2024
Non-invasive preimplantation genetic testing for aneuploidy: is the promise real?
Volovsky M, Scott R, Seli E. Non-invasive preimplantation genetic testing for aneuploidy: is the promise real? Human Reproduction 2024, 39: 1899-1908. PMID: 38970367, DOI: 10.1093/humrep/deae151.Peer-Reviewed Original ResearchNon-invasive PGT-APGT-APreimplantation genetic testingIntegration of next-generation sequencingGenetic testingGenetic testing platformsNon-invasive preimplantation genetic testingNext-generation sequencingRisk of pregnancy lossAneuploidy screening methodsEmbryo culture mediumAmplification failureSegmental aneuploidyPregnancy lossCulture mediumClinical reliabilityAneuploidyDiagnostic precisionClinical settingDiagnostic inaccuracyEmbryo viabilityClinical adoptionEmbryosScreening methodDNALeukocytospermia does not negatively impact outcomes in in vitro fertilization cycles with intracytoplasmic sperm injection and preimplantation genetic testing for aneuploidy: findings from 5435 cycles
Gill P, Puchalt N, Molinaro T, Werner M, Seli E, Hotaling J, Cheng P. Leukocytospermia does not negatively impact outcomes in in vitro fertilization cycles with intracytoplasmic sperm injection and preimplantation genetic testing for aneuploidy: findings from 5435 cycles. Journal Of Assisted Reproduction And Genetics 2024, 41: 1213-1219. PMID: 38642270, PMCID: PMC11143111, DOI: 10.1007/s10815-024-03085-x.Peer-Reviewed Original ResearchConceptsLive birth rateIntracytoplasmic sperm injectionClinical pregnancy rateSustained implantation ratesIn vitro fertilizationIn vitro fertilization cyclesPreimplantation genetic testingBlastulation ratePGT-AAneuploidy rateEmbryological outcomesSperm injectionGenetic testingEuploid embryo transferPrevalence of leukocytospermiaRetrospective cohort studyMale partner ageImplantation rateBaseline characteristicsEmbryo transferLeukocytospermiaFertilization cyclesCohort studyPregnancy ratePrimary outcome
2021
Embryology outcomes after oocyte vitrification with super-cooled slush nitrogen are similar to outcomes with conventional liquid nitrogen: a randomized controlled trial
Hanson BM, Kim JG, Suarez SI, Ackerman BK, Comito CE, Pangasnan R, Seli E, Hong KH, Scott RT. Embryology outcomes after oocyte vitrification with super-cooled slush nitrogen are similar to outcomes with conventional liquid nitrogen: a randomized controlled trial. Fertility And Sterility 2021, 117: 106-114. PMID: 34654569, DOI: 10.1016/j.fertnstert.2021.08.043.Peer-Reviewed Original ResearchConceptsMetaphase II oocytesEmbryology outcomesOocyte survivalII oocytesOocyte donor cyclesOocyte survival ratePreimplantation genetic testingBlastocyst formationWhole chromosome aneuploidyChromosome aneuploidySecondary outcomesPrimary outcomeDonor cyclesDonor oocytesRate of fertilizationMAIN OUTCOMESurvival rateOocyte vitrificationTrophectoderm biopsyGenetic testingDonor spermPrivate practiceNext-generation sequencingOutcomesDemonstrable improvementIndividual culture leads to decreased blastocyst formation but does not affect pregnancy outcomes in the setting of a single, vitrified-warmed euploid blastocyst transfer
Glatthorn HN, Hanson BM, Kim JG, Herlihy NS, Klimczak AM, Hong KH, Seli E, Scott RT. Individual culture leads to decreased blastocyst formation but does not affect pregnancy outcomes in the setting of a single, vitrified-warmed euploid blastocyst transfer. Journal Of Assisted Reproduction And Genetics 2021, 38: 2157-2164. PMID: 34086147, PMCID: PMC8417170, DOI: 10.1007/s10815-021-02252-8.Peer-Reviewed Original ResearchConceptsEuploid blastocyst transferIntracytoplasmic sperm injectionPregnancy outcomesBlastocyst transferSignificant differencesBlastocyst formationSingle embryo transferMann-Whitney U testPreimplantation genetic testingLogistic regression modelsOvarian stimulationClinical miscarriageEuploid blastocystsSperm injectionEuploid embryosEmbryo transferTrophectoderm biopsyGenetic testingGroup embryo cultureU testSame time frameDecreased rateOutcomesMiscarriageFertilization rateNoninvasive prenatal testing in women undergoing in vitro fertilization with preimplantation genetic testing
Klimczak AM, Seli E, Scott RT. Noninvasive prenatal testing in women undergoing in vitro fertilization with preimplantation genetic testing. Current Opinion In Obstetrics & Gynecology 2021, 33: 184-187. PMID: 33797422, DOI: 10.1097/gco.0000000000000707.Peer-Reviewed Original ResearchConceptsPreimplantation genetic testingGenetic testingNoninvasive prenatal testing resultsPositive test resultsPrenatal screening toolPositive predictive valuePrenatal testing resultsPregnant womenEuploid embryosPredictive valueNoninvasive prenatal testingScreening toolWomenRecent evidenceIVFPrenatal testingPGTPregnancyPrevalencePrenatalTestingPreimplantation genetic testing to evaluate for mitochondrial deoxyribonucleic acid disease and aneuploidy: a two-birds-with-one-stone approach
Hanson BM, Seli E. Preimplantation genetic testing to evaluate for mitochondrial deoxyribonucleic acid disease and aneuploidy: a two-birds-with-one-stone approach. Fertility And Sterility 2021, 115: 1439-1440. PMID: 33795137, DOI: 10.1016/j.fertnstert.2021.03.006.Peer-Reviewed Original ResearchNoninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy
Hanson BM, Tao X, Hong KH, Comito CE, Pangasnan R, Seli E, Jalas C, Scott RT. Noninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy. Fertility And Sterility 2021, 115: 1461-1470. PMID: 33745720, DOI: 10.1016/j.fertnstert.2021.01.028.Peer-Reviewed Original ResearchMeSH KeywordsAneuploidyBlastocystCulture MediaEmbryo Culture TechniquesEmbryo ImplantationEmbryo TransferFemaleFertilityHigh-Throughput Nucleotide SequencingHumansInfertilityLive BirthMaleNoninvasive Prenatal TestingNucleic Acid Amplification TechniquesPredictive Value of TestsPregnancyPregnancy RatePreimplantation DiagnosisReproducibility of ResultsSperm Injections, IntracytoplasmicTime FactorsTreatment OutcomeWhole Genome SequencingConceptsPreimplantation genetic testingGenetic testingBiopsy resultsProspective cohort study SETTINGTrophectoderm biopsyTE biopsyNoninvasive preimplantation genetic testingCohort study SETTINGNext-generation sequencingHigh ratePrimary outcomeClinical outcomesMAIN OUTCOMEBiopsyStudy settingAmplification failureDiscordant resultsClinical applicabilityPrivate practiceNiPGTAvailable kitsCulture mediumShort durationOutcomesCulture medium samples
2020
Interpretation of noninvasive prenatal testing results following in vitro fertilization and preimplantation genetic testing for aneuploidy
Klimczak AM, Reig A, Neal SA, Seli E, Scott RT. Interpretation of noninvasive prenatal testing results following in vitro fertilization and preimplantation genetic testing for aneuploidy. American Journal Of Obstetrics & Gynecology MFM 2020, 2: 100232. PMID: 33345935, DOI: 10.1016/j.ajogmf.2020.100232.Peer-Reviewed Original ResearchConceptsPositive noninvasive prenatal testingPositive predictive valuePreimplantation genetic testingNoninvasive prenatal testing resultsEuploid embryosPredictive valueGeneral populationGenetic testingNoninvasive prenatal testingPrenatal testing resultsDiagnostic testingUniversity-affiliated fertility centrePrenatal testingRetrospective cohort studyLow-risk populationSingle euploid embryoPostnatal diagnostic testingCohort studyNormal babiesFertility centerPatient cohortPrenatal diagnostic testingRisk populationsLower riskPatientsA multicenter, prospective, blinded, nonselection study evaluating the predictive value of an aneuploid diagnosis using a targeted next-generation sequencing–based preimplantation genetic testing for aneuploidy assay and impact of biopsy
Tiegs AW, Tao X, Zhan Y, Whitehead C, Kim J, Hanson B, Osman E, Kim TJ, Patounakis G, Gutmann J, Castelbaum A, Seli E, Jalas C, Scott RT. A multicenter, prospective, blinded, nonselection study evaluating the predictive value of an aneuploid diagnosis using a targeted next-generation sequencing–based preimplantation genetic testing for aneuploidy assay and impact of biopsy. Fertility And Sterility 2020, 115: 627-637. PMID: 32863013, DOI: 10.1016/j.fertnstert.2020.07.052.Peer-Reviewed Original ResearchConceptsPreimplantation genetic testingSustained implantationPredictive valueGenetic testingClinical outcomesTrophectoderm biopsyAge-matched control groupImpact of biopsyPGT-A resultsAntral follicle countRecurrent pregnancy lossBody mass indexDetectable adverse impactAneuploid resultsSecondary outcomesFertilization cyclesFollicle countPrimary outcomeMass indexUsable blastocystsBlastocyst transferFertility centerPregnancy lossClinical error ratesStudy groupDevelopmental potential of aneuploid human embryos cultured beyond implantation
Shahbazi MN, Wang T, Tao X, Weatherbee BAT, Sun L, Zhan Y, Keller L, Smith GD, Pellicer A, Scott RT, Seli E, Zernicka-Goetz M. Developmental potential of aneuploid human embryos cultured beyond implantation. Nature Communications 2020, 11: 3987. PMID: 32778678, PMCID: PMC7418029, DOI: 10.1038/s41467-020-17764-7.Peer-Reviewed Original ResearchMeSH KeywordsAneuploidyAntigens, CDCadherinsCell AdhesionCell Cycle CheckpointsCell LineageChromosome SegregationChromosomes, Human, Pair 16Chromosomes, Human, Pair 21Embryo ImplantationEmbryo, MammalianEmbryonic DevelopmentFemaleGenes, erbB-1Genetic TestingHumansMonosomyMosaicismPregnancyStem CellsTrisomyConceptsEarly pregnancy lossAneuploid human embryosPre-implantation genetic testingHuman embryosPost-implantation developmentTrisomy 16 embryosPregnancy lossCell cycle arrestGenetic testingTrisomy 15Major causeCases of mosaicismCycle arrestE-cadherinTrisomy 21 embryosProtein E-cadherinHigh rateCommon aneuploidiesStem cellsPremature differentiationAbnormal numberAneuploidyCell adhesion protein E-cadherinDevelopmental potentialArrest
2019
Metabolic imaging via fluorescence lifetime imaging microscopy for egg and embryo assessment
Sanchez T, Zhang M, Needleman D, Seli E. Metabolic imaging via fluorescence lifetime imaging microscopy for egg and embryo assessment. Fertility And Sterility 2019, 111: 212-218. PMID: 30691624, DOI: 10.1016/j.fertnstert.2018.12.014.Peer-Reviewed Original ResearchConceptsMetabolic imagingInvasive diagnostic interventionsEmbryo assessmentPreimplantation genetic testingUseful diagnostic methodAssisted reproductive technology (ART) laboratoryClinical benefitSingle ETDiagnostic interventionsOxidative phosphorylationGenetic testingReproductive technology laboratoriesExperimental modelCopy number assessmentMetabolic functionsMorphologic parametersFluorescence lifetimeEmbryosClinical applicationMetabolic stateDiagnostic methodsEmbryo viabilityElectron transporterCentral roleCurrent strategies