2021
Noninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy
Hanson BM, Tao X, Hong KH, Comito CE, Pangasnan R, Seli E, Jalas C, Scott RT. Noninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy. Fertility And Sterility 2021, 115: 1461-1470. PMID: 33745720, DOI: 10.1016/j.fertnstert.2021.01.028.Peer-Reviewed Original ResearchMeSH KeywordsAneuploidyBlastocystCulture MediaEmbryo Culture TechniquesEmbryo ImplantationEmbryo TransferFemaleFertilityHigh-Throughput Nucleotide SequencingHumansInfertilityLive BirthMaleNoninvasive Prenatal TestingNucleic Acid Amplification TechniquesPredictive Value of TestsPregnancyPregnancy RatePreimplantation DiagnosisReproducibility of ResultsSperm Injections, IntracytoplasmicTime FactorsTreatment OutcomeWhole Genome SequencingConceptsPreimplantation genetic testingGenetic testingBiopsy resultsProspective cohort study SETTINGTrophectoderm biopsyTE biopsyNoninvasive preimplantation genetic testingCohort study SETTINGNext-generation sequencingHigh ratePrimary outcomeClinical outcomesMAIN OUTCOMEBiopsyStudy settingAmplification failureDiscordant resultsClinical applicabilityPrivate practiceNiPGTAvailable kitsCulture mediumShort durationOutcomesCulture medium samples
2020
Shorter telomere length of white blood cells is associated with higher rates of aneuploidy among infertile women undergoing in vitro fertilization
Hanson BM, Tao X, Zhan Y, Kim JG, Klimczak AM, Herlihy NS, Scott RT, Seli E. Shorter telomere length of white blood cells is associated with higher rates of aneuploidy among infertile women undergoing in vitro fertilization. Fertility And Sterility 2020, 115: 957-965. PMID: 33272640, DOI: 10.1016/j.fertnstert.2020.09.164.Peer-Reviewed Original ResearchConceptsWhite blood cellsPatient ageInfertile womenCumulus cellsBlood cellsEmbryonic aneuploidyPeak estradiol levelsAntimüllerian hormone levelsProspective cohort studyTelomere lengthNumber of oocytesShorter telomere lengthReal-time polymerase chain reactionQuantitative real-time polymerase chain reactionHigh rateWarrants further investigationTelomere length assessmentSignificant overlap existsCohort studyOocyte retrievalSingle centerEstradiol levelsInfertile populationMaternal agePolymerase chain reactionDevelopmental potential of aneuploid human embryos cultured beyond implantation
Shahbazi MN, Wang T, Tao X, Weatherbee BAT, Sun L, Zhan Y, Keller L, Smith GD, Pellicer A, Scott RT, Seli E, Zernicka-Goetz M. Developmental potential of aneuploid human embryos cultured beyond implantation. Nature Communications 2020, 11: 3987. PMID: 32778678, PMCID: PMC7418029, DOI: 10.1038/s41467-020-17764-7.Peer-Reviewed Original ResearchMeSH KeywordsAneuploidyAntigens, CDCadherinsCell AdhesionCell Cycle CheckpointsCell LineageChromosome SegregationChromosomes, Human, Pair 16Chromosomes, Human, Pair 21Embryo ImplantationEmbryo, MammalianEmbryonic DevelopmentFemaleGenes, erbB-1Genetic TestingHumansMonosomyMosaicismPregnancyStem CellsTrisomyConceptsEarly pregnancy lossAneuploid human embryosPre-implantation genetic testingHuman embryosPost-implantation developmentTrisomy 16 embryosPregnancy lossCell cycle arrestGenetic testingTrisomy 15Major causeCases of mosaicismCycle arrestE-cadherinTrisomy 21 embryosProtein E-cadherinHigh rateCommon aneuploidiesStem cellsPremature differentiationAbnormal numberAneuploidyCell adhesion protein E-cadherinDevelopmental potentialArrest