2009
Noninvasive metabolomic profiling as an adjunct to morphology for noninvasive embryo assessment in women undergoing single embryo transfer
Seli E, Vergouw CG, Morita H, Botros L, Roos P, Lambalk CB, Yamashita N, Kato O, Sakkas D. Noninvasive metabolomic profiling as an adjunct to morphology for noninvasive embryo assessment in women undergoing single embryo transfer. Fertility And Sterility 2009, 94: 535-542. PMID: 19589524, DOI: 10.1016/j.fertnstert.2009.03.078.Peer-Reviewed Original ResearchConceptsSingle embryo transferDay 3 embryosDay 2Embryo transferImplantation rateEmbryo culture mediumMetabolomic profilingPositive fetal cardiac activityAssisted reproductive technology programViability indexNoninvasive metabolomic profilingFetal cardiac activityReproductive technology programHuman embryo culture mediaRetrospective studyMAIN OUTCOMEDay 3Morphology gradeBlinded analysisCardiac activityReproductive potentialEmbryo assessmentCulture mediumBlinded validationWomen
2008
Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization
Seli E, Botros L, Sakkas D, Burns DH. Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization. Fertility And Sterility 2008, 90: 2183-2189. PMID: 18842260, DOI: 10.1016/j.fertnstert.2008.07.1739.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersCulture MediaEmbryo Culture TechniquesEmbryo ImplantationEmbryo TransferEmbryo, MammalianEmbryonic DevelopmentFemaleFertilization in VitroHumansLive BirthMagnetic Resonance SpectroscopyMetabolomicsPredictive Value of TestsPregnancyPregnancy RateProtonsRetrospective StudiesSensitivity and SpecificityTreatment OutcomeConceptsEmbryo culture mediumNoninvasive metabolomic profilingMetabolomic profilesMetabolomic profilingReproductive technology programViability indexNuclear magnetic resonance metabolomic profilingRetrospective studyART treatmentMAIN OUTCOMEReproductive outcomesCulture mediumDay 3NMR metabolomic profilesGlutamate concentrationMultivariate analysisPregnancyAssessment of embryo viability in assisted reproductive technology: shortcomings of current approaches and the emerging role of metabolomics
Bromer JG, Seli E. Assessment of embryo viability in assisted reproductive technology: shortcomings of current approaches and the emerging role of metabolomics. Current Opinion In Obstetrics & Gynecology 2008, 20: 234-241. PMID: 18460937, DOI: 10.1097/gco.0b013e3282fe723d.Peer-Reviewed Original ResearchConceptsHigher multiple pregnancy rateEmbryo assessmentMultiple pregnancy rateReproductive technology cyclesLower implantation rateReproductive technologiesAssessment of glucoseCleavage rateImplantation rateUse of metabolomicsPregnancy rateRole of metabolomicsEmbryo culture mediumEmbryo viabilityUseful adjunctGood embryosAmino acid metabolismReproductive medicineMetabolomic profilingTechnology cyclesAcid metabolismOxygen consumptionOverview of studiesEmbryo metabolismProteomic profilingNoninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study
Scott R, Seli E, Miller K, Sakkas D, Scott K, Burns DH. Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study. Fertility And Sterility 2008, 90: 77-83. PMID: 18281045, DOI: 10.1016/j.fertnstert.2007.11.058.Peer-Reviewed Original ResearchConceptsMetabolomic profilingProspective blinded pilot studyNoninvasive metabolomic profilingEmbryonic reproductive potentialBlinded pilot studyReproductive technology programCharacteristic curve analysisHuman embryo culture mediaOverall diagnostic accuracyViability indexImplantation rateEmbryo culture mediumMAIN OUTCOMEDay 3Noninvasive assessmentDay 5Culture mediumDiagnostic accuracyPilot studyCurve analysisSimilar findingsReproductive potentialRapid noninvasive assessmentDelivery rateHuman embryos