2020
Mitochondrial DNA content decreases during in vitro human embryo development: insights into mitochondrial DNA variation in preimplantation embryos donated for research
Pérez-Sánchez M, Díez-Juan A, Beltrán D, Mifsud A, Mercader A, Vidal C, Labarta E, Pellicer A, Seli E, De Los Santos MJ. Mitochondrial DNA content decreases during in vitro human embryo development: insights into mitochondrial DNA variation in preimplantation embryos donated for research. F&S Science 2020, 1: 36-45. PMID: 35559739, DOI: 10.1016/j.xfss.2020.05.001.Peer-Reviewed Original ResearchDay 3 embryosMetaphase II oocytesMtDNA contentII oocytesFailed-fertilized oocytesMtDNA copy numberProspective cohort studySignificant decreaseHuman metaphase II oocytesQuantitative polymerase chain reactionCohort studyFertilization patientsDNA loadPolymerase chain reactionPreimplantation embryo developmentAneuploid blastocystsOocyte donorsTotal mtDNA copy numberHuman oocytesEmbryo culture conditionsHuman embryo developmentCategorical variablesEmbryo developmentChain reaction
2013
Cumulus and granulosa cell markers of oocyte and embryo quality
Uyar A, Torrealday S, Seli E. Cumulus and granulosa cell markers of oocyte and embryo quality. Fertility And Sterility 2013, 99: 979-997. PMID: 23498999, PMCID: PMC3866131, DOI: 10.1016/j.fertnstert.2013.01.129.Peer-Reviewed Original ResearchConceptsQuantitative reverse transcriptase-polymerase chain reactionGranulosa cell gene expressionRelevant outcome parametersReverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionMural granulosa cellsGranulosa cell markersBiomarkers of oocyteClinical benefitOutcome parametersWhole genome expression profilingGranulosa cellsOocyte qualityEmbryo qualityCell markersFollicular cellsNoninvasive meansCell gene expressionGenome expression profilingChain reactionEmbryo competenceMultitude of studiesOocytesTranscriptomic profilingExpression profiling