2021
Pepsin Promotes Activation of Epidermal Growth Factor Receptor and Downstream Oncogenic Pathways, at Slightly Acidic and Neutral pH, in Exposed Hypopharyngeal Cells
Doukas PG, Vageli DP, Sasaki CT, Judson BL. Pepsin Promotes Activation of Epidermal Growth Factor Receptor and Downstream Oncogenic Pathways, at Slightly Acidic and Neutral pH, in Exposed Hypopharyngeal Cells. International Journal Of Molecular Sciences 2021, 22: 4275. PMID: 33924087, PMCID: PMC8074291, DOI: 10.3390/ijms22084275.Peer-Reviewed Original ResearchConceptsRelated-signaling pathwaysOncogenic pathwaysLaryngopharyngeal reflux diseaseEpidermal growth factor receptorSpecific oncogenic pathwaysDownstream oncogenic pathwaysGrowth factor receptorHypopharyngeal cellsReflux diseaseRisk factorsTherapeutic approachesIntermittent exposureTumorigenic effectsPronounced activationOncogenic factorSignificant upregulationFactor receptorSTAT3 activationAcidic pepsinEGFR activationCell viabilityPromotes ActivationActivationPrimary cellsCells
2016
Gastro-duodenal fluid induced nuclear factor- κappa B activation and early pre-malignant alterations in murine hypopharyngeal mucosa
Vageli DP, Prasad ML, Sasaki CT. Gastro-duodenal fluid induced nuclear factor- κappa B activation and early pre-malignant alterations in murine hypopharyngeal mucosa. Oncotarget 2016, 7: 5892-5908. PMID: 26745676, PMCID: PMC4868729, DOI: 10.18632/oncotarget.6824.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile Acids and SaltsBlotting, WesternCells, CulturedDuodenumFluorescent Antibody TechniqueHumansHypopharyngeal NeoplasmsHypopharynxImmunoenzyme TechniquesMiceMice, Inbred C57BLMucous MembraneNF-kappa BPrecancerous ConditionsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStomachConceptsHypopharyngeal mucosaNF-κB activationVivo modelSignificant NF-κB activationNF-κB pathwayCell proliferation markersΒ-catenin expressionC57BL/6J miceRisk factorsHistologic lesionsCell adhesion moleculeNF-κBVivo effectsProliferation markersP-STAT3B activationAdhesion moleculesNormal keratinocytesOncogenic pathwaysSTAT3 activationΒ-cateninBcl-2E-cadherinKi67Mucosa