Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization
Seli E, Gardner DK, Schoolcraft WB, Moffatt O, Sakkas D. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertility And Sterility 2004, 82: 378-383. PMID: 15302287, DOI: 10.1016/j.fertnstert.2003.12.039.Peer-Reviewed Original ResearchConceptsBlastocyst developmentInfertility treatmentPregnancy rateTUNEL positivityIVF clinicsDNA strand breaksPrivate IVF clinicClinical pregnancy rateProspective comparative studyNick end labeling techniqueBlastocyst development ratePregnancy outcomesVs. 44MAIN OUTCOMESperm samplesLarge seriesSignificant negative correlationStrand breaksFlow cytometryTUNEL reactivityEnd-labeling techniqueIVFApoptotic cell deathPositivityNuclear DNA fragmentation