2022
Distress response in granulosa cells of women affected by PCOS with or without insulin resistance
Cozzolino M, Herraiz S, Cakiroglu Y, Garcia-Velasco JA, Tiras B, Pacheco A, Rabadan S, Kohls G, Barrio AI, Pellicer A, Seli E. Distress response in granulosa cells of women affected by PCOS with or without insulin resistance. Endocrine 2022, 79: 200-207. PMID: 36149529, DOI: 10.1007/s12020-022-03192-8.Peer-Reviewed Original ResearchConceptsPolycystic ovarian syndromeHomeostasis model assessmentPCOS-IRInsulin resistanceHealthy oocyte donorsCumulus GCsOvarian syndromePCOS groupPlasma glucoseCONT groupMetabolic dysfunctionPathological changesFollicular microenvironmentConclusionOur findingsOocyte donorsGranulosa cellsModel assessmentQRT-PCRWomenER stressCONTMolecular mechanismsCell stressDistress responsesGroupTranscriptomic landscape of granulosa cells and peripheral blood mononuclear cells in women with PCOS compared to young poor responders and women with normal response
Cozzolino M, Herraiz S, Titus S, Roberts L, Romeu M, Peinado I, Scott RT, Pellicer A, Seli E. Transcriptomic landscape of granulosa cells and peripheral blood mononuclear cells in women with PCOS compared to young poor responders and women with normal response. Human Reproduction 2022, 37: 1274-1286. PMID: 35451009, PMCID: PMC9156844, DOI: 10.1093/humrep/deac069.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsYoung poor respondersPolycystic ovary syndromePoor ovarian responseGCs of PCOSOvarian granulosa cellsBlood mononuclear cellsGranulosa cellsMature folliclesPoor respondersMononuclear cellsRNA expression profilesNormal responseAge groupsSTUDY FUNDING/COMPETINGHigher menopausal ageRotterdam diagnostic criteriaOvarian hyperstimulation syndromeInstituto de Salud Carlos IIIPARTICIPANTS/MATERIALSAntral follicular countROLE OF CHANCEOvarian follicular dynamicsSame study groupIngenuity Pathway Analysis
2019
Cumulus cells have longer telomeres than leukocytes in reproductive-age women
Lara-Molina EE, Franasiak JM, Marin D, Tao X, Díaz-Gimeno P, Florensa M, Martin M, Seli E, Pellicer A. Cumulus cells have longer telomeres than leukocytes in reproductive-age women. Fertility And Sterility 2019, 113: 217-223. PMID: 31594634, DOI: 10.1016/j.fertnstert.2019.08.089.Peer-Reviewed Original ResearchConceptsLeukocyte telomere lengthGranulosa cellsCumulus cellsTelomere lengthAssisted reproductive technology centerRelative leukocyte telomere lengthProspective noninterventional studyAntimüllerian hormone levelsAntral follicle countReproductive-age womenReproductive technology centerLonger telomeresNoninterventional studyFollicle countMean ageHormone levelsFollicular environmentMAIN OUTCOMEMature folliclesStudy subjectsSignificant associationFollicular cellsMean numberMature oocytesSignificant differences
2016
Cross-Talk Between FSH and Endoplasmic Reticulum Stress: A Mutually Suppressive Relationship
Babayev E, Lalioti MD, Favero F, Seli E. Cross-Talk Between FSH and Endoplasmic Reticulum Stress: A Mutually Suppressive Relationship. Reproductive Sciences 2016, 23: 352-364. PMID: 26342052, PMCID: PMC5933091, DOI: 10.1177/1933719115602770.Peer-Reviewed Original ResearchConceptsFollicle stimulating hormonePregnant mare serum gonadotropinMouse granulosa cellsGranulosa cellsFSH responseSerum gonadotropinFSH stimulationER stressStress-induced cellsPrimary mouse granulosa cellsUntreated granulosa cellsMessenger RNA levelsCalcium adenosine triphosphataseEndoplasmic reticulum stressEstradiol levelsMice 24Estradiol productionIntraperitoneal injectionStimulating hormoneAromatase expressionTP treatmentReticulum stressRNA levelsER stress-associated genesProtein levels
2013
Characterization of the Gonadotropin Releasing Hormone Receptor (GnRHR) Expression and Activity in the Female Mouse Ovary
Torrealday S, Lalioti MD, Guzeloglu-Kayisli O, Seli E. Characterization of the Gonadotropin Releasing Hormone Receptor (GnRHR) Expression and Activity in the Female Mouse Ovary. Endocrinology 2013, 154: 3877-3887. PMID: 23913446, PMCID: PMC3776864, DOI: 10.1210/en.2013-1341.Peer-Reviewed Original ResearchConceptsGranulosa/cumulus cellsGranulosa cellsMouse ovariesReceptor expressionPrimary mouse granulosa cellsCumulus cellsPregnant mare serum gonadotropinIntracellular cAMPHormone receptor expressionGnRH receptor expressionCultured granulosa cellsOvarian granulosa cellsMouse granulosa cellsMechanism of actionGonadotoxic chemotherapyQuantitative RT-PCRGnRH agonistGnRHR mRNAFertility preservationMouse pituitary cellsLuciferase reporter plasmidSerum gonadotropinPituitary cell linePituitary cellsPituitary tissueCumulus and granulosa cell markers of oocyte and embryo quality
Uyar A, Torrealday S, Seli E. Cumulus and granulosa cell markers of oocyte and embryo quality. Fertility And Sterility 2013, 99: 979-997. PMID: 23498999, PMCID: PMC3866131, DOI: 10.1016/j.fertnstert.2013.01.129.Peer-Reviewed Original ResearchConceptsQuantitative reverse transcriptase-polymerase chain reactionGranulosa cell gene expressionRelevant outcome parametersReverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionMural granulosa cellsGranulosa cell markersBiomarkers of oocyteClinical benefitOutcome parametersWhole genome expression profilingGranulosa cellsOocyte qualityEmbryo qualityCell markersFollicular cellsNoninvasive meansCell gene expressionGenome expression profilingChain reactionEmbryo competenceMultitude of studiesOocytesTranscriptomic profilingExpression profiling