Mitochondrial function in women with polycystic ovary syndrome.
Cozzolino M, Seli E. Mitochondrial function in women with polycystic ovary syndrome. Current Opinion In Obstetrics & Gynecology 2020, 32: 205-212. PMID: 32068544, DOI: 10.1097/gco.0000000000000619.Peer-Reviewed Original ResearchConceptsPolycystic ovary syndromeLower mtDNA copy numberOvary syndromePathogenesis of PCOSMitochondrial dysfunctionMtDNA copy numberPotential protective rolePCOS phenotypeInsulin resistancePathophysiological mechanismsCommon findingDiagnostic modalitiesMetabolic milieuFunctional alterationsMitochondrial functional alterationsProtective roleCommon diseaseOxidative stressWomenMitochondrial functionDysfunctionSyndromeCopy numberProtein responseReactive oxygenMitochondrial DNA content decreases during in vitro human embryo development: insights into mitochondrial DNA variation in preimplantation embryos donated for research
Pérez-Sánchez M, Díez-Juan A, Beltrán D, Mifsud A, Mercader A, Vidal C, Labarta E, Pellicer A, Seli E, De Los Santos MJ. Mitochondrial DNA content decreases during in vitro human embryo development: insights into mitochondrial DNA variation in preimplantation embryos donated for research. F&S Science 2020, 1: 36-45. PMID: 35559739, DOI: 10.1016/j.xfss.2020.05.001.Peer-Reviewed Original ResearchDay 3 embryosMetaphase II oocytesMtDNA contentII oocytesFailed-fertilized oocytesMtDNA copy numberProspective cohort studySignificant decreaseHuman metaphase II oocytesQuantitative polymerase chain reactionCohort studyFertilization patientsDNA loadPolymerase chain reactionPreimplantation embryo developmentAneuploid blastocystsOocyte donorsTotal mtDNA copy numberHuman oocytesEmbryo culture conditionsHuman embryo developmentCategorical variablesEmbryo developmentChain reactionMitochondrial DNA content is not predictive of reproductive competence in euploid blastocysts
Scott RT, Sun L, Zhan Y, Marin D, Tao X, Seli E. Mitochondrial DNA content is not predictive of reproductive competence in euploid blastocysts. Reproductive BioMedicine Online 2020, 41: 183-190. PMID: 32600944, DOI: 10.1016/j.rbmo.2020.04.011.Peer-Reviewed Original ResearchConceptsRelative mtDNA copy numberSingle embryo transferMitochondrial DNA copy numberMtDNA copy numberEmbryo transferSuccessful single embryo transferDNA copy numberRelative mtDNA levelsHuman blastocystsReal-time polymerase chain reactionQuantitative real-time polymerase chain reactionReproductive competenceIVF outcomesMaternal ageOngoing implantationPolymerase chain reactionEuploid blastocystsMitochondrial DNA contentReproductive outcomesSame patientClinical decisionTrophectoderm biopsySame cohortAmount of mtDNACopy number