2021
B-cell lymphoma 6 expression is not associated with live birth in a normal responder in vitro fertilization population
Klimczak AM, Herlihy NS, Scott CS, Hanson BM, Kim JG, Titus S, Seli E, Scott RT. B-cell lymphoma 6 expression is not associated with live birth in a normal responder in vitro fertilization population. Fertility And Sterility 2021, 117: 351-358. PMID: 34809978, DOI: 10.1016/j.fertnstert.2021.09.036.Peer-Reviewed Original ResearchConceptsB-cell lymphoma 6 (Bcl6) expressionLive birth groupLive birthsFertilization populationNormal respondersBirth groupProportion of patientsLive birth ratePopulation of patientsEuploid embryo transferCase-control studyEndometrial tissue samplesPreimplantation genetic testingGroup of womenIVF stimulationMean HSCOREIVF outcomesInfertility centerIVF successMAIN OUTCOMEGroup 2Group 1Embryo transferPatientsClinical decisionEmbryology outcomes after oocyte vitrification with super-cooled slush nitrogen are similar to outcomes with conventional liquid nitrogen: a randomized controlled trial
Hanson BM, Kim JG, Suarez SI, Ackerman BK, Comito CE, Pangasnan R, Seli E, Hong KH, Scott RT. Embryology outcomes after oocyte vitrification with super-cooled slush nitrogen are similar to outcomes with conventional liquid nitrogen: a randomized controlled trial. Fertility And Sterility 2021, 117: 106-114. PMID: 34654569, DOI: 10.1016/j.fertnstert.2021.08.043.Peer-Reviewed Original ResearchConceptsMetaphase II oocytesEmbryology outcomesOocyte survivalII oocytesOocyte donor cyclesOocyte survival ratePreimplantation genetic testingBlastocyst formationWhole chromosome aneuploidyChromosome aneuploidySecondary outcomesPrimary outcomeDonor cyclesDonor oocytesRate of fertilizationMAIN OUTCOMESurvival rateOocyte vitrificationTrophectoderm biopsyGenetic testingDonor spermPrivate practiceNext-generation sequencingOutcomesDemonstrable improvementNoninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy
Hanson BM, Tao X, Hong KH, Comito CE, Pangasnan R, Seli E, Jalas C, Scott RT. Noninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy. Fertility And Sterility 2021, 115: 1461-1470. PMID: 33745720, DOI: 10.1016/j.fertnstert.2021.01.028.Peer-Reviewed Original ResearchMeSH KeywordsAneuploidyBlastocystCulture MediaEmbryo Culture TechniquesEmbryo ImplantationEmbryo TransferFemaleFertilityHigh-Throughput Nucleotide SequencingHumansInfertilityLive BirthMaleNoninvasive Prenatal TestingNucleic Acid Amplification TechniquesPredictive Value of TestsPregnancyPregnancy RatePreimplantation DiagnosisReproducibility of ResultsSperm Injections, IntracytoplasmicTime FactorsTreatment OutcomeWhole Genome SequencingConceptsPreimplantation genetic testingGenetic testingBiopsy resultsProspective cohort study SETTINGTrophectoderm biopsyTE biopsyNoninvasive preimplantation genetic testingCohort study SETTINGNext-generation sequencingHigh ratePrimary outcomeClinical outcomesMAIN OUTCOMEBiopsyStudy settingAmplification failureDiscordant resultsClinical applicabilityPrivate practiceNiPGTAvailable kitsCulture mediumShort durationOutcomesCulture medium samples
2020
Rate of true recurrent implantation failure is low: results of three successive frozen euploid single embryo transfers
Pirtea P, De Ziegler D, Tao X, Sun L, Zhan Y, Ayoubi JM, Seli E, Franasiak JM, Scott RT. Rate of true recurrent implantation failure is low: results of three successive frozen euploid single embryo transfers. Fertility And Sterility 2020, 115: 45-53. PMID: 33077239, DOI: 10.1016/j.fertnstert.2020.07.002.Peer-Reviewed Original ResearchConceptsRecurrent implantation failureEuploid single-embryo transferLive birth rateSingle embryo transferImplantation rateImplantation failureEmbryo transferCumulative live birth rateSustained implantation ratesRetrospective cohort studyKaplan-Meier curvesReproductive technology centerDifference of outcomeBirth rateCumulative implantation rateLogistic regression modelsPositive heartbeatClinical pregnancyCohort studyMiscarriage rateMean ageEuploid blastocystsNormal uterusMAIN OUTCOMEGestational carrier
2019
Cumulus cells have longer telomeres than leukocytes in reproductive-age women
Lara-Molina EE, Franasiak JM, Marin D, Tao X, Díaz-Gimeno P, Florensa M, Martin M, Seli E, Pellicer A. Cumulus cells have longer telomeres than leukocytes in reproductive-age women. Fertility And Sterility 2019, 113: 217-223. PMID: 31594634, DOI: 10.1016/j.fertnstert.2019.08.089.Peer-Reviewed Original ResearchConceptsLeukocyte telomere lengthGranulosa cellsCumulus cellsTelomere lengthAssisted reproductive technology centerRelative leukocyte telomere lengthProspective noninterventional studyAntimüllerian hormone levelsAntral follicle countReproductive-age womenReproductive technology centerLonger telomeresNoninterventional studyFollicle countMean ageHormone levelsFollicular environmentMAIN OUTCOMEMature folliclesStudy subjectsSignificant associationFollicular cellsMean numberMature oocytesSignificant differences
2018
Metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes
Sanchez T, Wang T, Pedro MV, Zhang M, Esencan E, Sakkas D, Needleman D, Seli E. Metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes. Fertility And Sterility 2018, 110: 1387-1397. PMID: 30446247, PMCID: PMC6289735, DOI: 10.1016/j.fertnstert.2018.07.022.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedComputer SystemsEmbryo Culture TechniquesEmbryo, MammalianEmbryonic DevelopmentEndopeptidase ClpFemaleFlavin-Adenine DinucleotideFluorescenceMaleMaternal AgeMiceMice, Inbred C57BLMice, KnockoutMicroscopy, FluorescenceMitochondriaMolecular ImagingNADOocytesReactive Oxygen SpeciesConceptsBlastocyst development rateOocyte dysfunctionReactive oxygen species levelsFlavin adenine dinucleotide (FAD) autofluorescenceMetabolic dysfunctionOxygen species levelsYoung miceMetabolic parametersOld miceMAIN OUTCOMEGlobal knockoutDysfunctionNoninvasive toolNormal oocytesMetabolic imagingMitochondrial dysfunctionMiceOld oocytesFLIM parametersROS levelsMetabolic differencesMitochondrial functionNicotinamide adenine dinucleotide dehydrogenaseIndividual oocytesWild-type oocytes
2012
The association between fertility clinic performance and cycle volume: implications for public reporting of provider performance data
Gong D, Seli E. The association between fertility clinic performance and cycle volume: implications for public reporting of provider performance data. Fertility And Sterility 2012, 98: 55-62.e1. PMID: 22521157, DOI: 10.1016/j.fertnstert.2012.03.036.Peer-Reviewed Original ResearchConceptsLive birth rateProvider performance dataClinic performanceSuccess rateCycle volumeReproductive technology clinicsRetrospective longitudinal analysisHealth care providersBirth ratePublic reportingART cyclesPregnancy rateART treatmentStudy populationMAIN OUTCOMECare providersStratification analysisTechnology clinicsPatient behaviorClinicDisease controlPublic quality reportingPatientsProvider performanceLongitudinal analysis
2010
Gay men choosing parenthood through assisted reproduction: medical and psychosocial considerations
Greenfeld DA, Seli E. Gay men choosing parenthood through assisted reproduction: medical and psychosocial considerations. Fertility And Sterility 2010, 95: 225-229. PMID: 20643400, DOI: 10.1016/j.fertnstert.2010.05.053.Peer-Reviewed Original Research
2009
Noninvasive metabolomic profiling as an adjunct to morphology for noninvasive embryo assessment in women undergoing single embryo transfer
Seli E, Vergouw CG, Morita H, Botros L, Roos P, Lambalk CB, Yamashita N, Kato O, Sakkas D. Noninvasive metabolomic profiling as an adjunct to morphology for noninvasive embryo assessment in women undergoing single embryo transfer. Fertility And Sterility 2009, 94: 535-542. PMID: 19589524, DOI: 10.1016/j.fertnstert.2009.03.078.Peer-Reviewed Original ResearchConceptsSingle embryo transferDay 3 embryosDay 2Embryo transferImplantation rateEmbryo culture mediumMetabolomic profilingPositive fetal cardiac activityAssisted reproductive technology programViability indexNoninvasive metabolomic profilingFetal cardiac activityReproductive technology programHuman embryo culture mediaRetrospective studyMAIN OUTCOMEDay 3Morphology gradeBlinded analysisCardiac activityReproductive potentialEmbryo assessmentCulture mediumBlinded validationWomen
2008
Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization
Seli E, Botros L, Sakkas D, Burns DH. Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization. Fertility And Sterility 2008, 90: 2183-2189. PMID: 18842260, DOI: 10.1016/j.fertnstert.2008.07.1739.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersCulture MediaEmbryo Culture TechniquesEmbryo ImplantationEmbryo TransferEmbryo, MammalianEmbryonic DevelopmentFemaleFertilization in VitroHumansLive BirthMagnetic Resonance SpectroscopyMetabolomicsPredictive Value of TestsPregnancyPregnancy RateProtonsRetrospective StudiesSensitivity and SpecificityTreatment OutcomeConceptsEmbryo culture mediumNoninvasive metabolomic profilingMetabolomic profilesMetabolomic profilingReproductive technology programViability indexNuclear magnetic resonance metabolomic profilingRetrospective studyART treatmentMAIN OUTCOMEReproductive outcomesCulture mediumDay 3NMR metabolomic profilesGlutamate concentrationMultivariate analysisPregnancyNoninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study
Scott R, Seli E, Miller K, Sakkas D, Scott K, Burns DH. Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study. Fertility And Sterility 2008, 90: 77-83. PMID: 18281045, DOI: 10.1016/j.fertnstert.2007.11.058.Peer-Reviewed Original ResearchConceptsMetabolomic profilingProspective blinded pilot studyNoninvasive metabolomic profilingEmbryonic reproductive potentialBlinded pilot studyReproductive technology programCharacteristic curve analysisHuman embryo culture mediaOverall diagnostic accuracyViability indexImplantation rateEmbryo culture mediumMAIN OUTCOMEDay 3Noninvasive assessmentDay 5Culture mediumDiagnostic accuracyPilot studyCurve analysisSimilar findingsReproductive potentialRapid noninvasive assessmentDelivery rateHuman embryos
2007
Estrogen increases apoptosis in the arterial wall in a murine atherosclerosis model
Seli E, Guzeloglu-Kayisli O, Kayisli UA, Kizilay G, Arici A. Estrogen increases apoptosis in the arterial wall in a murine atherosclerosis model. Fertility And Sterility 2007, 88: 1190-1196. PMID: 17498707, DOI: 10.1016/j.fertnstert.2007.01.132.Peer-Reviewed Original ResearchConceptsHigh-cholesterol dietMurine atherosclerosis modelAtherosclerotic plaque formationCholesterol dietFemale LDLAtherosclerosis modelPlaque formationArterial wallPlacebo-treated miceMitotic activityEffects of estrogenEffects of ovariectomyAtherosclerotic plaque developmentVascular effectsFemale miceMAIN OUTCOMEVascular apoptosisC57BL/6 backgroundPlaque developmentAortic wallLDLLDL receptorMice resultsMiceDNA strand breaks
2006
Estradiol Increases Apoptosis in Human Coronary Artery Endothelial Cells by Up-Regulating Fas and Fas Ligand Expression
Seli E, Guzeloglu-Kayisli O, Cakmak H, Kayisli UA, Selam B, Arici A. Estradiol Increases Apoptosis in Human Coronary Artery Endothelial Cells by Up-Regulating Fas and Fas Ligand Expression. The Journal Of Clinical Endocrinology & Metabolism 2006, 91: 4995-5001. PMID: 17003091, DOI: 10.1210/jc.2006-1225.Peer-Reviewed Original ResearchConceptsHuman coronary artery endothelial cellsCultured human coronary artery endothelial cellsHormone replacement therapyCoronary artery endothelial cellsArtery endothelial cellsReplacement therapyE2 treatmentPlaque stabilityPostmenopausal hormone replacement therapyEndothelial cellsFas/FasL pathwayEffects of E2Effects of estrogenAtherosclerotic plaque formationSignificant concentration-dependent increaseConcentration-dependent increaseFas ligand expressionCardiovascular eventsCardioprotective effectsInhibits atherogenesisMAIN OUTCOMEAnimal modelsFasL pathwayUp-regulating FasLigand expression
2004
Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization
Seli E, Gardner DK, Schoolcraft WB, Moffatt O, Sakkas D. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertility And Sterility 2004, 82: 378-383. PMID: 15302287, DOI: 10.1016/j.fertnstert.2003.12.039.Peer-Reviewed Original ResearchConceptsBlastocyst developmentInfertility treatmentPregnancy rateTUNEL positivityIVF clinicsDNA strand breaksPrivate IVF clinicClinical pregnancy rateProspective comparative studyNick end labeling techniqueBlastocyst development ratePregnancy outcomesVs. 44MAIN OUTCOMESperm samplesLarge seriesSignificant negative correlationStrand breaksFlow cytometryTUNEL reactivityEnd-labeling techniqueIVFApoptotic cell deathPositivityNuclear DNA fragmentation
2002
Estradiol down-regulates MCP-1 expression in human coronary artery endothelial cells
Seli E, Pehlivan T, Selam B, Garcia-Velasco JA, Arici A. Estradiol down-regulates MCP-1 expression in human coronary artery endothelial cells. Fertility And Sterility 2002, 77: 542-547. PMID: 11872210, DOI: 10.1016/s0015-0282(01)03223-x.Peer-Reviewed Original ResearchConceptsHuman coronary artery endothelial cellsCoronary artery endothelial cellsMCP-1 expressionHuman umbilical vein endothelial cellsEnzyme-linked immunosorbent assayArtery endothelial cellsMCP-1 mRNAUmbilical vein endothelial cellsEndothelial cellsVein endothelial cellsMCP-1Prospective comparative studyConcentrations of estradiolMCP-1 productionMCP-1 proteinDose-dependent inhibitionVascular endothelial cellsEstrogen treatmentMacrophage recruitmentMAIN OUTCOMEMRNA expressionRibonucleic acidImmunosorbent assayProtein expressionTamoxifen