2024
Non-invasive preimplantation genetic testing for aneuploidy: is the promise real?
Volovsky M, Scott R, Seli E. Non-invasive preimplantation genetic testing for aneuploidy: is the promise real? Human Reproduction 2024, 39: 1899-1908. PMID: 38970367, DOI: 10.1093/humrep/deae151.Peer-Reviewed Original ResearchNon-invasive PGT-APGT-APreimplantation genetic testingIntegration of next-generation sequencingGenetic testingGenetic testing platformsNon-invasive preimplantation genetic testingNext-generation sequencingRisk of pregnancy lossAneuploidy screening methodsEmbryo culture mediumAmplification failureSegmental aneuploidyPregnancy lossCulture mediumClinical reliabilityAneuploidyDiagnostic precisionClinical settingDiagnostic inaccuracyEmbryo viabilityClinical adoptionEmbryosScreening methodDNA
2021
Human embryo polarization requires PLC signaling to mediate trophectoderm specification
Zhu M, Shahbazi M, Martin A, Zhang C, Sozen B, Borsos M, Mandelbaum RS, Paulson RJ, Mole MA, Esbert M, Titus S, Scott RT, Campbell A, Fishel S, Gradinaru V, Zhao H, Wu K, Chen ZJ, Seli E, de los Santos MJ, Goetz M. Human embryo polarization requires PLC signaling to mediate trophectoderm specification. ELife 2021, 10: e65068. PMID: 34569938, PMCID: PMC8514238, DOI: 10.7554/elife.65068.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdultBody PatterningCell DifferentiationCell LineageCell PolarityEmbryo Culture TechniquesEmbryo, MammalianFemaleGATA3 Transcription FactorGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicHumansPhosphoinositide Phospholipase CPhospholipase C betaPregnancySignal TransductionTime FactorsYoung AdultConceptsApical domain formationInner cell massEmbryo polarizationPhospholipase CFirst lineage segregationApico-basal polarizationEight-cell stageLineage segregationMammalian developmentTrophectoderm specificationTE fateApical enrichmentApical accumulationDistinct lineagesApical domainApolar cellsPar complexPolarized cellsRNA interferenceMolecular mechanismsF-actinHuman embryosCell stageEmbryosDomain formationCumulus cells of euploid versus whole chromosome 21 aneuploid embryos reveal differentially expressed genes
Tiegs AW, Titus S, Mehta S, Garcia-Milian R, Seli E, Scott RT. Cumulus cells of euploid versus whole chromosome 21 aneuploid embryos reveal differentially expressed genes. Reproductive BioMedicine Online 2021, 43: 614-626. PMID: 34417138, DOI: 10.1016/j.rbmo.2021.06.015.Peer-Reviewed Original ResearchConceptsSerum response factorCumulus cellsDifferential gene expressionRNA sequencing analysisGene expression analysisIngenuity Pathway AnalysisCellular communication network factor 1Embryo developmental competenceExpression analysisPreimplantation embryo qualityGene expressionPathway analysisSegment polarity protein 2Sequencing analysisGenesProtein 2Response factorTrisomy 21Factor 1Developmental competenceAneuploid embryosReal-time polymerase chain reaction assaysDevelopment of biomarkersEmbryosCells
2019
Metabolic imaging via fluorescence lifetime imaging microscopy for egg and embryo assessment
Sanchez T, Zhang M, Needleman D, Seli E. Metabolic imaging via fluorescence lifetime imaging microscopy for egg and embryo assessment. Fertility And Sterility 2019, 111: 212-218. PMID: 30691624, DOI: 10.1016/j.fertnstert.2018.12.014.Peer-Reviewed Original ResearchConceptsMetabolic imagingInvasive diagnostic interventionsEmbryo assessmentPreimplantation genetic testingUseful diagnostic methodAssisted reproductive technology (ART) laboratoryClinical benefitSingle ETDiagnostic interventionsOxidative phosphorylationGenetic testingReproductive technology laboratoriesExperimental modelCopy number assessmentMetabolic functionsMorphologic parametersFluorescence lifetimeEmbryosClinical applicationMetabolic stateDiagnostic methodsEmbryo viabilityElectron transporterCentral roleCurrent strategies
2018
Mitochondrial unfolded protein response gene Clpp is required to maintain ovarian follicular reserve during aging, for oocyte competence, and development of pre‐implantation embryos
Wang T, Babayev E, Jiang Z, Li G, Zhang M, Esencan E, Horvath T, Seli E. Mitochondrial unfolded protein response gene Clpp is required to maintain ovarian follicular reserve during aging, for oocyte competence, and development of pre‐implantation embryos. Aging Cell 2018, 17: e12784. PMID: 29851234, PMCID: PMC6052477, DOI: 10.1111/acel.12784.Peer-Reviewed Original ResearchConceptsMitochondrial unfolded protein responseUnfolded mitochondrial proteinsCaseinolytic peptidase PAbsence of ClpPUnfolded protein responsePre-implantation embryosExpression of genesOocyte mitochondrial functionTwo-cell embryosProtein homeostasisMTOR inhibitor rapamycinMitochondrial proteinsOocyte competenceClpPProtein responseInhibitor rapamycinMitochondrial functionP-Akt473P-S6KOvarian follicular reserveSmall mitochondriaMTOR pathway activationPathway activationEmbryosP-S6
2013
Human embryonic poly(A)-binding protein (EPAB) alternative splicing is differentially regulated in human oocytes and embryos
Guzeloglu-Kayisli O, Lalioti MD, Babayev E, Torrealday S, Karakaya C, Seli E. Human embryonic poly(A)-binding protein (EPAB) alternative splicing is differentially regulated in human oocytes and embryos. Molecular Human Reproduction 2013, 20: 59-65. PMID: 24002949, DOI: 10.1093/molehr/gat061.Peer-Reviewed Original ResearchConceptsZygotic genome activationEarly embryo developmentEarly embryosTranslational activationSomatic tissuesSpliced formsEmbryo developmentEmbryo-specific expressionPost-transcriptional mechanismsOocyte maturationHuman somatic tissuesFull-length formGenome activationExon 8Transcriptional regulationAlternative splicingTranscriptional activityGene expressionHuman oocytesEPABAmino acidsXenopusEmbryosMRNAOocytes
2008
Identification and characterization of human embryonic poly(A) binding protein (EPAB)
Guzeloglu-Kayisli O, Pauli S, Demir H, Lalioti MD, Sakkas D, Seli E. Identification and characterization of human embryonic poly(A) binding protein (EPAB). Molecular Human Reproduction 2008, 14: 581-588. PMID: 18716053, DOI: 10.1093/molehr/gan047.Peer-Reviewed Original ResearchConceptsZygotic genome activationBinding proteinGene expressionInitial mitotic divisionsAmino acid proteinTranslational regulatory pathwaysEarly embryo developmentGenome activationModel organismsTranscriptional silencingMaternal mRNAsEarly embryosTranslational activationCytoplasmic polyadenylationSomatic tissuesAcid proteinRegulatory pathwaysMitotic divisionEmbryo developmentEPABProteinMRNA activationXenopusEmbryosCentral role