2022
Noxious Combination of Tobacco Smoke Nitrosamines with Bile, Deoxycholic Acid, Promotes Hypopharyngeal Squamous Cell Carcinoma, via NFκB, In Vivo.
Vageli DP, Doukas PG, Doukas SG, Tsatsakis A, Judson BL. Noxious Combination of Tobacco Smoke Nitrosamines with Bile, Deoxycholic Acid, Promotes Hypopharyngeal Squamous Cell Carcinoma, via NFκB, In Vivo. Cancer Prevention Research 2022, 15: 297-308. PMID: 35502554, DOI: 10.1158/1940-6207.capr-21-0529.Peer-Reviewed Original ResearchConceptsHypopharyngeal squamous cell carcinomaTobacco smoke componentsSquamous cell carcinomaAerodigestive tract carcinogenesisBile refluxRisk factorsHypopharyngeal cancerCell carcinomaChronic exposureNFκB activationVivo modelUpper aerodigestive tract cancerNoxious combinationLarge-scale studiesIndependent risk factorAerodigestive tract cancerUpper aerodigestive tract carcinogenesisHigh-risk individualsVivo chronic exposurePresence of bileSilent progressionTract cancerTobacco smokingInvasive carcinomaPremalignant lesions
2021
Bile reflux and hypopharyngeal cancer
Vageli DP, Doukas SG, Doukas PG, Judson BL. Bile reflux and hypopharyngeal cancer. Oncology Reports 2021, 46: 244. PMID: 34558652, PMCID: PMC8485019, DOI: 10.3892/or.2021.8195.Peer-Reviewed Original ResearchConceptsHypopharyngeal cancerAcidic bileHypopharyngeal carcinogenesisBile refluxRisk factorsGastroesophageal reflux diseaseIndependent risk factorSquamous cell cancerUpper aerodigestive tractElevated p53 expressionAnti-apoptotic effectsReflux diseaseLaryngopharyngeal refluxAerodigestive tractCell cancerGastroesophageal refluxateContribution of STAT3Neck cancerPrecancerous lesionsPremalignant changesNeoplastic changesNF-κBDNA damageP53 expressionBile acids
2019
Biliary reflux as a causal factor in hypopharyngeal carcinoma: New clinical evidence and implications
Sasaki CT, Doukas SG, Costa J, Vageli DP. Biliary reflux as a causal factor in hypopharyngeal carcinoma: New clinical evidence and implications. Cancer 2019, 125: 3554-3565. PMID: 31310330, DOI: 10.1002/cncr.32369.Peer-Reviewed Original ResearchConceptsHuman hypopharyngeal squamous cell carcinomaAdjacent normal tissuesIL-6Reflux diseaseHypopharyngeal squamous cell carcinomaMiR-375Respective adjacent normal tissuesMiR-21Pilot studyEsophageal reflux diseaseIndependent risk factorNew clinical evidenceSquamous cell carcinomaOncomiR miR-21NF-κB activationMiR-21/miRTumor suppressor miR-375Quantitative polymerase chain reactionMessenger RNABiliary refluxHypopharyngeal carcinogenesisControl patientsLaryngopharyngeal refluxClinical evidenceHypopharyngeal carcinoma
2016
Gastro-duodenal fluid induced nuclear factor- κappa B activation and early pre-malignant alterations in murine hypopharyngeal mucosa
Vageli DP, Prasad ML, Sasaki CT. Gastro-duodenal fluid induced nuclear factor- κappa B activation and early pre-malignant alterations in murine hypopharyngeal mucosa. Oncotarget 2016, 7: 5892-5908. PMID: 26745676, PMCID: PMC4868729, DOI: 10.18632/oncotarget.6824.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile Acids and SaltsBlotting, WesternCells, CulturedDuodenumFluorescent Antibody TechniqueHumansHypopharyngeal NeoplasmsHypopharynxImmunoenzyme TechniquesMiceMice, Inbred C57BLMucous MembraneNF-kappa BPrecancerous ConditionsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStomachConceptsHypopharyngeal mucosaNF-κB activationVivo modelSignificant NF-κB activationNF-κB pathwayCell proliferation markersΒ-catenin expressionC57BL/6J miceRisk factorsHistologic lesionsCell adhesion moleculeNF-κBVivo effectsProliferation markersP-STAT3B activationAdhesion moleculesNormal keratinocytesOncogenic pathwaysSTAT3 activationΒ-cateninBcl-2E-cadherinKi67Mucosa