2012
Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice
Guzeloglu-Kayisli O, Lalioti MD, Aydiner F, Sasson I, Ilbay O, Sakkas D, Lowther KM, Mehlmann LM, Seli E. Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice. Biochemical Journal 2012, 446: 47-58. PMID: 22621333, PMCID: PMC3955213, DOI: 10.1042/bj20120467.Peer-Reviewed Original ResearchConceptsTranslational activationOocyte maturationZygotic genome activationFemale fertilityGenome activationGerminal vesicle stage oocytesDazl mRNAEarly embryogenesisMaternal mRNAsDownstream regulatorsMammalian reproductionGene expressionEPABStage oocytesXenopus oocytesEpidermal growthLate antral folliclesOogenesisMRNAOocytesMaturationProteinFemale miceCumulus expansionAntral folliclesEmbryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation
Friend K, Brook M, Bezirci FB, Sheets MD, Gray NK, Seli E. Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation. Biochemical Journal 2012, 445: 93-100. PMID: 22497250, PMCID: PMC3955212, DOI: 10.1042/bj20120304.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCytoplasmFemaleFluorescent Antibody TechniqueGene Expression Regulation, DevelopmentalImmunoprecipitationMutationOocytesOogenesisPhosphorylationPoly APoly(A)-Binding ProteinsPolyadenylationProtein BiosynthesisProtein Processing, Post-TranslationalRNA, MessengerXenopus laevisXenopus ProteinsConceptsCytoplasmic polyadenylationOocyte maturationPost-transcriptional regulatorsEarly embryonic developmentPost-translational modificationsXenopus oocyte maturationMaternal mRNAsProtein phosphorylationResidue clustersTranslational activationEmbryonic developmentXenopus laevis oocytesProtein activityPhosphorylationFirst insightLaevis oocytesPolyadenylationInherent abilityEPABMaturationPhosphoproteinRegulatorMutationsMRNAOocytes
2008
Identification and characterization of human embryonic poly(A) binding protein (EPAB)
Guzeloglu-Kayisli O, Pauli S, Demir H, Lalioti MD, Sakkas D, Seli E. Identification and characterization of human embryonic poly(A) binding protein (EPAB). Molecular Human Reproduction 2008, 14: 581-588. PMID: 18716053, DOI: 10.1093/molehr/gan047.Peer-Reviewed Original ResearchConceptsZygotic genome activationBinding proteinGene expressionInitial mitotic divisionsAmino acid proteinTranslational regulatory pathwaysEarly embryo developmentGenome activationModel organismsTranscriptional silencingMaternal mRNAsEarly embryosTranslational activationCytoplasmic polyadenylationSomatic tissuesAcid proteinRegulatory pathwaysMitotic divisionEmbryo developmentEPABProteinMRNA activationXenopusEmbryosCentral roleAlternative splicing of the mouse embryonic poly(A) binding protein (Epab) mRNA is regulated by an exonic splicing enhancer: a model for post-transcriptional control of gene expression in the oocyte
Seli E, Yaba A, Guzeloglu-Kayisli O, Lalioti MD. Alternative splicing of the mouse embryonic poly(A) binding protein (Epab) mRNA is regulated by an exonic splicing enhancer: a model for post-transcriptional control of gene expression in the oocyte. Molecular Human Reproduction 2008, 14: 393-398. PMID: 18492745, PMCID: PMC2453241, DOI: 10.1093/molehr/gan028.Peer-Reviewed Original ResearchConceptsExonic splicing enhancersAlternative splicing variantsSingle nucleotide polymorphismsAlternative splicingSplicing variantsSplicing enhancersExon 10Post-transcriptional controlMRNA alternative splicingEarly preimplantation embryosOocyte-specific proteinRNA editingMaternal mRNAsEarly embryosMouse embryonicBinding protein mRNAReal-time RT-PCR resultsGene expressionPreimplantation embryosSpliced formsSame locusSequence analysisBinding proteinSplicingParental origin