2022
Noxious Combination of Tobacco Smoke Nitrosamines with Bile, Deoxycholic Acid, Promotes Hypopharyngeal Squamous Cell Carcinoma, via NFκB, In Vivo.
Vageli DP, Doukas PG, Doukas SG, Tsatsakis A, Judson BL. Noxious Combination of Tobacco Smoke Nitrosamines with Bile, Deoxycholic Acid, Promotes Hypopharyngeal Squamous Cell Carcinoma, via NFκB, In Vivo. Cancer Prevention Research 2022, 15: 297-308. PMID: 35502554, DOI: 10.1158/1940-6207.capr-21-0529.Peer-Reviewed Original ResearchConceptsHypopharyngeal squamous cell carcinomaTobacco smoke componentsSquamous cell carcinomaAerodigestive tract carcinogenesisBile refluxRisk factorsHypopharyngeal cancerCell carcinomaChronic exposureNFκB activationVivo modelUpper aerodigestive tract cancerNoxious combinationLarge-scale studiesIndependent risk factorAerodigestive tract cancerUpper aerodigestive tract carcinogenesisHigh-risk individualsVivo chronic exposurePresence of bileSilent progressionTract cancerTobacco smokingInvasive carcinomaPremalignant lesions
2020
The Progressive Mutagenic Effects of Acidic Bile Refluxate in Hypopharyngeal Squamous Cell Carcinogenesis: New Insights
Sasaki CT, Doukas SG, Costa J, Vageli DP. The Progressive Mutagenic Effects of Acidic Bile Refluxate in Hypopharyngeal Squamous Cell Carcinogenesis: New Insights. Cancers 2020, 12: 1064. PMID: 32344873, PMCID: PMC7281001, DOI: 10.3390/cancers12051064.Peer-Reviewed Original ResearchAcidic bileUpper aerodigestive tractAdditional risk factorsBiomarkers of progressionSquamous cell carcinogenesisNF-κB activationNeck malignanciesAerodigestive tractInvasive cancerHistopathological changesRisk factorsHypopharyngeal mucosaEarly neoplasiaMalignant lesionsP53 expressionTranslational studiesVivo modelCell carcinogenesisBileExperimental modelEarly detectionCancerNovel evidenceMutagenic effectsFirst study
2016
Gastro-duodenal fluid induced nuclear factor- κappa B activation and early pre-malignant alterations in murine hypopharyngeal mucosa
Vageli DP, Prasad ML, Sasaki CT. Gastro-duodenal fluid induced nuclear factor- κappa B activation and early pre-malignant alterations in murine hypopharyngeal mucosa. Oncotarget 2016, 7: 5892-5908. PMID: 26745676, PMCID: PMC4868729, DOI: 10.18632/oncotarget.6824.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile Acids and SaltsBlotting, WesternCells, CulturedDuodenumFluorescent Antibody TechniqueHumansHypopharyngeal NeoplasmsHypopharynxImmunoenzyme TechniquesMiceMice, Inbred C57BLMucous MembraneNF-kappa BPrecancerous ConditionsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStomachConceptsHypopharyngeal mucosaNF-κB activationVivo modelSignificant NF-κB activationNF-κB pathwayCell proliferation markersΒ-catenin expressionC57BL/6J miceRisk factorsHistologic lesionsCell adhesion moleculeNF-κBVivo effectsProliferation markersP-STAT3B activationAdhesion moleculesNormal keratinocytesOncogenic pathwaysSTAT3 activationΒ-cateninBcl-2E-cadherinKi67Mucosa