2021
Human embryo polarization requires PLC signaling to mediate trophectoderm specification
Zhu M, Shahbazi M, Martin A, Zhang C, Sozen B, Borsos M, Mandelbaum RS, Paulson RJ, Mole MA, Esbert M, Titus S, Scott RT, Campbell A, Fishel S, Gradinaru V, Zhao H, Wu K, Chen ZJ, Seli E, de los Santos MJ, Goetz M. Human embryo polarization requires PLC signaling to mediate trophectoderm specification. ELife 2021, 10: e65068. PMID: 34569938, PMCID: PMC8514238, DOI: 10.7554/elife.65068.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdultBody PatterningCell DifferentiationCell LineageCell PolarityEmbryo Culture TechniquesEmbryo, MammalianFemaleGATA3 Transcription FactorGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicHumansPhosphoinositide Phospholipase CPhospholipase C betaPregnancySignal TransductionTime FactorsYoung AdultConceptsApical domain formationInner cell massEmbryo polarizationPhospholipase CFirst lineage segregationApico-basal polarizationEight-cell stageLineage segregationMammalian developmentTrophectoderm specificationTE fateApical enrichmentApical accumulationDistinct lineagesApical domainApolar cellsPar complexPolarized cellsRNA interferenceMolecular mechanismsF-actinHuman embryosCell stageEmbryosDomain formation
2020
Analysis of accessible chromatin landscape in the inner cell mass and trophectoderm of human blastocysts
Yang M, Tao X, Titus S, Zhao T, Scott RT, Seli E. Analysis of accessible chromatin landscape in the inner cell mass and trophectoderm of human blastocysts. Molecular Human Reproduction 2020, 26: 702-711. PMID: 32663300, DOI: 10.1093/molehr/gaaa048.Peer-Reviewed Original ResearchConceptsInner cell massHuman preimplantation embryosEpigenetic regulationPreimplantation embryosCell lineage specificationChromatin accessibility landscapeAccessible chromatin landscapePreimplantation human blastocystsEarly embryonic developmentTranscription start siteCell massEarly embryo developmentHuman blastocystsDistal regionChromatin landscapeAccessible chromatinLineage specificationChromatin structureLow DNA inputATAC-seqTrophectoderm differentiationChromosome reorganizationAccessibility landscapeHuman embryonic materialEmbryonic development