2024
Addition of rapamycin or co-culture with cumulus cells from younger reproductive age women does not improve rescue in vitro oocyte maturation or euploidy rates in older reproductive age women
Esbert M, Tao X, Ballesteros A, Yildirim R, Scott R, Seli E. Addition of rapamycin or co-culture with cumulus cells from younger reproductive age women does not improve rescue in vitro oocyte maturation or euploidy rates in older reproductive age women. Molecular Human Reproduction 2024, 30: gaad048. PMID: 38180884, DOI: 10.1093/molehr/gaad048.Peer-Reviewed Original ResearchReproductive-age womenEuploidy rateCumulus cellsAutologous cumulus cellsAge womenReproductive ageRescue IVMOlder womenYoung donorsYoung reproductive age womenYoung womenOlder reproductive age womenOocyte maturationMetaphase II oocytesGerminal vesicle stage oocytesPolar bodyMaternal ageEuploid oocytesFirst polar bodyAddition of rapamycinHours of cultureII oocytesWomenCytogenetic constitutionRapamycin
2022
Targeted Deletion of Mitofusin 1 and Mitofusin 2 Causes Female Infertility and Loss of Follicular Reserve
Cozzolino M, Ergun Y, Seli E. Targeted Deletion of Mitofusin 1 and Mitofusin 2 Causes Female Infertility and Loss of Follicular Reserve. Reproductive Sciences 2022, 30: 560-568. PMID: 35739352, DOI: 10.1007/s43032-022-01014-w.Peer-Reviewed Original ResearchConceptsMitofusin 1Mitofusin 2Double deletionFemale reproductive competencePotential functional redundancyDynamic organellesCellular homeostasisFunctional redundancyMitochondrial dynamicsEnvironmental stressMitochondrial functionMitochondrial dysfunctionMfn1Reproductive competenceTargeted deletionMfn2Oocyte maturationDeletionCritical roleReproductive agingFemale infertilityOocytesOocyte qualityFusion mechanismMitofusinsMitochondrial dysfunction caused by targeted deletion of Mfn1 does not result in telomere shortening in oocytes.
Cozzolino M, Seli E. Mitochondrial dysfunction caused by targeted deletion of Mfn1 does not result in telomere shortening in oocytes. Zygote 2022, 30: 735-737. PMID: 35730364, DOI: 10.1017/s0967199422000089.Peer-Reviewed Original ResearchConceptsMitochondrial dysfunctionMaintenance of telomeresTargeted deletionEnd-protection functionTTAGGG repeatsMitochondrial fusionTelomeric repeatsSomatic cellsMitofusin 1Reactive oxygen speciesEnzyme complexWild-type miceOocyte growthDNA damageMouse oocytesTelomerase activityOocyte maturationDeletionFollicular depletionOxygen speciesTelomere lengthTelomeresFollicular developmentOocytesRepeats
2019
Mitofusin 1 is required for female fertility and to maintain ovarian follicular reserve
Zhang M, Bener MB, Jiang Z, Wang T, Esencan E, Scott III R, Horvath T, Seli E. Mitofusin 1 is required for female fertility and to maintain ovarian follicular reserve. Cell Death & Disease 2019, 10: 560. PMID: 31332167, PMCID: PMC6646343, DOI: 10.1038/s41419-019-1799-3.Peer-Reviewed Original ResearchConceptsOocyte-granulosa cell communicationDynamic organellesAccumulation of ceramideFemale reproductive agingMitofusin 1Secondary follicle stageMitochondrial dynamicsCell communicationReproductive phenotypesCeramide synthesis inhibitor myriocinDevelopmental arrestApoptotic cell lossMitochondrial dysfunctionTargeted deletionOvarian follicular reserveOocyte maturationFemale fertilityFollicle stageDeletionPhenotypeReproductive agingOocytesCadherinFollicular reserveOrganellesMitofusin 2 plays a role in oocyte and follicle development, and is required to maintain ovarian follicular reserve during reproductive aging
Zhang M, Bener MB, Jiang Z, Wang T, Esencan E, Scott R, Horvath T, Seli E. Mitofusin 2 plays a role in oocyte and follicle development, and is required to maintain ovarian follicular reserve during reproductive aging. Aging 2019, 11: 3919-3938. PMID: 31204316, PMCID: PMC6628992, DOI: 10.18632/aging.102024.Peer-Reviewed Original ResearchConceptsMitofusin 2Key regulatory proteinsImpaired oocyte maturationFollicle developmentMitochondrial fusionRegulatory proteinsEndoplasmic reticulumMitochondrial dysfunctionTargeted deletionOocyte maturationOocytesReproductive agingFemale subfertilityOocyte qualityOvarian follicular reserveTelomeresMitochondriaMetabolic milieuProteinReticulumDeletionFusionPhenotypeApoptosisMaturationTranslational activation of maternally derived mRNAs in oocytes and early embryos and the role of embryonic poly(A) binding protein (EPAB)
Esencan E, Kallen A, Zhang M, Seli E. Translational activation of maternally derived mRNAs in oocytes and early embryos and the role of embryonic poly(A) binding protein (EPAB). Biology Of Reproduction 2019, 100: 1147-1157. PMID: 30806655, PMCID: PMC8127035, DOI: 10.1093/biolre/ioz034.Peer-Reviewed Original ResearchConceptsTranslational activationBinding proteinSpecific protein complexesTranslation of mRNAsOocyte maturationCis-acting sequencesEarly embryo developmentProtein complexesXenopus modelEarly embryosKey regulatorGene expressionMolecular mechanismsEmbryo developmentTargeted disruptionMechanistic detailsProteinEarly developmentMRNAMice resultsKey mechanismOocytesActivationMaturationTranscription
2018
The role of mitochondrial activity in female fertility and assisted reproductive technologies: overview and current insights
Cecchino GN, Seli E, Alves da Motta E, García-Velasco J. The role of mitochondrial activity in female fertility and assisted reproductive technologies: overview and current insights. Reproductive BioMedicine Online 2018, 36: 686-697. PMID: 29598846, DOI: 10.1016/j.rbmo.2018.02.007.Peer-Reviewed Original ResearchConceptsMetabolic stress modelsMitochondrial functionFemale fertilityFemale reproductive processesPoor outcomeReplacement therapyOvarian agingMitochondrial DNA contentInfertility treatmentTherapeutic attemptsOocyte qualityClinical implicationsEmbryo potentialOocyte maturationReproductive processesPrecursor cellsEarly embryo developmentReproductive technologiesDisease-causing mutationsMitochondrial capacityRole of mitochondriaMitochondrial impactCurrent insightsTrophectoderm cellsWomen
2015
Embryonic Poly(A)-Binding Protein (EPAB) Is Required for Granulosa Cell EGF Signaling and Cumulus Expansion in Female Mice
Yang CR, Lowther KM, Lalioti MD, Seli E. Embryonic Poly(A)-Binding Protein (EPAB) Is Required for Granulosa Cell EGF Signaling and Cumulus Expansion in Female Mice. Endocrinology 2015, 157: 405-416. PMID: 26492470, PMCID: PMC4701890, DOI: 10.1210/en.2015-1135.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Morphogenetic Protein 15Cell ProliferationCells, CulturedCoculture TechniquesCumulus CellsEpidermal Growth FactorErbB ReceptorsFemaleGranulosa CellsGrowth Differentiation Factor 9Luteinizing HormoneMAP Kinase Signaling SystemMice, KnockoutOocytesPhosphorylationPoly(A)-Binding ProteinsProtein Processing, Post-TranslationalReceptors, LHSignal TransductionConceptsEpidermal growth factorZygotic genome activationP90 ribosomal S6 kinaseBone morphogenetic protein 15Cumulus cellsRibosomal S6 kinaseImpaired oocyte maturationCumulus expansionGrowth differentiation factor 9Genome activationDifferentiation factor 9S6 kinaseEarly embryosTranslational activationEGF signalingEGF receptorFemale micePhosphorylated MEK1/2EGF treatmentBinding proteinFactor 9Cells exhibitOocyte maturationProteinProtein 15Oocyte mitochondrial function and reproduction
Babayev E, Seli E. Oocyte mitochondrial function and reproduction. Current Opinion In Obstetrics & Gynecology 2015, 27: 175-181. PMID: 25719756, PMCID: PMC4590773, DOI: 10.1097/gco.0000000000000164.Peer-Reviewed Original ResearchConceptsOocyte mitochondrial functionMitochondrial functionEmbryonic developmentMitochondrial nutrientsMitochondrial replacementRole of mitochondriaOogonial stem cellsCellular organellesMammalian reproductionMitochondrial performanceMitochondrial diseaseEmbryo developmentMitochondrial activityMitochondrial dysfunctionOocyte developmentMitochondriaReproductive consequencesStem cellsOocyte maturationMitochondrial abnormalitiesPolar bodyReproductionEnergy productionIntake of compoundsNegative long-term health effects
2013
Human embryonic poly(A)-binding protein (EPAB) alternative splicing is differentially regulated in human oocytes and embryos
Guzeloglu-Kayisli O, Lalioti MD, Babayev E, Torrealday S, Karakaya C, Seli E. Human embryonic poly(A)-binding protein (EPAB) alternative splicing is differentially regulated in human oocytes and embryos. Molecular Human Reproduction 2013, 20: 59-65. PMID: 24002949, DOI: 10.1093/molehr/gat061.Peer-Reviewed Original ResearchConceptsZygotic genome activationEarly embryo developmentEarly embryosTranslational activationSomatic tissuesSpliced formsEmbryo developmentEmbryo-specific expressionPost-transcriptional mechanismsOocyte maturationHuman somatic tissuesFull-length formGenome activationExon 8Transcriptional regulationAlternative splicingTranscriptional activityGene expressionHuman oocytesEPABAmino acidsXenopusEmbryosMRNAOocytes
2012
Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice
Guzeloglu-Kayisli O, Lalioti MD, Aydiner F, Sasson I, Ilbay O, Sakkas D, Lowther KM, Mehlmann LM, Seli E. Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice. Biochemical Journal 2012, 446: 47-58. PMID: 22621333, PMCID: PMC3955213, DOI: 10.1042/bj20120467.Peer-Reviewed Original ResearchConceptsTranslational activationOocyte maturationZygotic genome activationFemale fertilityGenome activationGerminal vesicle stage oocytesDazl mRNAEarly embryogenesisMaternal mRNAsDownstream regulatorsMammalian reproductionGene expressionEPABStage oocytesXenopus oocytesEpidermal growthLate antral folliclesOogenesisMRNAOocytesMaturationProteinFemale miceCumulus expansionAntral folliclesEmbryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation
Friend K, Brook M, Bezirci FB, Sheets MD, Gray NK, Seli E. Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation. Biochemical Journal 2012, 445: 93-100. PMID: 22497250, PMCID: PMC3955212, DOI: 10.1042/bj20120304.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCytoplasmFemaleFluorescent Antibody TechniqueGene Expression Regulation, DevelopmentalImmunoprecipitationMutationOocytesOogenesisPhosphorylationPoly APoly(A)-Binding ProteinsPolyadenylationProtein BiosynthesisProtein Processing, Post-TranslationalRNA, MessengerXenopus laevisXenopus ProteinsConceptsCytoplasmic polyadenylationOocyte maturationPost-transcriptional regulatorsEarly embryonic developmentPost-translational modificationsXenopus oocyte maturationMaternal mRNAsProtein phosphorylationResidue clustersTranslational activationEmbryonic developmentXenopus laevis oocytesProtein activityPhosphorylationFirst insightLaevis oocytesPolyadenylationInherent abilityEPABMaturationPhosphoproteinRegulatorMutationsMRNAOocytes