2023
Plant MDL proteins synergize with the cytokine MIF at CXCR2 and CXCR4 receptors in human cells
Spiller L, Manjula R, Leissing F, Basquin J, Bourilhon P, Sinitski D, Brandhofer M, Levecque S, Gerra S, Sabelleck B, Zhang L, Feederle R, Flatley A, Hoffmann A, Panstruga R, Bernhagen J, Lolis E. Plant MDL proteins synergize with the cytokine MIF at CXCR2 and CXCR4 receptors in human cells. Science Signaling 2023, 16: eadg2621. PMID: 37988455, DOI: 10.1126/scisignal.adg2621.Peer-Reviewed Original ResearchConceptsMammalian macrophage migration inhibitory factorHetero-oligomeric complexesHigh structural similarityMultifunctional inflammatory cytokineHuman lung epithelial cellsYeast reporter systemReporter systemLung epithelial cellsPlant leavesFunctional similarityCellular responsesHuman cellsPharmacological inhibitorsDopachrome tautomeraseFunctional implicationsX-ray crystallographyMacrophage migration inhibitory factorStructural similarityEpithelial cellsMigration inhibitory factorCXCR4 receptorProteinTautomerase activityCellsMIF receptor
2002
Aggregation of Human Wild-Type and H27A-Prolactin in Cells and in Solution: Roles of Zn2+, Cu2+, and pH
Sankoorikal B, Zhu Y, Hodsdon M, Lolis E, Dannies P. Aggregation of Human Wild-Type and H27A-Prolactin in Cells and in Solution: Roles of Zn2+, Cu2+, and pH. Endocrinology 2002, 143: 1302-1309. DOI: 10.1210/en.143.4.1302.Peer-Reviewed Original ResearchAtT20 cellsSecretory granulesFormation of secretory granulesHuman prolactinZn2+-binding siteTransfected AtT20 cellsCores of secretory granulesHuman wild-typeWild-type prolactinAcidic pHCOS cellsRecombinant human prolactinIntracellular compartmentsWild-typeBinding sitesRat prolactinCu2+Neutral pHProlactinRatio of Zn2Concentrations of hormonesAtT20Cells