1999
A Method for Determining Transmembrane Helix Association and Orientation in Detergent Micelles Using Small Angle X-Ray Scattering
Bu Z, Engelman D. A Method for Determining Transmembrane Helix Association and Orientation in Detergent Micelles Using Small Angle X-Ray Scattering. Biophysical Journal 1999, 77: 1064-1073. PMID: 10423450, PMCID: PMC1300396, DOI: 10.1016/s0006-3495(99)76956-0.Peer-Reviewed Original ResearchMeSH KeywordsBiophysical PhenomenaBiophysicsButyratesDetergentsDimerizationElectrochemistryGlycophorinsHumansIn Vitro TechniquesMembrane ProteinsMicellesMolecular WeightMutationProtein ConformationProtein Structure, SecondaryQuaternary Ammonium CompoundsRecombinant Fusion ProteinsScattering, RadiationSolutionsSolventsX-RaysConceptsDetergent micellesTransmembrane domainAlpha-helical transmembrane domainsSolution small-angle X-ray scatteringTransmembrane helix associationSolution small-angle X-rayHuman erythrocyte glycophorin ASmall-angle X-ray scatteringMembrane proteinsTransmembrane proteinErythrocyte glycophorin ACarboxyl terminusHelix associationAngle X-ray scatteringGlycophorin AStaphylococcal nucleaseSmall-angle X-rayProteinModel systemMicelle contributionX-ray scatteringAngle X-rayDimerizationGyration analysisN-dodecyl
1992
Forces involved in the assembly and stabilization of membrane proteins1
Cramer W, Engelman D, Von Heijne G, Rees D. Forces involved in the assembly and stabilization of membrane proteins1. The FASEB Journal 1992, 6: 3397-3402. PMID: 1464373, DOI: 10.1096/fasebj.6.15.1464373.Peer-Reviewed Original ResearchMeSH KeywordsCrystallizationElectrochemistryMembrane ProteinsPhotosynthetic Reaction Center Complex ProteinsProtein ConformationConceptsIntegral membrane proteinsHelix-helix interactionsMembrane proteinsIntegral membrane protein complexMembrane protein complexesMembrane-spanning domainsBacterial inner membraneSpecific dimer formationCytochrome b6f complexPolarity of residuesChloroplast thylakoid membranesWater-soluble proteinsProtein complexesReconstitution of bacteriorhodopsinHydrophobic subunitsInner membraneBacterial porinsB6f complexThylakoid membranesSuch polypeptidesBasic residuesProton translocationTrans sidePhotosynthetic reaction centersTrans-membrane orientation