation markersΒ-catenin expressionC57BL/6J miceRisk factorsHistologic lesionsCell adhesion moleculeNF-κBVivo effectsProliferation markersP-STAT3B activationAdhesion moleculesNormal keratinocytesOncogenic pathwaysSTAT3 activationΒ-cateninBcl-2E-cadherinKi67Mucosa