2020
Mitochondrial DNA content decreases during in vitro human embryo development: insights into mitochondrial DNA variation in preimplantation embryos donated for research
Pérez-Sánchez M, Díez-Juan A, Beltrán D, Mifsud A, Mercader A, Vidal C, Labarta E, Pellicer A, Seli E, De Los Santos MJ. Mitochondrial DNA content decreases during in vitro human embryo development: insights into mitochondrial DNA variation in preimplantation embryos donated for research. F&S Science 2020, 1: 36-45. PMID: 35559739, DOI: 10.1016/j.xfss.2020.05.001.Peer-Reviewed Original ResearchDay 3 embryosMetaphase II oocytesMtDNA contentII oocytesFailed-fertilized oocytesMtDNA copy numberProspective cohort studySignificant decreaseHuman metaphase II oocytesQuantitative polymerase chain reactionCohort studyFertilization patientsDNA loadPolymerase chain reactionPreimplantation embryo developmentAneuploid blastocystsOocyte donorsTotal mtDNA copy numberHuman oocytesEmbryo culture conditionsHuman embryo developmentCategorical variablesEmbryo developmentChain reaction
2013
Human embryonic poly(A)-binding protein (EPAB) alternative splicing is differentially regulated in human oocytes and embryos
Guzeloglu-Kayisli O, Lalioti MD, Babayev E, Torrealday S, Karakaya C, Seli E. Human embryonic poly(A)-binding protein (EPAB) alternative splicing is differentially regulated in human oocytes and embryos. Molecular Human Reproduction 2013, 20: 59-65. PMID: 24002949, DOI: 10.1093/molehr/gat061.Peer-Reviewed Original ResearchConceptsZygotic genome activationEarly embryo developmentEarly embryosTranslational activationSomatic tissuesSpliced formsEmbryo developmentEmbryo-specific expressionPost-transcriptional mechanismsOocyte maturationHuman somatic tissuesFull-length formGenome activationExon 8Transcriptional regulationAlternative splicingTranscriptional activityGene expressionHuman oocytesEPABAmino acidsXenopusEmbryosMRNAOocytes