2007
Estrogen increases apoptosis in the arterial wall in a murine atherosclerosis model
Seli E, Guzeloglu-Kayisli O, Kayisli UA, Kizilay G, Arici A. Estrogen increases apoptosis in the arterial wall in a murine atherosclerosis model. Fertility And Sterility 2007, 88: 1190-1196. PMID: 17498707, DOI: 10.1016/j.fertnstert.2007.01.132.Peer-Reviewed Original ResearchConceptsHigh-cholesterol dietMurine atherosclerosis modelAtherosclerotic plaque formationCholesterol dietFemale LDLAtherosclerosis modelPlaque formationArterial wallPlacebo-treated miceMitotic activityEffects of estrogenEffects of ovariectomyAtherosclerotic plaque developmentVascular effectsFemale miceMAIN OUTCOMEVascular apoptosisC57BL/6 backgroundPlaque developmentAortic wallLDLLDL receptorMice resultsMiceDNA strand breaks
2005
The significance of sperm nuclear DNA strand breaks on reproductive outcome
Spano M, Seli E, Bizzaro D, Manicardi GC, Sakkas D. The significance of sperm nuclear DNA strand breaks on reproductive outcome. Current Opinion In Obstetrics & Gynecology 2005, 17: 255-260. PMID: 15870559, DOI: 10.1097/01.gco.0000169102.77504.66.Peer-Reviewed Original ResearchConceptsNuclear DNA strand breaksDNA strand breaksStrand breaksDNA abnormalitiesPaternal genomeChromatin structureNuclear DNASperm nuclear DNASperm nucleiSperm chromatin structureNuclear abnormalitiesReproductive parametersBlastocyst developmentSpermatozoaBody of evidenceNuclear anomaliesGenomeDNA anomaliesBreaksDNAReproductionSpermatogenesisAssisted reproduction technologyFertilizationReproductive outcomesSpermatozoal nuclear determinants of reproductive outcome: implications for ART
Seli E, Sakkas D. Spermatozoal nuclear determinants of reproductive outcome: implications for ART. Human Reproduction Update 2005, 11: 337-349. PMID: 15863434, DOI: 10.1093/humupd/dmi011.Peer-Reviewed Original ResearchConceptsPaternal genomeEpigenetic regulationChromosome contentDNA strand breaksNuclear determinantsStrand breaksGenomeImportant functionsNuclear factorRecent findingsSpecific mechanismsReproductive outcomesExact mechanismNumerical abnormalitiesAssisted reproduction technology (ART) outcomesTreatment addressesReproductionMale factor infertilityY chromosome microdeletionsMale fertility potentialRegulationOocytesMechanismChromosome microdeletionsFactor infertility
2004
Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization
Seli E, Gardner DK, Schoolcraft WB, Moffatt O, Sakkas D. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertility And Sterility 2004, 82: 378-383. PMID: 15302287, DOI: 10.1016/j.fertnstert.2003.12.039.Peer-Reviewed Original ResearchConceptsBlastocyst developmentInfertility treatmentPregnancy rateTUNEL positivityIVF clinicsDNA strand breaksPrivate IVF clinicClinical pregnancy rateProspective comparative studyNick end labeling techniqueBlastocyst development ratePregnancy outcomesVs. 44MAIN OUTCOMESperm samplesLarge seriesSignificant negative correlationStrand breaksFlow cytometryTUNEL reactivityEnd-labeling techniqueIVFApoptotic cell deathPositivityNuclear DNA fragmentationThe presence of abnormal spermatozoa in the ejaculate: Did apoptosis fail?
Sakkas D, Seli E, Manicardi GC, Nijs M, Ombelet W, Bizzaro D. The presence of abnormal spermatozoa in the ejaculate: Did apoptosis fail? Human Fertility 2004, 7: 99-103. PMID: 15223758, DOI: 10.1080/14647270410001720464.Peer-Reviewed Original ResearchConceptsIntracytoplasmic sperm injectionNuclear DNA strand breaksDNA strand breaksAbnormal semen parametersAbnormal spermatozoaInfertile couplesMale infertilitySperm injectionSemen parametersQuality of spermatozoaAssisted reproductionStrand breaksCertain causesHuman spermatozoaSuch spermApoptosisSuccessful useNumerous studiesSpermatozoaPatients
2003
Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodelling during spermatogenesis
Sakkas D, Seli E, Bizzaro D, Tarozzi N, Manicardi GC. Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodelling during spermatogenesis. Reproductive BioMedicine Online 2003, 7: 428-432. PMID: 14656403, DOI: 10.1016/s1472-6483(10)61886-x.Peer-Reviewed Original ResearchConceptsNuclear DNA strand breaksDNA strand breaksAbnormal semen parametersAbnormal spermatozoaMale infertilitySemen parametersAbnormal persistenceApoptotic marker proteinsStrand breaksCertain causesHuman spermatozoaMarker proteinsApoptosisNumerous studiesSpermatogenesisSpermatozoaHumansInfertilityAbnormalities