2024
Mitochondria as determinants of reproductive senescence and competence: implications for diagnosis of embryo competence in assisted reproduction
Yildirim R, Seli E. Mitochondria as determinants of reproductive senescence and competence: implications for diagnosis of embryo competence in assisted reproduction. Human Reproduction 2024, 39: 2160-2170. PMID: 39066612, DOI: 10.1093/humrep/deae171.Peer-Reviewed Original ResearchMultiple quality control mechanismsMitochondrial unfolded protein responseMitochondrial DNA copy numberUnfolded protein responseQuality control mechanismsDNA copy numberMitochondrial networkRegulate calcium signalingMitochondrial dynamicsReproductive successMitochondrial quantityProtein responseDinucleotide contentModulate apoptosisOxidative phosphorylationCellular pathwaysCopy numberMitochondrial dysfunctionMitochondriaCalcium signalingEmbryo competenceMembrane potentialAssisted reproductionDiagnostic targetsReproductive senescence
2019
Mitochondrial unfolded protein response: a stress response with implications for fertility and reproductive aging
Seli E, Wang T, Horvath TL. Mitochondrial unfolded protein response: a stress response with implications for fertility and reproductive aging. Fertility And Sterility 2019, 111: 197-204. PMID: 30691623, DOI: 10.1016/j.fertnstert.2018.11.048.Peer-Reviewed Original ResearchConceptsMitochondrial unfolded protein responseTwo-cell embryo developmentUnfolded protein responseImpaired oocyte maturationMorphology of mitochondriaMitochondrial dysfunction resultsPremature reproductive agingNovel mechanistic insightsMitochondrial DNA contentReactive oxygen species productionPrevention of agingCLPP resultsProtein responseOxygen species productionReproductive agingPreimplantation embryosAge-related accumulationOxidative phosphorylationStress responseEmbryo developmentForm blastocystsMitochondrial functionMitochondriaMitochondrial dysfunctionEnergy metabolismMetabolic imaging via fluorescence lifetime imaging microscopy for egg and embryo assessment
Sanchez T, Zhang M, Needleman D, Seli E. Metabolic imaging via fluorescence lifetime imaging microscopy for egg and embryo assessment. Fertility And Sterility 2019, 111: 212-218. PMID: 30691624, DOI: 10.1016/j.fertnstert.2018.12.014.Peer-Reviewed Original ResearchConceptsMetabolic imagingInvasive diagnostic interventionsEmbryo assessmentPreimplantation genetic testingUseful diagnostic methodAssisted reproductive technology (ART) laboratoryClinical benefitSingle ETDiagnostic interventionsOxidative phosphorylationGenetic testingReproductive technology laboratoriesExperimental modelCopy number assessmentMetabolic functionsMorphologic parametersFluorescence lifetimeEmbryosClinical applicationMetabolic stateDiagnostic methodsEmbryo viabilityElectron transporterCentral roleCurrent strategies